Literature DB >> 24510682

Quantitative analysis of copy number variants based on real-time LightCycler PCR.

Lijiang Ma1, Wendy K Chung.   

Abstract

Quantitative real-time PCR is PCR visualized in real time by the use of fluorescent or intercalating dyes, which are employed to measure gene expression or gene quantification including contiguous gene deletions or duplications. A simple method is described here to quantify DNA copy number from human samples.
Copyright © 2014 John Wiley & Sons, Inc.

Entities:  

Keywords:  CNV; copy number analysis; qPCR

Mesh：

Substances：

Year:  2014        PMID： 24510682      PMCID： PMC3949243          DOI： 10.1002/0471142905.hg0721s80

Source DB:  PubMed          Journal:  Curr Protoc Hum Genet        ISSN： 1934-8258


  19 in total

1.  Analyzing real-time PCR data by the comparative C(T) method.

Authors:  Thomas D Schmittgen; Kenneth J Livak
Journal:  Nat Protoc       Date:  2008       Impact factor: 13.491

2.  Quantification of CFTR transcripts.

Authors:  Anabela S Ramalho; Luka A Clarke; Margarida D Amaral
Journal:  Methods Mol Biol       Date:  2011

3.  Real time quantitative PCR.

Authors:  C A Heid; J Stevens; K J Livak; P M Williams
Journal:  Genome Res       Date:  1996-10       Impact factor: 9.043

4.  Detection of HER-2/neu gene amplification in breast carcinomas using quantitative real-time PCR - a comparison with immunohistochemical and FISH results.

Authors:  Janina Kulka; Anna-Mária Tôkés; Pál Kaposi-Novák; Nóra Udvarhelyi; Anikó Keller; Zsuzsa Schaff
Journal:  Pathol Oncol Res       Date:  2006-12-25       Impact factor: 3.201

5.  Evaluation of the efficacy of real-time polymerase chain reaction for the routine early detection of Pseudomonas aeruginosa in cystic fibrosis sputum and throat swab specimens.

Authors:  Catriona Logan; Adele Habington; Gráinne Lennon; Frank Cronin; Niamh O'Sullivan
Journal:  Diagn Microbiol Infect Dis       Date:  2010-09-29       Impact factor: 2.803

6.  Human defensin gene copy number polymorphisms: comprehensive analysis of independent variation in alpha- and beta-defensin regions at 8p22-p23.

Authors:  Rose M Linzmeier; Tomas Ganz
Journal:  Genomics       Date:  2005-10       Impact factor: 5.736

7.  Probe-based quantitative PCR assay for detecting constitutional and somatic deletions in the NF1 gene: application to genetic testing and tumor analysis.

Authors:  Ernest Terribas; Carles Garcia-Linares; Conxi Lázaro; Eduard Serra
Journal:  Clin Chem       Date:  2013-02-05       Impact factor: 8.327

8.  Large deletions of the MECP2 gene detected by gene dosage analysis in patients with Rett syndrome.

Authors:  Franco Laccone; Ivonne Jünemann; Sharon Whatley; Rhian Morgan; Rachel Butler; Peter Huppke; David Ravine
Journal:  Hum Mutat       Date:  2004-03       Impact factor: 4.878

9.  Kinetic PCR analysis: real-time monitoring of DNA amplification reactions.

Authors:  R Higuchi; C Fockler; G Dollinger; R Watson
Journal:  Biotechnology (N Y)       Date:  1993-09

10.  ITS1 copy number varies among Batrachochytrium dendrobatidis strains: implications for qPCR estimates of infection intensity from field-collected amphibian skin swabs.

