| Literature DB >> 2454622 |
N V Bulychev, D M Graĭfer, G G Karpova, A V Lebedev.
Abstract
A method of rapid preparative isolation of Phe-tRNA(Phe) (E. coli) of almost 100% purity (1800 pmoles per 1 OE260) is developed. In includes three successive chromatographies of total tRNA on C18-modified Lichrosphere Si-1000 in acetonitrile gradients. Other individual tRNAs can be isolated by the same approach.Entities:
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Year: 1988 PMID： 2454622
Source DB: PubMed Journal: Bioorg Khim ISSN： 0132-3423