BACKGROUND/AIMS: The maximum lifespan of the naked mole rat is over 28.3 years, which exceeds that of any other rodent species, suggesting that age-related changes in its body composition and functionality are either attenuated or delayed in this extraordinarily long-lived species. However, the mechanisms underlying the aging process in this species are poorly understood. In this study, we investigated whether long-lived naked mole rats display more autophagic activity than short-lived mice. METHODS: Hepatic stellate cells isolated from naked mole rats were treated with 50 nM rapamycin or 20 mM 3-methyladenine (3-MA) for 12 or 24 h. Expression of the autophagy marker proteins LC3-II and beclin 1 was measured with western blotting and immunohistochemistry. The induction of apoptosis was analyzed by flow cytometry. RESULTS: Our results demonstrate that one-day-old naked mole rats have higher levels of autophagy than one-day-old short-lived C57BL/6 mice, and that both adult naked mole rats (eight months old) and adult C57BL/6 mice (eight weeks old) have high basal levels of autophagy, which may be an important mechanism inhibiting aging and reducing the risk of age-related diseases. CONCLUSION: Here, we report that autophagy facilitated the survival of hepatic stellate cells from the naked mole rat, and that treatment with 3-MA or rapamycin increased the ratio of apoptotic cells to normal hepatic stellate cells.
BACKGROUND/AIMS: The maximum lifespan of the naked mole rat is over 28.3 years, which exceeds that of any other rodent species, suggesting that age-related changes in its body composition and functionality are either attenuated or delayed in this extraordinarily long-lived species. However, the mechanisms underlying the aging process in this species are poorly understood. In this study, we investigated whether long-lived naked mole rats display more autophagic activity than short-lived mice. METHODS: Hepatic stellate cells isolated from naked mole rats were treated with 50 nM rapamycin or 20 mM 3-methyladenine (3-MA) for 12 or 24 h. Expression of the autophagy marker proteins LC3-II and beclin 1 was measured with western blotting and immunohistochemistry. The induction of apoptosis was analyzed by flow cytometry. RESULTS: Our results demonstrate that one-day-old naked mole rats have higher levels of autophagy than one-day-old short-lived C57BL/6 mice, and that both adult naked mole rats (eight months old) and adult C57BL/6 mice (eight weeks old) have high basal levels of autophagy, which may be an important mechanism inhibiting aging and reducing the risk of age-related diseases. CONCLUSION: Here, we report that autophagy facilitated the survival of hepatic stellate cells from the naked mole rat, and that treatment with 3-MA or rapamycin increased the ratio of apoptotic cells to normal hepatic stellate cells.
Authors: Yang Zhao; Alexander Tyshkovskiy; Daniel Muñoz-Espín; Xiao Tian; Manuel Serrano; Joao Pedro de Magalhaes; Eviatar Nevo; Vadim N Gladyshev; Andrei Seluanov; Vera Gorbunova Journal: Proc Natl Acad Sci U S A Date: 2018-02-05 Impact factor: 11.205
Authors: David F Carrageta; Bárbara Guerra-Carvalho; Maria Angélica Spadella; Marc Yeste; Pedro F Oliveira; Marco G Alves Journal: Rev Endocr Metab Disord Date: 2022-05-23 Impact factor: 6.514
Authors: Judy C Triplett; Aaron M Swomley; Jessime Kirk; Kelly M Grimes; Kaitilyn N Lewis; Miranda E Orr; Karl A Rodriguez; Jian Cai; Jon B Klein; Rochelle Buffenstein; D Allan Butterfield Journal: Neurochem Res Date: 2016-03-02 Impact factor: 3.996
Authors: Judy C Triplett; Antonella Tramutola; Aaron Swomley; Jessime Kirk; Kelly Grimes; Kaitilyn Lewis; Miranda Orr; Karl Rodriguez; Jian Cai; Jon B Klein; Marzia Perluigi; Rochelle Buffenstein; D Allan Butterfield Journal: Biochim Biophys Acta Date: 2015-08-04
Authors: Susanne Holtze; Ekaterina Gorshkova; Stan Braude; Alessandro Cellerino; Philip Dammann; Thomas B Hildebrandt; Andreas Hoeflich; Steve Hoffmann; Philipp Koch; Eva Terzibasi Tozzini; Maxim Skulachev; Vladimir P Skulachev; Arne Sahm Journal: Front Mol Biosci Date: 2021-05-17