Dock protein family in brain development and neurological disease.

The family of dedicator of cytokinesis (Dock), a protein family that belongs to the atypical Rho guanine nucleotide exchange factors (GEFs) for Rac and/or Cdc42 GTPases, plays pivotal roles in various processes of brain development. To date, 11 members of Docks have been identified in the mammalian system. Emerging evidence has suggested that members of the Dock family are associated with several neurodegenerative and neuropsychiatric diseases, including Alzheimer disease and autism spectrum disorders. This review summarizes recent advances on the understanding of the roles of the Dock protein family in normal and diseased processes in the nervous system. Furthermore, interacting proteins and the molecular regulation of Docks are discussed.

Introduction of Dock protein family

The evolutionarily conserved Dock protein family is a newly characterized family of atypical Rho guanine nucleotide exchange factor (GEF) for Rac and/or Cdc42 GTPases., The typical Dbl family of Rho GEFs possesses a pleckstrin homology (PH)-Dbl homology (DH) module, of which PH domain is important for the phospholipid-binding and membrane targeting of Dbl GEFs, and DH domain is responsible for its GEF activity. By contrast, Dock family lacks the PH-DH module, but instead contains a Dock homology region (DHR) 1-DHR2 module. DHR1-DHR2 module plays similar roles as PH-DH module, of which DHR1 is important for the phospholipid-binding and membrane targeting of Docks, and DHR2 is responsible for their GEF activity.

To date, 11 members of Docks, namely Dock1 (Dock180) to 11, have been identified in mammalian system. Based on sequence homology, these Docks are divided into 4 subfamilies: Dock-A, which includes Dock180, Dock2, and Dock5; Dock-B, which includes Dock3 and Dock4; Dock-C (also called zizimin-related, or zir family), which includes Dock6, Dock7, and Dock8; and Dock-D (also called zizimin family), which includes Dock9, Dock10, and Dock11 (Fig. 1)., In addition to the DHR1-DHR2 module, Dock-A and -B members contain an N-terminal Src homology 3 (SH3) domain and a proline-rich C-terminus, thus are more phylogenetic related to each other. On the other hand, Dock-C members lack recognizable domains outside of DHR1-DHR2 module, whereas Dock-D members contain an N-terminal PH domain. Both Dock-A and -B members preferentially activate Rac, whereas Dock-D members preferentially activate Cdc42. Dock-C members do not show unified GEF activity, i.e., Dock6 and Dock7 are capable of activating both Rac or Cdc42, whereas Dock8 preferentially activates Cdc42.

Figure 1. Schematic structure of different members of Dock protein family. Dock family proteins are divided into 4 subfamilies, Dock-A–D. Members of each subfamily and their alternate names are listed (MOCA, modifier of cell adhesion; PBP, presenilin-binding protein; Zir, zizimin-related). Structure of different domains, including SH3 (Src Homology 3), PH (pleckstrin homology), DHR (Dock homology region) 1, DHR2, and the proline-rich region (PxxP) are indicated.

Members of Dock protein family have been found to play important roles in multiple processes of brain development, including the development and functioning of neurons, microglia, and Schwann cells. Notably, emerging evidence has linked Docks with neuropsychiatric and neurodegenerative disorders, including autism spectrum disorders, schizophrenia, and Alzheimer and Parkinson diseases (AD and PD, respectively; Table 1). This review summarizes the current understanding of the roles of Dock protein family in nervous system during physiological and pathological conditions.

