| Literature DB >> 2451023 |
T Wagenknecht1, R Grassucci, J Frank.
Abstract
Electron micrographs of frozen-hydrated, large ribosomal subunits from Escherichia coli have been analyzed by computer image processing. Images of subunits in the so-called "crown" orientation were analyzed by correlation alignment procedures developed for negatively stained specimens. Averages of the aligned images showed both similarities and differences to averages determined for negatively stained specimens. The L1 ridge is more dense and stalk-like in frozen-hydrated as compared with negatively stained subunits, possibly because it is associated with ribosomal RNA. The results show that it should be feasible to determine the three-dimensional structure of the large ribosomal subunit from micrographs of individual, frozen-hydrated subunits that have been tilted in the electron microscope.Entities:
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Year: 1988 PMID： 2451023 DOI： 10.1016/0022-2836(88)90384-1
Source DB: PubMed Journal: J Mol Biol ISSN： 0022-2836 Impact factor: 5.469