Jong-Suk Yoon1, Sang-Hwa Lee1, Hyun-Joong Yoon2. 1. Department of Oral and Maxillofacial Surgery, Yeouido St. Mary's Hospital, Catholic University of Korea, Seoul, Republic of Korea. 2. Department of Oral and Maxillofacial Surgery, Yeouido St. Mary's Hospital, Catholic University of Korea, Seoul, Republic of Korea. Electronic address: omfsyhj@catholic.ac.kr.
Abstract
PURPOSE: The purpose of this study was to investigate the influence of platelet-rich fibrin (PRF) on angiogenesis and osteogenesis in guided bone regeneration (GBR) using xenogenic bone in rabbit cranial defects. MATERIALS AND METHODS: In each rabbit, 2 circular bone defects, one on either side of the midline, were prepared using a reamer drill. Each of the experimental sites received bovine bone with PRF, and each of the control sites received bovine bone alone. The animals were sacrificed at 1 week (n = 4), 2 weeks (n = 3) and 4 weeks (n = 3). Biopsy samples were examined histomorphometrically by light microscopy, and expression of vascular endothelial growth factor (VEGF) was determined by immunohistochemical staining. RESULTS: At all experimental time points, immunostaining intensity for VEGF was consistently higher in the experimental group than in the control group. However, the differences between the control group and the experimental group were not statistically significant in the histomorphometrical and immunohistochemical examinations. CONCLUSIONS: The results of this study suggest that PRF may increase the number of marrow cells. However, PRF along with xenogenic bone substitutes does not show a significant effect on bony regeneration. Further large-scale studies are needed to confirm our results.
PURPOSE: The purpose of this study was to investigate the influence of platelet-rich fibrin (PRF) on angiogenesis and osteogenesis in guided bone regeneration (GBR) using xenogenic bone in rabbit cranial defects. MATERIALS AND METHODS: In each rabbit, 2 circular bone defects, one on either side of the midline, were prepared using a reamer drill. Each of the experimental sites received bovine bone with PRF, and each of the control sites received bovine bone alone. The animals were sacrificed at 1 week (n = 4), 2 weeks (n = 3) and 4 weeks (n = 3). Biopsy samples were examined histomorphometrically by light microscopy, and expression of vascular endothelial growth factor (VEGF) was determined by immunohistochemical staining. RESULTS: At all experimental time points, immunostaining intensity for VEGF was consistently higher in the experimental group than in the control group. However, the differences between the control group and the experimental group were not statistically significant in the histomorphometrical and immunohistochemical examinations. CONCLUSIONS: The results of this study suggest that PRF may increase the number of marrow cells. However, PRF along with xenogenic bone substitutes does not show a significant effect on bony regeneration. Further large-scale studies are needed to confirm our results.
Authors: Fernanda Faot; Sanne Deprez; Katleen Vandamme; Germana V Camargos; Nelson Pinto; Jasper Wouters; Joost van den Oord; Marc Quirynen; Joke Duyck Journal: Sci Rep Date: 2017-04-12 Impact factor: 4.379
Authors: Sebastian Blatt; Daniel G E Thiem; Solomiya Kyyak; Andreas Pabst; Bilal Al-Nawas; Peer W Kämmerer Journal: Front Bioeng Biotechnol Date: 2021-03-16