Literature DB >> 2455636

Cloning and expression of cDNA for human vascular anticoagulant, a Ca2+-dependent phospholipid-binding protein.

I Maurer-Fogy1, C P Reutelingsperger, J Pieters, G Bodo, C Stratowa, R Hauptmann.   

Abstract

Based on sequence information from tryptic peptides an almost full-size cDNA coding for the human vascular anticoagulant was isolated from a placental cDNA library and sequenced. The coding region was cloned into an Escherichia coli expression vector and the protein expressed at high levels. The recombinant protein was purified and found to be indistinguishable from its natural counterpart in several biological assays.

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Year:  1988        PMID: 2455636     DOI: 10.1111/j.1432-1033.1988.tb14139.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN: 0014-2956


  21 in total

1.  Cloning and sequencing of rat plectin indicates a 466-kD polypeptide chain with a three-domain structure based on a central alpha-helical coiled coil.

Authors:  G Wiche; B Becker; K Luber; G Weitzer; M J Castañon; R Hauptmann; C Stratowa; M Stewart
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2.  Numerical classification of coding sequences.

Authors:  D W Collins; C C Liu; T H Jukes
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Journal:  J Biol Chem       Date:  2019-02-25       Impact factor: 5.157

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Authors:  J Römisch; E P Pâques
Journal:  Med Microbiol Immunol       Date:  1991       Impact factor: 3.402

5.  Annexin v - affinity assay - apoptosis detection system in granular cell ameloblastoma.

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6.  Annexin proteins PP4 and PP4-X. Comparative characterization of biological activities of placental and recombinant proteins.

Authors:  J Römisch; M Grote; K U Weithmann; N Heimburger; E Amann
Journal:  Biochem J       Date:  1990-11-15       Impact factor: 3.857

Review 7.  Novel antithrombotic drugs in development.

Authors:  M Verstraete; P Zoldhelyi
Journal:  Drugs       Date:  1995-06       Impact factor: 9.546

8.  Surface blebs on apoptotic cells are sites of enhanced procoagulant activity: implications for coagulation events and antigenic spread in systemic lupus erythematosus.

Authors:  L Casciola-Rosen; A Rosen; M Petri; M Schlissel
Journal:  Proc Natl Acad Sci U S A       Date:  1996-02-20       Impact factor: 11.205

Review 9.  Phosphatidylserine targeting for diagnosis and treatment of human diseases.

Authors:  Kristof Schutters; Chris Reutelingsperger
Journal:  Apoptosis       Date:  2010-09       Impact factor: 4.677

10.  Activation of prothrombin in the presence of human umbilical-vein endothelial cells.

Authors:  P Schoen; C Reutelingsperger; T Lindhout
Journal:  Biochem J       Date:  1992-02-01       Impact factor: 3.857

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