BACKGROUND: Epigenetic alterations, including histone modifications, play an important role during carcinogenesis. This study was designed to systematically investigate histone H3K9 and H3K27 methylation levels in bladder cancer (BCa) tissue. METHODS: A tissue microarray with urothelial BCa (150 non-muscle-invasive BCa, NMIBC; 121 muscle-invasive BCa, MIBC; 31 metastatic BCa, MET) and normal urothelium (29, CTRL) specimen was used to determine the global levels of H3K9 and H3K27 mono-, di- and tri-methylation. RESULTS: Global levels of H3K9 and H3K27 methylation were significantly higher in CTRL than in BCa, and levels in NMIBC were higher compared to MIBC. Histone methylation levels of MET resembled MIBC. We observed furthermore a correlation of histone methylation levels with pT stage (H3K9me1, H3K9me2, H3K9me3, H3K27me1, H3K27me3) and grade (H3K9me2, H3K9me3, H3K27me1) in NMIBC. H3K9me1, H3K9me3, H3K27me1 and H3K27me3 levels were also correlated with pT stage in MIBC. Histone modifications were not associated with recurrence-free or cancer-specific survival. CONCLUSIONS: Global histone H3K9 and H3K27 methylation levels are altered in BCa.
BACKGROUND: Epigenetic alterations, including histone modifications, play an important role during carcinogenesis. This study was designed to systematically investigate histone H3K9 and H3K27 methylation levels in bladder cancer (BCa) tissue. METHODS: A tissue microarray with urothelial BCa (150 non-muscle-invasive BCa, NMIBC; 121 muscle-invasive BCa, MIBC; 31 metastatic BCa, MET) and normal urothelium (29, CTRL) specimen was used to determine the global levels of H3K9 and H3K27 mono-, di- and tri-methylation. RESULTS: Global levels of H3K9 and H3K27 methylation were significantly higher in CTRL than in BCa, and levels in NMIBC were higher compared to MIBC. Histone methylation levels of MET resembled MIBC. We observed furthermore a correlation of histone methylation levels with pT stage (H3K9me1, H3K9me2, H3K9me3, H3K27me1, H3K27me3) and grade (H3K9me2, H3K9me3, H3K27me1) in NMIBC. H3K9me1, H3K9me3, H3K27me1 and H3K27me3 levels were also correlated with pT stage in MIBC. Histone modifications were not associated with recurrence-free or cancer-specific survival. CONCLUSIONS: Global histone H3K9 and H3K27 methylation levels are altered in BCa.
Authors: Brian T Joyce; Yinan Zheng; Drew Nannini; Zhou Zhang; Lei Liu; Tao Gao; Masha Kocherginsky; Robert Murphy; Hushan Yang; Chad J Achenbach; Lewis R Roberts; Mirjam Hoxha; Jincheng Shen; Pantel Vokonas; Joel Schwartz; Andrea Baccarelli; Lifang Hou Journal: Cancer Prev Res (Phila) Date: 2018-06-12
Authors: Anne Benard; Inès J Goossens-Beumer; Anneke Q van Hoesel; Wouter de Graaf; Hamed Horati; Hein Putter; Eliane C M Zeestraten; Cornelis J H van de Velde; Peter J K Kuppen Journal: BMC Cancer Date: 2014-07-22 Impact factor: 4.430
Authors: Aniek Janssen; Gregory A Breuer; Eva K Brinkman; Annelot I van der Meulen; Sean V Borden; Bas van Steensel; Ranjit S Bindra; Jeannine R LaRocque; Gary H Karpen Journal: Genes Dev Date: 2016-07-15 Impact factor: 11.361
Authors: Inês Faleiro; Ricardo Leão; Alexandra Binnie; Ramon Andrade de Mello; Ana-Teresa Maia; Pedro Castelo-Branco Journal: Oncotarget Date: 2017-02-14