Table 1. Function of Dock proteins in nervous system and their related neurological diseases

	Function	Related neurological diseases	Ref.
Dock180	Axon pathfinding, dendritic spine morphogenesis		10–12,14
Dock2	Neuroinflammation, microglial function	Alzheimer disease	19, 20
Dock3	Axonal growth and regeneration, neurite outgrowth, neuroprotection	Alzheimer disease, attention deficit hyperactivity disorder	21–32
Dock4	Neurite differentiation, dendritic spine morphogenesis	Autism, dyslexia, schizophrenia	34–40
Dock5		Parkinson disease	6
Dock6	Neurite outgrowth, axon growth and regeneration		43, 44
Dock7	Neuronal polarization, cortical neurogenesis, Schwann cell development		45, 46, 48, 49
Dock8		Mental retardation, autism	55, 56
Dock9	Dendrite development	Bipolar disorder	58, 59
Dock10	Neurite dynamics	Autism	60, 61

Function of Dock protein family in nervous system and its related neurological diseases

Dock-A and Dock-B

Dock-A and -B members are the most studied Dock proteins in nervous and other systems. The neural functions of Dock1–4 have been revealed by multiple in vitro and in vivo studies. The neural function of Dock5, however, has remained to be explored, although evidence from genetic studies has implicated that Dock5 may be associated with PD. It was found that members of these 2 subfamilies form evolutionarily conserved associations with 2 groups of adaptor proteins, engulfment and cell motility (ELMO) and CT10 regulator of kinase (Crk) adaptor proteins. Formation of the bipartite complex of Docks and ELMO is one of the most important regulatory ways to activate the GEF activity of Dock-A and -B members toward Rac.,

Dock180

As the first identified member of Dock protein family, Dock180 has been found to play diverse roles in phagocytosis and cell migration. In nervous system, Dock180 is essentially involved in the regulation of axon guidance and dendritic spine morphogenesis. Dock180 binds to the netrin receptor DCC (deleted in colorectal cancer) and mediates netrin-induced Rac1 activation and axon growth. Such regulation is important for the commissural axon projection. Interestingly, Dock180 is also important for the axon pruning induced by ephrin-B3 reverse signaling and RhoG., Dock180 couples to ephrin-B3 through interacting with the adaptor protein Grb4/Nck2, and hence mediating ephrin-B3 signaling toward Rac1 activation and pruning of hippocampal mossy fiber axons., Moreover, the Dock180-ELMO complex participates in RhoG-mediated reduction of axonal complexity. The bi-faced roles of Dock180 in axon attraction or repulsion suggests that precise regulation of Dock180 at the axonal growth cones is an essential molecular control of the axon tip motility. Dock180 is also found to be involved in synapse development. The RhoG-ELMO1-Dock180 complex promotes Rac1 activation and leads to dendritic spine morphogenesis in hippocampal neurons.

Although Dock180 plays critical roles in these neural developmental processes, it has not been reported that Dock180 itself is linked to neurological diseases. However, brain-specific angiogenesis inhibitor-1 (BAI-1), an interacting protein of Dock180-ELMO module, has been found to be linked to schizophrenia, bipolar disorder, and addiction., As BAI-1 regulates dendrite morphogenesis and synaptogenesis,, it is of interest to explore whether Dock180 modulates the neural function of BAI-1 and whether such regulation is implicated in the pathogenesis of neuropsychiatric disorders.

Dock2

Dock2 is highly expressed in the immune system and regulates immune-cell functions. In brain, Dock2 is expressed exclusively in microglia and is implicated in neuroinflammation of AD pathology., It has been shown that the number of Dock2-expressing microglia is abnormally increased in brains of AD patients. The expression of Dock2 is positively regulated by prostaglandin E2 receptors, which mediate inflammatory, neurotoxic, and amyloidogenic effects induced by the increased secretion of microglial prostaglandins during AD pathogenesis. Importantly, Dock2 deficiency significantly reduces the area and size of β-amyloid (Aβ) plaque in cerebral cortex and hippocampus of a mouse model of AD. Thus, Dock2 may be a key molecule that contributes to the innate immune activation and Aβ plaque burden in AD.

