Literature DB >> 1719238

Purification and characterization of recombinant equine infectious anemia virus reverse transcriptase.

S F Le Grice1, M Panin, R C Kalayjian, N J Richter, G Keith, J L Darlix, S L Payne.   

Abstract

A 1.67-kb segment of the equine infectious anemia virus pol gene, encoding a 66-kDa reverse transcriptase (RT), was cloned and expressed in Escherichia coli. Recombinant RT, purified by a combination of metal chelate affinity chromatography and ion-exchange chromatography, displays both RNA-dependent DNA polymerase and RNase H activity. The affinity of purified RT for its replication primer, tRNA(3Lys) was equivalent to that observed for human immunodeficiency virus RT. Our data suggest that an additional domain between RT-RNase H and integrase on the equine infectious anemia virus pol open reading frame is not an integral component of the RT polypeptide.

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Year:  1991        PMID： 1719238      PMCID： PMC250816     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  35 in total

1.  Computer analysis of retroviral pol genes: assignment of enzymatic functions to specific sequences and homologies with nonviral enzymes.

Authors:  M S Johnson; M A McClure; D F Feng; J Gray; R F Doolittle
Journal:  Proc Natl Acad Sci U S A       Date:  1986-10       Impact factor: 11.205

2.  Standardized and simplified nomenclature for proteins common to all retroviruses.

Authors:  J Leis; D Baltimore; J M Bishop; J Coffin; E Fleissner; S P Goff; S Oroszlan; H Robinson; A M Skalka; H M Temin
Journal:  J Virol       Date:  1988-05       Impact factor: 5.103

3.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Authors:  R K Saiki; D H Gelfand; S Stoffel; S J Scharf; R Higuchi; G T Horn; K B Mullis; H A Erlich
Journal:  Science       Date:  1988-01-29       Impact factor: 47.728

4.  Human immunodeficiency virus reverse transcriptase expressed in transformed yeast cells. Biochemical properties and interactions with bovine tRNALys.

Authors:  M L Sallafranque-Andreola; D Robert; P J Barr; M Fournier; S Litvak; L Sarih-Cottin; L Tarrago-Litvak
Journal:  Eur J Biochem       Date:  1989-09-15

5.  Rapid emergence of novel antigenic and genetic variants of equine infectious anemia virus during persistent infection.

Authors:  O Salinovich; S L Payne; R C Montelaro; K A Hussain; C J Issel; K L Schnorr
Journal:  J Virol       Date:  1986-01       Impact factor: 5.103

6.  Isolation of an HTLV-III-related retrovirus from macaques with simian AIDS and its possible origin in asymptomatic mangabeys.

Authors:  M Murphey-Corb; L N Martin; S R Rangan; G B Baskin; B J Gormus; R H Wolf; W A Andes; M West; R C Montelaro
Journal:  Nature       Date:  1986 May 22-28       Impact factor: 49.962

7.  Cordycepin analogues of 2',5'-oligoadenylate inhibit human immunodeficiency virus infection via inhibition of reverse transcriptase.

Authors:  W E Müller; B E Weiler; R Charubala; W Pfleiderer; L Leserman; R W Sobol; R J Suhadolnik; H C Schröder
Journal:  Biochemistry       Date:  1991-02-26       Impact factor: 3.162

8.  Molecular cloning of feline immunodeficiency virus.

Authors:  R A Olmsted; A K Barnes; J K Yamamoto; V M Hirsch; R H Purcell; P R Johnson
Journal:  Proc Natl Acad Sci U S A       Date:  1989-04       Impact factor: 11.205

9.  HIV-1 reverse transcriptase specifically interacts with the anticodon domain of its cognate primer tRNA.

Authors:  C Barat; V Lullien; O Schatz; G Keith; M T Nugeyre; F Grüninger-Leitch; F Barré-Sinoussi; S F LeGrice; J L Darlix
Journal:  EMBO J       Date:  1989-11       Impact factor: 11.598

10.  Subregions of a conserved part of the HIV gp41 transmembrane protein are differentially recognized by antibodies of infected individuals.

Authors:  U Certa; W Bannwarth; D Stüber; R Gentz; M Lanzer; S Le Grice; F Guillot; I Wendler; G Hunsmann; H Bujard
Journal:  EMBO J       Date:  1986-11       Impact factor: 11.598
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  3 in total

1.  Characterization of equine infectious anemia virus dUTPase: growth properties of a dUTPase-deficient mutant.

Authors:  D S Threadgill; W K Steagall; M T Flaherty; F J Fuller; S T Perry; K E Rushlow; S F Le Grice; S L Payne
Journal:  J Virol       Date:  1993-05       Impact factor: 5.103

2.  Reverse transcriptase of mouse mammary tumour virus: expression in bacteria, purification and biochemical characterization.

Authors:  R Taube; S Loya; O Avidan; M Perach; A Hizi
Journal:  Biochem J       Date:  1998-02-01       Impact factor: 3.857

3.  Initiation of (-) strand DNA synthesis from tRNA(3Lys) on lentiviral RNAs: implications of specific HIV-1 RNA-tRNA(3Lys) interactions inhibiting primer utilization by retroviral reverse transcriptases.

Authors:  E J Arts; S R Stetor; X Li; J W Rausch; K J Howard; B Ehresmann; T W North; B M Wöhrl; R S Goody; M A Wainberg; S F Grice
Journal:  Proc Natl Acad Sci U S A       Date:  1996-09-17       Impact factor: 11.205
  3 in total

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