Authors:  Ana V Longo; David Rodriguez; Domingos da Silva Leite; Luís Felipe Toledo; Cinthya Mendoza Almeralla; Patricia A Burrowes; Kelly R Zamudio
Journal:  PLoS One       Date:  2013-03-21       Impact factor: 3.240
View more
  19 in total

1.  Copy number variation at the HvCBF4-HvCBF2 genomic segment is a major component of frost resistance in barley.

Authors:  Enrico Francia; Caterina Morcia; Marianna Pasquariello; Valentina Mazzamurro; Justyna Anna Milc; Fulvia Rizza; Valeria Terzi; Nicola Pecchioni
Journal:  Plant Mol Biol       Date:  2016-06-23       Impact factor: 4.076

2.  Modulation of N-Methyl-N-nitrosourea Mutagenesis in Mouse Embryo Fibroblasts Derived from the gpt Delta Mouse by an Inhibitor of the O6-Methylguanine Methyltransferase, MGMT.

Authors:  Pennapa Thongararm; Bogdan I Fedeles; Sakunchai Khumsubdee; Amanda L Armijo; Lina Kim; Apinya Thiantanawat; Jeerawan Promvijit; Panida Navasumrit; Mathuros Ruchirawat; Robert G Croy; John M Essigmann
Journal:  Chem Res Toxicol       Date:  2019-12-24       Impact factor: 3.739

3.  Molecular and structural basis of nucleoside diphosphate kinase-mediated regulation of spore and sclerotia development in the fungus Aspergillus flavus.

Authors:  Yu Wang; Sen Wang; Xinyi Nie; Kunlong Yang; Peng Xu; Xiuna Wang; Mengxin Liu; Yongshuai Yang; Zhuo Chen; Shihua Wang
Journal:  J Biol Chem       Date:  2019-06-26       Impact factor: 5.157

4.  C1qa deficiency in mice increases susceptibility to mouse hepatitis virus A59 infection.

Authors:  Han Woong Kim; Sun Min Seo; Jun Young Kim; Jae Hoon Lee; Han Woong Lee; Yang Kyu Choi
Journal:  J Vet Sci       Date:  2021-05       Impact factor: 1.672

Review 5.  A survey of tools for the analysis of quantitative PCR (qPCR) data.

Authors:  Stephan Pabinger; Stefan Rödiger; Albert Kriegner; Klemens Vierlinger; Andreas Weinhäusel
Journal:  Biomol Detect Quantif       Date:  2014-09-06

6.  Overexpression of a Domain of Unknown Function 231-containing protein increases O-xylan acetylation and cellulose biosynthesis in Populus.

Authors:  Yongil Yang; Chang Geun Yoo; Kimberly A Winkeler; Cassandra M Collins; Maud A W Hinchee; Sara S Jawdy; Lee E Gunter; Nancy L Engle; Yunqiao Pu; Xiaohan Yang; Timothy J Tschaplinski; Arthur J Ragauskas; Gerald A Tuskan; Jin-Gui Chen
Journal:  Biotechnol Biofuels       Date:  2017-12-27       Impact factor: 6.040

7.  Engineering Point Mutant and Epitope-Tagged Alleles in Mice Using Cas9 RNA-Guided Nuclease.

Authors:  Marina Gertsenstein; Lauryl M J Nutter
Journal:  Curr Protoc Mouse Biol       Date:  2018-03

8.  Silencing of hepatic fate-conversion factors induce tumorigenesis in reprogrammed hepatic progenitor-like cells.

Authors:  Felipe Serrano; Maria García-Bravo; Marina Blazquez; Josema Torres; Jose V Castell; Jose C Segovia; Roque Bort
Journal:  Stem Cell Res Ther       Date:  2016-07-27       Impact factor: 6.832

9.  A polymorphic (GA/CT)n- SSR influences promoter activity of Tryptophan decarboxylase gene in Catharanthus roseus L. Don.

Authors:  Santosh Kumar; Sabhyata Bhatia
Journal:  Sci Rep       Date:  2016-09-13       Impact factor: 4.379

10.  Integrated Oncogenomic Profiling of Copy Numbers and Gene Expression in Lung Adenocarcinomas without EGFR Mutations or ALK Fusion.

Authors:  Yanzhuo Luo; Bingjin Li; Guangxin Zhang; Yuxiao He; Jeeyoo Hope Bae; Fengping Hu; Ranji Cui; Runhua Liu; Zhou Wang; Lizhong Wang
Journal:  J Cancer       Date:  2018-02-28       Impact factor: 4.207
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.