Dock3

Dock3 was first identified as a presenilin-binding protein (PBP), and is found to be localized to the particulate fraction of sporadic AD brains. Multiple lines of evidence have suggested a complex mechanism involved in Dock3 signaling that contributes to AD pathogenesis. First, Dock3 is associated with neurofibrillary tangles and promotes the phosphorylation of tau protein. Second, Dock3 is shown to integrate the neuronal death signals transduced from familial AD-linked amyloid β precursor protein (APP) and presenilin (PS) mutants. Both of these findings point to a role of Dock3 in the neurodegenerative process in AD. On the other hand, studies from different groups have demonstrated neuroprotective roles of Dock3. First, Dock3 decreases the secretion of APP and Aβ peptide by accelerating the proteasome-dependent degradation of APP. Second, Dock3 ameliorates the neurotoxicity induced by N-methyl-D-aspartate receptors (NMDARs) via interacting with the C-terminus of NMDAR subunits., Third, Dock3 is important for maintaining the functional integrity of axons, as loss of Dock3 leads to axon degeneration. Given that Dock3 is appeared to play dual roles in neural degeneration and protection, further analysis on its precise temporal and spatial regulation is required for the understanding of Dock3′s role in AD pathology. In addition to AD, Dock3 has also been implicated to link to psychiatric disorders such as attention deficit hyperactivity disorder.

Studies from in vivo and in vitro experiments have demonstrated that a major neural function of Dock3 is to promote neurite and axonal growth. Several molecular mechanisms have been revealed underlying Dock3-mediated neurite and axon growth. First, Dock3 associates with ELMO and RhoG to form the conventional ternary Dock-ELMO-RhoG complex, which is important for Rac1 activation during brain-derived neurotrophic factor (BDNF)-TrkB mediated neurite outgrowth. Moreover, Dock3 regulates actin cytoskeleton, microtubule assembly, and cell–cell adhesion by interacting with or regulating WAVE (Wiskott-Aldrich syndrome protein family verprolin-homologous), GSK-3β (glycogen synthase kinase 3β), and N-cadherin, respectively.- Importantly, these Dock3-regulated molecular events all participate in BDNF-induced neurite outgrowth. Dock3 is also found as a negative regulator of Wnt/β-catenin signaling, as Dock3 inhibits the nuclear expression of β-catenin.

Dock4

The gene encoding Dock4 has been found to be associated with several neuropsychiatric diseases, including autism, dyslexia, and schizophrenia.- Of note, a rare heterozygous microdeletion found to be associated with autism and dyslexia leads to a fusion transcript that generates a shorter Dock4 protein product lacking the complete DHR2 domain and the C-terminus., Indeed, the DHR2-dependent Rac1 activation and actin organization is important for Dock4 in regulating the neurite differentiation of neuroblastoma cells. The SH3-dependent interaction of Dock4 with ELMO2 is important for this regulation, whereas the C-terminus of Dock4 is dispensable. In hippocampal neurons, Dock4 regulates the establishment of axon-dendrite polarity and dendrite arborization, which is also dependent on the SH3 domain and GEF activity of Dock4. Interestingly, the C-terminus of Dock4 is not important for polarity establishment, but may play regulatory roles in dendrite development., Furthermore, Dock4 is expressed in dendritic spines and participates in spine morphogenesis that dependent on its GEF activity and C-terminus. Importantly, the C-terminus of Dock4 was shown to both regulate the synaptic localization of Dock4 and mediate the interaction with the actin-binding protein cortactin. It is thus of interest to further explore the detailed role of the C-terminus of Dock4 during spine morphogenesis. Among all Docks, Dock4 is the only member that is also capable of activating Rap1, a Ras-related small GTPase involved in neuronal migration and spine dynamics., Whether the regulation of Rap1 is important for Dock4-dependent neural functions awaits further study.

Dock-C

Dock-C members, also called zizimin-related proteins, play regulatory roles in both central and peripheral nervous systems. In comparison with Dock-A and -B, Dock-C members do not interact with ELMO and Crk adaptors.

