Literature DB >> 2453682

A single 66-kilodalton polypeptide processed from the human immunodeficiency virus type 2 pol polyprotein in Escherichia coli displays reverse transcriptase activity.

S F Le Grice1, R Zehnle, J Mous.   

Abstract

We have cloned the entire pol gene of human immunodeficiency virus type 2 into a high-level Escherichia coli expression system. Induction of cultures containing the recombinant plasmid, p2RTL1, leads to rapid accumulation of polypeptides of 66, 54, and 34 kilodaltons. We have designated the larger polypeptides reverse transcriptase, and we have designated the smaller polypeptide endonuclease. Purification of reverse transcriptase via ion-exchange and affinity chromatography yields the 66-kilodalton polypeptide, with which reverse transcriptase activity is associated. Purified enzyme furthermore displays a higher apparent molecular weight than its counterpart from human immunodeficiency virus type 1.

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Year:  1988        PMID： 2453682      PMCID： PMC253416     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  17 in total

1.  Characterization of highly immunogenic p66/p51 as the reverse transcriptase of HTLV-III/LAV.

Authors:  F di Marzo Veronese; T D Copeland; A L DeVico; R Rahman; S Oroszlan; R C Gallo; M G Sarngadharan
Journal:  Science       Date:  1986-03-14       Impact factor: 47.728

2.  Expression of reverse transcriptase activity of human T-lymphotropic virus type III (HTLV-III/LAV) in Escherichia coli.

Authors:  N Tanese; J Sodroski; W A Haseltine; S P Goff
Journal:  J Virol       Date:  1986-09       Impact factor: 5.103

3.  Isolation of a new human retrovirus from West African patients with AIDS.

Authors:  F Clavel; D Guétard; F Brun-Vézinet; S Chamaret; M A Rey; M O Santos-Ferreira; A G Laurent; C Dauguet; C Katlama; C Rouzioux
Journal:  Science       Date:  1986-07-18       Impact factor: 47.728

4.  RNase H activity associated with bacterially expressed reverse transcriptase of human T-cell lymphotropic virus III/lymphadenopathy-associated virus.

Authors:  J Hansen; T Schulze; K Moelling
Journal:  J Biol Chem       Date:  1987-09-15       Impact factor: 5.157

5.  Expression of biologically active human T-cell lymphotropic virus type III reverse transcriptase in Bacillus subtilis.

Authors:  S F Le Grice; V Beuck; J Mous
Journal:  Gene       Date:  1987       Impact factor: 3.688

6.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

7.  3'-Azido-3'-deoxythymidine (BW A509U): an antiviral agent that inhibits the infectivity and cytopathic effect of human T-lymphotropic virus type III/lymphadenopathy-associated virus in vitro.

Authors:  H Mitsuya; K J Weinhold; P A Furman; M H St Clair; S N Lehrman; R C Gallo; D Bolognesi; D W Barry; S Broder
Journal:  Proc Natl Acad Sci U S A       Date:  1985-10       Impact factor: 11.205

8.  Efficient utilization of Escherichia coli transcriptional signals in Bacillus subtilis.

Authors:  U Peschke; V Beuck; H Bujard; R Gentz; S Le Grice
Journal:  J Mol Biol       Date:  1985-12-05       Impact factor: 5.469

9.  Structural characterization of reverse transcriptase and endonuclease polypeptides of the acquired immunodeficiency syndrome retrovirus.

Authors:  M M Lightfoote; J E Coligan; T M Folks; A S Fauci; M A Martin; S Venkatesan
Journal:  J Virol       Date:  1986-11       Impact factor: 5.103

10.  A novel in vitro transcription-translation system: accurate and efficient synthesis of single proteins from cloned DNA sequences.

Authors:  D Stueber; I Ibrahimi; D Cutler; B Dobberstein; H Bujard
Journal:  EMBO J       Date:  1984-12-20       Impact factor: 11.598
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  9 in total

1.  Cassette mutagenesis of the reverse transcriptase of human immunodeficiency virus type 1.

Authors:  P L Boyer; A L Ferris; S H Hughes
Journal:  J Virol       Date:  1992-02       Impact factor: 5.103

2.  Purification and characterization of recombinant equine infectious anemia virus reverse transcriptase.

Authors:  S F Le Grice; M Panin; R C Kalayjian; N J Richter; G Keith; J L Darlix; S L Payne
Journal:  J Virol       Date:  1991-12       Impact factor: 5.103

3.  Linker insertion mutagenesis of the human immunodeficiency virus reverse transcriptase expressed in bacteria: definition of the minimal polymerase domain.

Authors:  V R Prasad; S P Goff
Journal:  Proc Natl Acad Sci U S A       Date:  1989-05       Impact factor: 11.205

4.  Nonnucleoside reverse transcriptase inhibitors that potently and specifically block human immunodeficiency virus type 1 replication.

Authors:  D L Romero; M Busso; C K Tan; F Reusser; J R Palmer; S M Poppe; P A Aristoff; K M Downey; A G So; L Resnick
Journal:  Proc Natl Acad Sci U S A       Date:  1991-10-01       Impact factor: 11.205

5.  Reverse transcriptase of mouse mammary tumour virus: expression in bacteria, purification and biochemical characterization.

Authors:  R Taube; S Loya; O Avidan; M Perach; A Hizi
Journal:  Biochem J       Date:  1998-02-01       Impact factor: 3.857

6.  Initiation of (-) strand DNA synthesis from tRNA(3Lys) on lentiviral RNAs: implications of specific HIV-1 RNA-tRNA(3Lys) interactions inhibiting primer utilization by retroviral reverse transcriptases.

Authors:  E J Arts; S R Stetor; X Li; J W Rausch; K J Howard; B Ehresmann; T W North; B M Wöhrl; R S Goody; M A Wainberg; S F Grice
Journal:  Proc Natl Acad Sci U S A       Date:  1996-09-17       Impact factor: 11.205

7.  Mutational analysis of the fingers domain of human immunodeficiency virus type 1 reverse transcriptase.

Authors:  P L Boyer; A L Ferris; S H Hughes
Journal:  J Virol       Date:  1992-12       Impact factor: 5.103

8.  Human immunodeficiency virus type 2 reverse transcriptase activity in model systems that mimic steps in reverse transcription.

Authors:  Klara Post; Jianhui Guo; Kathryn J Howard; Michael D Powell; Jennifer T Miller; Amnon Hizi; Stuart F J Le Grice; Judith G Levin
Journal:  J Virol       Date:  2003-07       Impact factor: 5.103

9.  Domain structure of the human immunodeficiency virus reverse transcriptase.

Authors:  H Lederer; O Schatz; R May; H Crespi; J L Darlix; S F Le Grice; H Heumann
Journal:  EMBO J       Date:  1992-03       Impact factor: 11.598
  9 in total

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