Literature DB >> 24522395

A simple method to estimate the average localization precision of a single-molecule localization microscopy experiment.

Ulrike Endesfelder1, Sebastian Malkusch, Franziska Fricke, Mike Heilemann.   

Abstract

The localization precision is a crucial and important parameter for single-molecule localization microscopy (SMLM) and directly influences the achievable spatial resolution. It primarily depends on experimental imaging conditions and the registration potency of the algorithm used. We propose a new and simple routine to estimate the average experimental localization precision in SMLM, based on the nearest neighbor analysis. By exploring different experimental and simulated targets, we show that this approach can be generally used for any 2D or 3D SMLM data and that reliable values for the localization precision σ SMLM are obtained. Knowing σ SMLM is a prerequisite for consistent visualization or any quantitative structural analysis, e.g., cluster analysis or colocalization studies.

Mesh：

Year:  2014        PMID： 24522395     DOI： 10.1007/s00418-014-1192-3

Source DB:  PubMed          Journal:  Histochem Cell Biol        ISSN： 0948-6143            Impact factor:   4.304


  35 in total

1.  Imaging and tracking of single GFP molecules in solution.

Authors:  U Kubitscheck; O Kückmann; T Kues; R Peters
Journal:  Biophys J       Date:  2000-04       Impact factor: 4.033

2.  Quantitative comparison of algorithms for tracking single fluorescent particles.

Authors:  M K Cheezum; W F Walker; W H Guilford
Journal:  Biophys J       Date:  2001-10       Impact factor: 4.033

3.  The influence of movement on the localization precision of sub-resolution particles in fluorescence microscopy.

Authors:  Hendrik Deschout; Kristiaan Neyts; Kevin Braeckmans
Journal:  J Biophotonics       Date:  2011-11-15       Impact factor: 3.207

4.  Super-resolution imaging visualizes the eightfold symmetry of gp210 proteins around the nuclear pore complex and resolves the central channel with nanometer resolution.

Authors:  Anna Löschberger; Sebastian van de Linde; Marie-Christine Dabauvalle; Bernd Rieger; Mike Heilemann; Georg Krohne; Markus Sauer
Journal:  J Cell Sci       Date:  2012-02-01       Impact factor: 5.285

5.  Minimum variance unbiased subpixel centroid estimation of point image limited by photon shot noise.

Authors:  Hui Jia; Jiankun Yang; Xiujian Li
Journal:  J Opt Soc Am A Opt Image Sci Vis       Date:  2010-09-01       Impact factor: 2.129

6.  Real-time computation of subdiffraction-resolution fluorescence images.

Authors:  S Wolter; M Schüttpelz; M Tscherepanow; S VAN DE Linde; M Heilemann; M Sauer
Journal:  J Microsc       Date:  2010-01       Impact factor: 1.758

7.  Measuring localization performance of super-resolution algorithms on very active samples.

Authors:  Steve Wolter; Ulrike Endesfelder; Sebastian van de Linde; Mike Heilemann; Markus Sauer
Journal:  Opt Express       Date:  2011-04-11       Impact factor: 3.894

8.  Limit of the Accuracy of Parameter Estimation for Moving Single Molecules Imaged by Fluorescence Microscopy.

Authors:  Yau Wong; Zhiping Lin; Raimund J Ober
Journal:  IEEE Trans Signal Process       Date:  2011       Impact factor: 4.931

9.  Chemically induced photoswitching of fluorescent probes--a general concept for super-resolution microscopy.

Authors:  Ulrike Endesfelder; Sebastian Malkusch; Benjamin Flottmann; Justine Mondry; Piotr Liguzinski; Peter J Verveer; Mike Heilemann
Journal:  Molecules       Date:  2011-04-13       Impact factor: 4.411

10.  Resolution doubling in 3D-STORM imaging through improved buffers.

Authors:  Nicolas Olivier; Debora Keller; Pierre Gönczy; Suliana Manley
Journal:  PLoS One       Date:  2013-07-17       Impact factor: 3.240
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  69 in total

Review 1.  The Histochemistry and Cell Biology pandect: the year 2014 in review.

Authors:  Douglas J Taatjes; Jürgen Roth
Journal:  Histochem Cell Biol       Date:  2015-03-06       Impact factor: 4.304

2.  Super-resolution microscopy with DNA-PAINT.

Authors:  Joerg Schnitzbauer; Maximilian T Strauss; Thomas Schlichthaerle; Florian Schueder; Ralf Jungmann
Journal:  Nat Protoc       Date:  2017-05-18       Impact factor: 13.491

3.  Art and artifacts in single-molecule localization microscopy: beyond attractive images.

Authors:  Ulrike Endesfelder; Mike Heilemann
Journal:  Nat Methods       Date:  2014-03       Impact factor: 28.547

4.  In this special issue. In focus: Single-molecule super-resolution microscopy.

Authors:  Jürgen Roth; Mike Heilemann
Journal:  Histochem Cell Biol       Date:  2014-05-21       Impact factor: 4.304

5.  SuReSim: simulating localization microscopy experiments from ground truth models.

Authors:  Varun Venkataramani; Frank Herrmannsdörfer; Mike Heilemann; Thomas Kuner
Journal:  Nat Methods       Date:  2016-02-29       Impact factor: 28.547

6.  Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy.

Authors:  Cécile Leduc; Audrey Salles; Spencer L Shorte; Sandrine Etienne-Manneville
Journal:  J Vis Exp       Date:  2018-03-06       Impact factor: 1.355

7.  A transient pool of nuclear F-actin at mitotic exit controls chromatin organization.

Authors:  Christian Baarlink; Matthias Plessner; Alice Sherrard; Kohtaro Morita; Shinji Misu; David Virant; Eva-Maria Kleinschnitz; Robert Harniman; Dominic Alibhai; Stefan Baumeister; Kei Miyamoto; Ulrike Endesfelder; Abderrahmane Kaidi; Robert Grosse
Journal:  Nat Cell Biol       Date:  2017-11-13       Impact factor: 28.824

Review 8.  Single-molecule localization to study cytoskeletal structures, membrane complexes, and mechanosensors.

Authors:  R Magrassi; S Scalisi; F Cella Zanacchi
Journal:  Biophys Rev       Date:  2019-09-16

9.  Nanoscale co-organization and coactivation of AMPAR, NMDAR, and mGluR at excitatory synapses.

Authors:  Julia Goncalves; Tomas M Bartol; Côme Camus; Florian Levet; Ana Paula Menegolla; Terrence J Sejnowski; Jean-Baptiste Sibarita; Michel Vivaudou; Daniel Choquet; Eric Hosy
Journal:  Proc Natl Acad Sci U S A       Date:  2020-06-08       Impact factor: 11.205

10.  Live-cell super-resolved PAINT imaging of piconewton cellular traction forces.

Authors:  Joshua M Brockman; Hanquan Su; Aaron T Blanchard; Yuxin Duan; Travis Meyer; M Edward Quach; Roxanne Glazier; Alisina Bazrafshan; Rachel L Bender; Anna V Kellner; Hiroaki Ogasawara; Rong Ma; Florian Schueder; Brian G Petrich; Ralf Jungmann; Renhao Li; Alexa L Mattheyses; Yonggang Ke; Khalid Salaita
Journal:  Nat Methods       Date:  2020-09-14       Impact factor: 28.547
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