Dock6

Dock6 was found to promote neurite outgrowth and regulate axonal growth and regeneration of sensory neurons., Although Dock6 is capable of activating both Rac1 and Cdc42 in vitro, it preferentially activates Rac1 in dorsal root ganglion (DRG) neurons. Importantly, the GEF activity of Dock6 toward Rac1 is negatively regulated by Akt-dependent phosphorylation at Ser1194. During the initiation of axon growth at embryonic stages or after injury, Dock6 interacts with the protein phosphatase PP2A, which dephosphorylates Dock6 at Ser1194 and activates its GEF activity and axon growth. In later developmental stages, Dock6 switches to bind to Akt, which phosphorylates Dock6 and inhibits its GEF activity. This Akt-dependent phosphorylation of Dock6 is regulated by nerve-derived factor (NGF)-TrkA signaling and phosphoinositide 3-kinase. Re-introducing a nonphosphorylatable mutant (Ser1194A) and a phosphomimetic mutant (S1194E) in mice lacking Dock6 provides in vivo evidence that the phosphorylation status of Dock6 is a molecular determinant for axon growth.

Dock7

Dock7 is highly expressed in the developing brain and has been found to play important roles in several neuronal developmental processes., First, Dock7 regulates the neurogenesis in the neocortex by promoting the differentiation of radial glial progenitor cells (RGCs) to basal progenitors and neurons. Interestingly, such regulation is not dependent on the GEF activity of Dock7, but is through interaction with the microtubule- and centrosome-associating protein TACC3 (transforming acidic coiled-coil-containing protein 3). Binding to Dock7 inhibits the function of TACC3 on microtubule growth, hence promoting the interkinetic nuclear migration of RGCs and cortical neurogenesis. A second neural function of Dock7 in the central nervous system is the regulation of neuronal polarity and axon formation. Dock7 is preferentially expressed in the axons of developing hippocampal neurons, where it activates Rac and promotes axon formation. A microtubule destabilizing protein stathmin/Op18 is inactivated by Dock7 and Rac, which is a critical molecular event toward modulating the microtubule network during Dock7-regulated axon formation. Furthermore, Dock7 is found to form a complex with myosin VI in neuronal cells, and may thus be implicated in the regulation of myosin VI-dependent motor transport or actin cytoskeletal organization in neurons.

In the peripheral nervous system, Dock7 is important for the development of Schwann cells, the glial cells that ensheath the axons of motor and sensory neurons., Dock7 negatively regulates the differentiation of Schwann cells and the onset of myelination in both primary Schwann cells in vitro and sciatic nerves in vivo. Knockdown of Dock7 leads to a downregulation of Rac1 and Cdc42, concomitant with an activation of RhoA. Although Dock7 is a negative regulator for Schwann cell differentiation, it promotes Schwann cell migration mediated by neuregulin. Dock7 directly binds to the neuregulin receptor ErbB2, which activates the GEF activity of Dock7 by phosphorylating it at Tyr1118.

Dock7 has been found as an interacting partner of tuberous sclerosis complex 1 (TSC1) and TSC2., Mutations of the genes encoding TSC1 and TSC2 are the main causes of multi-system benign tumors in tuberous sclerosis, and are also associated with neural developmental diseases, including mental retardation and epilepsy. It is of importance to investigate whether Dock7 regulates the function of TSC1/2 and whether such regulation is implicated in neural developmental diseases. Interestingly, despite Dock7 has multiple roles in neuronal development and Schwann cell myelination, a study reported that 2 mice with Dock7 mutations resulted from a chemical mutagenesis screen exhibited normal activities in several neurobehavioral studies, including tests for depressive and anxiety-like behaviors, memory, and locomotion. It is possible that other Docks may play redundant roles to compensate the loss-of-function of Dock7. Nonetheless, a more detailed molecular and behavioral analysis on specific neural function, such as cognition, will be required to determine the in vivo role of Dock7.

Dock8

Dock8 is highly expressed in the immune system, and Dock8 deficiency causes immune-related disorders. Not like other Dock-C members, Dock8 exhibits specific activity toward Cdc42 but not Rac1.,, Importantly, Dock8 is expressed in multiple regions of human brain, and mutations of the gene encoding Dock8 has been found in mental retardation and autism patients., This suggests that Dock8 may play important neural functions, which need further investigation.

