Richard Stange1,2, Hacer Sahin3,4, Britta Wieskötter3, Thorsten Persigehl5,6, Janine Ring5, Christoph Bremer5,7, Michael J Raschke3, Volker Vieth5. 1. Department of Trauma, Hand and Reconstructive Surgery, University Hospital Münster, Münster, Germany. richard.stange@ukmuenster.de. 2. Department of Trauma, Hand and Reconstructive Surgery, Institute of Experimental Musculoskeletal Medicine (IEMM), University Hospital Münster, Waldeyerstr. 1, 48161, Münster, Germany. richard.stange@ukmuenster.de. 3. Department of Trauma, Hand and Reconstructive Surgery, University Hospital Münster, Münster, Germany. 4. Medical Department III, University Hospital Aachen, RWTH Aachen, Aachen, Germany. 5. Department of Clinical Radiology, University Hospital Münster, Münster, Germany. 6. Department of Radiology, University Hospital Cologne, Cologne, Germany. 7. Department of Radiology, St. Franziskus Hospital Münster, Münster, Germany.
Abstract
PURPOSE: Recent in vivo studies were able to show the impairing effect of neoangiogenesis in degenerative tendon diseases. Clinical in vivo monitoring of angiogenesis in injured tendons therefore seems to be crucial for an accurate therapeutic approach. The aim of this study was to develop a novel magnetic resonance imaging (MRI)-based technique for observing angiogenesis during tendon healing in vivo. METHODS: Tendinopathy was induced by an in situ freezing model of rat patellar tendon and monitored after 7, 14, and 28 days. Animals were randomly divided into an imaging and immunohistochemical group. MRI with a 'blood pool' contrast agent was used to determine neoangiogenesis during tendon healing. MRI was compared to histochemical staining and quantification of blood vessels in injured and native tendons. RESULTS: MRI data revealed a peak in changes in the transverse relaxation rate (ΔR 2*), which is proportional to relative blood volume, 7 days after surgery and decrease until day 28. Histological microvessel density and vascular endothelial growth factor synthesis were also most evident at day 7 and decreased over time. CONCLUSIONS: The current results are demonstrating a time-dependent correlation between microvessel density and ΔR 2*. Thus, MRI-based evaluation of angiogenesis in the tendon might be a new promising technique for in vivo monitoring of angiogenesis and therapy response in the future.
PURPOSE: Recent in vivo studies were able to show the impairing effect of neoangiogenesis in degenerative tendon diseases. Clinical in vivo monitoring of angiogenesis in injured tendons therefore seems to be crucial for an accurate therapeutic approach. The aim of this study was to develop a novel magnetic resonance imaging (MRI)-based technique for observing angiogenesis during tendon healing in vivo. METHODS:Tendinopathy was induced by an in situ freezing model of ratpatellar tendon and monitored after 7, 14, and 28 days. Animals were randomly divided into an imaging and immunohistochemical group. MRI with a 'blood pool' contrast agent was used to determine neoangiogenesis during tendon healing. MRI was compared to histochemical staining and quantification of blood vessels in injured and native tendons. RESULTS: MRI data revealed a peak in changes in the transverse relaxation rate (ΔR 2*), which is proportional to relative blood volume, 7 days after surgery and decrease until day 28. Histological microvessel density and vascular endothelial growth factor synthesis were also most evident at day 7 and decreased over time. CONCLUSIONS: The current results are demonstrating a time-dependent correlation between microvessel density and ΔR 2*. Thus, MRI-based evaluation of angiogenesis in the tendon might be a new promising technique for in vivo monitoring of angiogenesis and therapy response in the future.
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