Pradyot Prakash1, Muktesh Khandare2, Mohan Kumar3, Rahul Khanna4, Gyan Prakash Singh5, Gopal Nath6, Anil Kumar Gulati6. 1. Assistant Professor, Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University , Varanasi-221005, India . 2. Senior Resident, Department of Pathology, Institute of Medical Sciences, Banaras Hindu University , Varanasi-221005, India . 3. Professor, Department of Pathology, Institute of Medical Sciences, Banaras Hindu University , Varanasi-221005, India . 4. Professor, Department of General Surgery, Institute of Medical Sciences, Banaras Hindu University , Varanasi-221005, India . 5. Associate Professor, Department of Community Medicine, Institute of Medical Sciences, Banaras Hindu University , Varanasi-221005, India . 6. Professor, Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University , Varanasi-221005, India .
Abstract
INTRODUCTION: Over-expression of p16(INK4a) has been reported in tissues of oral squamous cell carcinoma (SCC) associated with Human Papillomaviruses (HPVs). Immunohistochemical (IHC) detection of p16(INK4a) is an easy technique than molecular detection of HPVs, hence we investigated the presence of this protein in the most common pre-malignant and malignant oral lesions i.e. leukoplakia and SCC respectively. MATERIAL AND METHODS: We performed IHC detection of p16(INK4a) in sections of paraffin embedded formalin fixed tissues of leukoplakia with or without dysplasia (n= 21) and SCC lesions (n= 69) and correlated with various patterns of p16(INK4a) positivity with respect to histological diagnosis. RESULTS: In the present study, 71% cases of oral SCC cases were positive for p16(INK4a), of which the most common pattern was diffuse nuclear and cytoplasmic staining. Among the cases with leukoplakia, 57.1% were positive for overexpression of p16(INK4a), wherein diffuse and sporadic pattern was observed among 23.8 percent each. CONCLUSION: In the present study, significant number of oral SCC cases observed overexpressing p16(INK4a) . However HPV DNA detection based studies are needed to validate the utility of IHC detection of p16(INK4a) as a surrogate marker for HPV associated oral SCC.
INTRODUCTION: Over-expression of p16(INK4a) has been reported in tissues of oral squamous cell carcinoma (SCC) associated with Human Papillomaviruses (HPVs). Immunohistochemical (IHC) detection of p16(INK4a) is an easy technique than molecular detection of HPVs, hence we investigated the presence of this protein in the most common pre-malignant and malignant oral lesions i.e. leukoplakia and SCC respectively. MATERIAL AND METHODS: We performed IHC detection of p16(INK4a) in sections of paraffin embedded formalin fixed tissues of leukoplakia with or without dysplasia (n= 21) and SCC lesions (n= 69) and correlated with various patterns of p16(INK4a) positivity with respect to histological diagnosis. RESULTS: In the present study, 71% cases of oral SCC cases were positive for p16(INK4a), of which the most common pattern was diffuse nuclear and cytoplasmic staining. Among the cases with leukoplakia, 57.1% were positive for overexpression of p16(INK4a), wherein diffuse and sporadic pattern was observed among 23.8 percent each. CONCLUSION: In the present study, significant number of oral SCC cases observed overexpressing p16(INK4a) . However HPV DNA detection based studies are needed to validate the utility of IHC detection of p16(INK4a) as a surrogate marker for HPV associated oral SCC.
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