Dock-D

Dock-D members, also called zizimin family proteins, exhibit specific GEF activity toward Cdc42. The biological functions of Dock-D members have been relatively less studied. Although Dock9 and Dock10 expressions are detected in brain, the neural function of them is only beginning to be understood. On the other hand, Dock11 is expressed predominantly in lymphocytes, and has not been found to play roles in brain yet.

Dock9

Dock9 is expressed in brain at later developmental stages and is important for dendrite development. The GEF activity of Dock9 toward Cdc42 activation is important for dendritic outgrowth of cultured hippocampal neurons. Moreover, the PH domain and the DHR1 domain of Dock9 are important for its membrane targeting and activation of Cdc42. It is noteworthy that sequence variations have been found in the gene encoding Dock9 in bipolar disorder patients, and mutations of Dock9 gene are associated with several typical symptoms of the disease. This suggests that Dock9 may contribute to both risk and increased illness severity in bipolar disorder.

Dock10

Variations of Dock10 gene have been found in patients of autism spectrum disorders. Although the neural function of Dock10 is not clearly understood, it was found to be highly expressed in neurites of neuroblastoma cells and implicated in the extension/retraction dynamics of neurites.

Molecular function and regulation of Dock protein family

Dock proteins act in both GEF-dependent and -independent ways

The activation of Docks toward Rac1 and/or Cdc42 is a classical function of Dock protein family in modulating actin dynamics. However, Docks can also act in a GEF-independent way during several neuronal developmental processes. For example, Docks can interact with the actin-binding proteins WAVE, WASP (Wiskott-Aldrich syndrome protein), and cortactin to regulate actin organization independent of their GEF activity.,, Docks can also regulate microtubule dynamics through binding to the microtubule-regulating proteins GSK-3β and TACC3., Moreover, Docks can regulate assembly, cellular localization, and degradation of other signaling molecules, such as Wnt signaling molecules and NMDA receptors.,, Table 2 summarizes the interacting proteins of Docks found in nervous and other systems.-

