Literature DB >> 2452787

Studies of a human lambda-chain epitope related to a complementarity-determining region.

H S Kim1, H F Deutsch.   

Abstract

A tryptic non-adecapeptide representing the 24-42 sequence of the MCG lambda-type Bence-Jones protein, and which contains its entire complementarity-determining region-1, was isolated. The peptide was utilized in preparing an affinity column that was used to isolate an antibody having the reactivity of a previously employed idiotypic antibody to MCG. This antibody preparation, as well as 13 monoclonal mouse antibodies to human lambda-chains, was employed in an enzyme-linked immunoassay to detect other Bence-Jones proteins with this serologic specificity. The results obtained with two of the monoclonal antibodies suggest that the epitope in question is a noncontiguous one.

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Year:  1988        PMID： 2452787      PMCID： PMC1454789     

Source DB:  PubMed          Journal:  Immunology        ISSN： 0019-2805            Impact factor:   7.397


  17 in total

1.  A simplified method for cyanogen bromide activation of agarose for affinity chromatography.

Authors:  S C March; I Parikh; P Cuatrecasas
Journal:  Anal Biochem       Date:  1974-07       Impact factor: 3.365

2.  The reactivity of anti-peptide antibodies is a function of the atomic mobility of sites in a protein.

Authors:  J A Tainer; E D Getzoff; H Alexander; R A Houghten; A J Olson; R A Lerner; W A Hendrickson
Journal:  Nature       Date:  1984 Nov 8-14       Impact factor: 49.962

3.  Synthetic idiotypes: the third hypervariable region of murine anti-dextran antibodies.

Authors:  S McMillan; M V Seiden; R A Houghten; B Clevinger; J M Davie; R A Lerner
Journal:  Cell       Date:  1983-12       Impact factor: 41.582

4.  Generation of protein-reactive antibodies by short peptides is an event of high frequency: implications for the structural basis of immune recognition.

Authors:  H L Niman; R A Houghten; L E Walker; R A Reisfeld; I A Wilson; J M Hogle; R A Lerner
Journal:  Proc Natl Acad Sci U S A       Date:  1983-08       Impact factor: 11.205

5.  Antibodies with specificities to preselected protein regions evoked by free synthetic peptides representing protein antigenic sites or other surface locations: demonstration with myoglobin.

Authors:  D R Young; H E Schmitz; M Z Atassi
Journal:  Mol Immunol       Date:  1983-05       Impact factor: 4.407

6.  Immunogenic structure of the influenza virus hemagglutinin.

Authors:  N Green; H Alexander; A Olson; S Alexander; T M Shinnick; J G Sutcliffe; R A Lerner
Journal:  Cell       Date:  1982-03       Impact factor: 41.582

7.  Unexpected similarities in the crystal structures of the Mcg light-chain dimer and its hybrid with the Weir protein.

Authors:  K R Ely; M K Wood; S S Rajan; J M Hodsdon; E E Abola; H F Deutsch; A B Edmundson
Journal:  Mol Immunol       Date:  1985-02       Impact factor: 4.407

8.  Primary structure of a human lambda-chain (Weir) of the Mcg type.

Authors:  J R Jabusch; H F Deutsch
Journal:  Mol Immunol       Date:  1982-07       Impact factor: 4.407

9.  Variable-region sequences of five human lambda-chain proteins reacting with an idiotypic antibody to the MCG Bence-Jones protein.

Authors:  D Zhu; H S Kim; H F Deutsch
Journal:  Mol Immunol       Date:  1983-10       Impact factor: 4.407

10.  N-(beta-Iodoethyl)trifluoroacetamide: a new reagent for the aminoethylation of thio groups in proteins.

Authors:  W E Schwartz; P K Smith; G P Royer
Journal:  Anal Biochem       Date:  1980-07-15       Impact factor: 3.365
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  1 in total

1.  Variable-region sequence homologies of human kappa-chains with idiotypic serologic similarities.

Authors:  H S Kim; H F Deutsch
Journal:  Immunology       Date:  1988-08       Impact factor: 7.397
  1 in total

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