Literature DB >> 24549663

Kinetic analysis of in vitro pre-mRNA splicing in HeLa nuclear extract.

William F Mueller1, Klemens J Hertel.   

Abstract

Kinetic analysis of in vitro splicing is a valuable technique for understanding splicing regulation. It allows the determination of specific contributions from functional elements for the efficient removal of introns. This chapter will describe the rationale and approach employed to use kinetic analysis to evaluate an in vitro splicing reaction using radiolabeled pre-mRNA incubated in splicing-competent HeLa nuclear extract (NE).

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Year:  2014        PMID： 24549663      PMCID： PMC4688299          DOI： 10.1007/978-1-62703-980-2_12

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  19 in total

1.  Commitment to splice site pairing coincides with A complex formation.

Authors:  Sharlene R Lim; Klemens J Hertel
Journal:  Mol Cell       Date:  2004-08-13       Impact factor: 17.970

Review 2.  Multiple links between transcription and splicing.

Authors:  Alberto R Kornblihtt; Manuel de la Mata; Juan Pablo Fededa; Manuel J Munoz; Guadalupe Nogues
Journal:  RNA       Date:  2004-10       Impact factor: 4.942

3.  The architecture of pre-mRNAs affects mechanisms of splice-site pairing.

Authors:  Kristi L Fox-Walsh; Yimeng Dou; Bianca J Lam; She-Pin Hung; Pierre F Baldi; Klemens J Hertel
Journal:  Proc Natl Acad Sci U S A       Date:  2005-10-31       Impact factor: 11.205

4.  Analyzing mechanisms of alternative pre-mRNA splicing using in vitro splicing assays.

Authors:  Martin J Hicks; Bianca J Lam; Klemens J Hertel
Journal:  Methods       Date:  2005-12       Impact factor: 3.608

5.  Modulation of 5' splice site choice in pre-messenger RNA by two distinct steps.

Authors:  W Y Tarn; J A Steitz
Journal:  Proc Natl Acad Sci U S A       Date:  1995-03-28       Impact factor: 11.205

6.  SR proteins can compensate for the loss of U1 snRNP functions in vitro.

Authors:  W Y Tarn; J A Steitz
Journal:  Genes Dev       Date:  1994-11-15       Impact factor: 11.361

7.  Serine-arginine (SR)-rich splicing factors have an exon-independent function in pre-mRNA splicing.

Authors:  K J Hertel; T Maniatis
Journal:  Proc Natl Acad Sci U S A       Date:  1999-03-16       Impact factor: 11.205

8.  Enhancer-dependent 5'-splice site control of fruitless pre-mRNA splicing.

Authors:  Bianca J Lam; Arati Bakshi; Fatma Y Ekinci; Jenny Webb; Brenton R Graveley; Klemens J Hertel
Journal:  J Biol Chem       Date:  2003-03-19       Impact factor: 5.157

9.  Normal and mutant human beta-globin pre-mRNAs are faithfully and efficiently spliced in vitro.

Authors:  A R Krainer; T Maniatis; B Ruskin; M R Green
Journal:  Cell       Date:  1984-04       Impact factor: 41.582

10.  Purification and electron microscopic visualization of functional human spliceosomes.

Authors:  Zhaolan Zhou; Jeonggu Sim; Jack Griffith; Robin Reed
Journal:  Proc Natl Acad Sci U S A       Date:  2002-09-05       Impact factor: 11.205
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