Table 2. A summary of interacting proteins of Dock1–8*,#

	Interacting proteins	Domains of Docks for interaction	Regulatory roles	Function	Ref.
Dock180	ELMO1	1–161 a.a., containing SH3	Increasing GEF activity of Dock180 toward Rac1	Dendritic spine morphogenesis, reducing axonal complexity	67
	CrkII	1752–1865 a.a. of the C-terminus	Increasing GEF activity of Dock180 toward Rac1	Cell migration	9, 69
	Grb4/Nck2	1793–1952 a.a. of the C-terminus	Mediating the recruitment of Dock180 to activated ephrin-B3	Axon pruning	11, 13
	DCC		Increasing GEF activity of Dock180 toward Rac1	Axon guidance	10
	GRASP/Tamalin	SH3	Scaffolding Dock180 to ARF-Rac signaling	Epithelial cell migration	63
	ANKN28	SH3		Cell migration and focal adhesion formation	64
	SNX5	DHR1		Endosome-to-trans-Golgi-network transport	65
	WAVE1–3	DHR1			30
	Dock5				76
Dock2	ELMO1	SH3	Increasing GEF activity of Dock2 toward Rac1	Lymphocyte migration	66
	CrkL	1–515 a.a. and 939–1476 a.a.	Increasing GEF activity of Dock2 toward Rac1	Cytoskeletal regulation in leukemia cells	70
	Vav			Cytoskeletal regulation in leukemia cells	70
	WAVE1–3	DHR1			30
Dock3	ELMO	1–160 a.a., containing SH3	Increasing GEF activity of Dock3 toward Rac1	Neurite outgrowth	29
	Presenilin			AD pathogenesis	21
	β-catenin		Inhibiting nuclear β-catenin expression	Inhibiting Wnt signaling	33
	Fyn	1773–2028 a.a. of the C-terminus	Recruiting Dock3 to activated TrkB receptors	Axonal outgrowth and regeneration	30
	WAVE1–3	DHR1	Recruiting WAVE complex to activated TrkB receptors	Axonal outgrowth and regeneration	30
	GSK-3β	1628–1777 a.a. of the C-terminus	Inhibiting GSK-3β activity by increasing its phosphorylation	Axonal outgrowth and regeneration	31
	NR2B		Increases NR2B degradation	Ameliorating NMDA-mediated neurotoxicity	26
	NR2D	796–1154 a.a. (the linker between DHR1 and DHR2 and part of the DHR2)		Ameliorating NMDA-mediated neurotoxicity	25
Dock4	ELMO2	1–161 a.a., containing SH3	Increasing GEF activity of Dock4 toward Rac1	Neurite and dendrite development	38, 39
	CrkII	C-terminus	Increasing GEF activity of Dock4 toward Rac1	Dendrite development	39
	Cortactin	C-terminus	Synaptic localization of Dock4	Dendritic spine morphogenesis	40
	APC	DHR2		Stabilizing β-catenin, cell migration	62
	Axin	C-terminus	Increasing Axin degradation	Stabilizing β-catenin, cell migration	62
	GSK-3β	C-terminus	Phosphorylation of Dock4 by GSK-3β, increasing Dock4 GEF activity toward Rac	Stabilizing β-catenin, cell migration	62
	WAVE1–3	DHR1			30
Dock5	CrkII	1736–1784 a.a. of the C-terminus		Intestinal epithelial cell spreading and migration	71
	CrkL	1738–1870 a.a. of the C-terminus		Intestinal epithelial cell spreading and migration	71
	Dock180				76
Dock6	Akt	DHR1	Inhibiting Dock6 GEF activity toward Rac1 by phosphorylating Dock6 at S1194	Axon growth and regeneration	44
	PP2A	DHR2	Increasing Dock6 GEF activity toward Rac1 by dephosphorylating Dock6 at S1194	Axon growth and regeneration	44
Dock7	TACC3	933–1164 a.a. (a region following DHR1 domain)	Antagonizing TACC3 function on microtubule growth or stabilization	Interkinetic nuclear migration of radial glial cells and cortical neurogenesis.	46
	ErbB2	692–1431 a.a. (the whole sequence between DHR1 and DHR2)	Increasing Dock7 GEF activity toward Rac1 and Cdc42 by phosphorylating Dock7 at Y1118	Schwann cell migration	48
	Myosin VI				47
	TSC1/2				50, 51
Dock8	WASP	754–1452 a.a. of the linker region between DHR1 and DHR2	Mediating the localization of WASP in immune cells	Regulating F-actin organization of immune cells.	54
	Talin	1453–2099 a.a. (C-terminus including DHR2)	Mediating the localization of talin in immuno cells	Regulating integrin-mediated adhesion of immune cells.	54

* As Rac and/or Cdc42 are known to bind to the DHR2 domain of all Docks, these 2 GTPases are not summarized in this table.

# It has been unknown for the interacting proteins of Dock9–11.

Abbreviations: ANKN28, ankyrin repeat domain 28; APC, adenomatous polyposis coli; Crk, CT10 regulator of kinase; CrkL, Crk-like; DCC, deleted in colorectal cancer; ELMO, engulfment and cell motility; GRASP, Golgi reassembly and stacking protein; GSK-3β, glycogen synthase kinase-3β; NR2B or NR2D, N-methyl-d-aspartic acid (NMDA) receptor 2B or 2D subunit; PP2A, protein phosphatase 2A; SNX5, sorting nexin 5; TACC3, transforming acidic coiled-coil-containing protein 3; TSC, tuberous sclerosis complex; WASP, Wiskott-Aldrich syndrome protein; WAVE, WASP family verprolin-homologous

Regulation of Dock Activity

Protein–protein interactions

The most well known regulation of the Dock family, in particular Dock-A and Dock-B members, is the interaction with ELMO.,,,, ELMO binds to the SH3 domain of Docks, thus leading to the release of the autoinhibitory status of Docks and exposure of their DHR2 domain for Rac activation. Moreover, ELMO acts as a scaffold protein to link Docks to other signaling molecules, such as RhoG. Crk adaptor proteins, including CrkII and CrkL, are another group of adaptors that activates the GEF activity of Dock-A and -B members through binding to their C-terminus.,- As Dock-C and Dock-D members lack the N-terminal SH3 domain and the proline-rich C-terminal region, they do not bind to ELMO and Crk adaptors. Whether common adaptor proteins bind to these 2 subfamilies of Docks to function similarly as ELMO and Crk remains to be investigated.

GEFs for Rho GTPases normally couple to membrane receptors or signaling molecules to transduce extracellular stimuli toward activation of Rho GTPases. Dock proteins participate in a number of signaling pathways, among which Trk receptors, Neuregulin-ErB, Eph-Ephrin, and Wnt mediated signaling pathways are important in nervous system.,,,,, Docks can be directly or indirectly recruited to these signaling receptor complexes to be activated (Table 2).

Homodimerization and heterodimerization

Dock1, Dock2, and Dock9 can self-dimerize to form homodimers., It has been revealed that dimerization of Dock2 does not alter its GEF activity in vitro, but is important for its function under physiological conditions. This suggests that dimerization probably increases the signaling capacity of Docks. Given that the conserved DHR2 domain mediates the self-binding, dimerization through this domain may be a general mechanism for all Docks., In addition to homodimerization, heterodimerization formed between different Dock proteins (e.g., Dock1 and Dock5) is also evident. It is thus likely that clustering of one or more Dock proteins is one mechanism to regulate the local activity of Docks.

Phospholipid-binding and membrane targeting

Structural analysis of Dock180 has identified a common C2 domain scaffold and surface loops in the DHR1 domain of all Docks, which mediates the direct binding to phosphatidylinositol (3,4,5)-trisphosphate (PtdIns(3,4,5)P3). Studies have confirmed that the DHR1-phospholipid binding is a common characteristic of Docks and such binding is important for the cellular function of Docks.,- In addition to DHR1 domain, members of Dock-D subfamily may also use the N-terminal PH domain to interact with phospholipids. The phospholipid binding regulates the function of Docks in 2 ways. First, this interaction releases the autoinhibitory structure of Docks and frees the DHR2 domain for GTPase activation. Second, this interaction translocates Dock proteins to the plasma membrane, where they locally activate Rho GTPases. Consistent with this notion, the phospholipid-binding regulated activation of GEF activity and membrane targeting is one of the important regulatory ways of Dock proteins in various biological processes, including cell polarization and migration.

Phosphorylation

Emerging evidence has identified that phosphorylation is important for the GEF activities of Dock proteins. For instance, tyrosine phosphorylation of Dock180 at multiple sites by Src kinase activates its GEF activity during tumorigenesis., Moreover, Dock4 is phosphorylated at the C-terminus by GSK-3β, which is important for Wnt-induced Rac activation. In the nervous system, 2 phosphorylation events, Ser1194 phosphorylation of Dock6 by Akt and Tyr1118 of Dock7 by ErbB2 receptors, have been found to control Dock activity during axon growth and Swchann cell migration, respectively., Interestingly, Ser1194 phosphorylation of Dock6 inhibits its activity, while Tyr1118 of Dock7 activates its activity. This suggests that phosphorylation can regulate Dock activity in both ways.

Concluding remarks

Members of Dock family play roles in diverse processes of nervous system, including the development and functioning of neurons, microglia, and Schwann cells. More importantly, many Dock proteins have been implicated in neurological diseases or associated with disease-related molecules. Nonetheless, there is still limited evidence that how deregulation of individual Dock proteins links to the system disorders in the brain. The neural function of several Docks, such as Dock5 and Dock8, has not been explored, although the genes encoding these 2 Docks have been shown to link with neurological diseases.,, Therefore, investigations on the synaptic network connectivity and neural behaviors in animals with manipulations of individual Dock genes are important to reveal the physiological roles of Docks in brain. Furthermore, this review summarizes the interacting proteins and molecular regulation of Docks. However, the detailed roles of Docks in transducing extracellular signals into actin reorganization or other cellular changes in the nervous system still remain to be fully understood.