Nader Bagheri1, Fatemeh Azadegan-Dehkordi2, Hosein Sanei3, Afshin Taghikhani4, Ghorbanali Rahimian4, Loghman Salimzadeh2, Morteza Hashemzadeh-Chaleshtori2, Mahmoud Rafieian-kopaei5, Maryam Shirzad5, Hedayatollah Shirzad6. 1. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. 2. Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran. 3. Department of Pathology, Isfahan University of Medical Sciences, Isfahan, Iran. 4. Department of Internal Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran. 5. Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran. 6. Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran. Electronic address: shirzad1951@yahoo.com.
Abstract
OBJECTIVE: Helicobacter pylori (H. pylori) is associated with gastric ulcer and gastric adenocarcinoma. Polymorphisms in the host genes coding for toll-like receptors (TLRs) may influence the innate and adaptive immune response to the infection, affecting the susceptibility to H. pylori or the disease outcomes. But the details and association to different polymorphisms and different clinical expressions in patients infected with H. pylori (different clinical expression of H. pylori infection) remain unclear. METHODS: A case-control study consisting of 195 patients with H. pylori infection and 241 H. pylori uninfection was conducted. Genomic DNA was extracted and genotypes of TLR4Asp299Gly polymorphism were assessed through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Presence of cagA was evaluated using PCR. RESULTS: TLR4 (Asp299Gly) G and DG alleles frequency in H. pylori infected population was significantly higher in the chronic gastritis group than in the chronic active gastritis group (P=0.021; OR, 2.409; 95% CI, 1.124-5.162). Grade mononuclear (MN) infiltration in H. pylori infected patients with DG genotype of TLR-4 Asp299Gly increased significantly. CagA positivity was more frequently associated with chronic active gastritis (P=0.017, OR=2.26, 95% CI=1.144-4.462) and grade polymorphonucler (PMN) infiltration. CONCLUSION: TLR-4 Asp299Gly G allele substitution may be modified pattern of immune response in the gastric mucosa of H. pylori infected patients and may be H. pylori infected patients with gastritis have increased risk for the development of chronic gastritis. CagA positivity may be a risk factor for development of gastritis.
OBJECTIVE:Helicobacter pylori (H. pylori) is associated with gastric ulcer and gastric adenocarcinoma. Polymorphisms in the host genes coding for toll-like receptors (TLRs) may influence the innate and adaptive immune response to the infection, affecting the susceptibility to H. pylori or the disease outcomes. But the details and association to different polymorphisms and different clinical expressions in patientsinfected with H. pylori (different clinical expression of H. pylori infection) remain unclear. METHODS: A case-control study consisting of 195 patients with H. pylori infection and 241 H. pylori uninfection was conducted. Genomic DNA was extracted and genotypes of TLR4Asp299Gly polymorphism were assessed through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Presence of cagA was evaluated using PCR. RESULTS: TLR4 (Asp299Gly) G and DG alleles frequency in H. pylori infected population was significantly higher in the chronic gastritis group than in the chronic active gastritis group (P=0.021; OR, 2.409; 95% CI, 1.124-5.162). Grade mononuclear (MN) infiltration in H. pylori infectedpatients with DG genotype of TLR-4 Asp299Gly increased significantly. CagA positivity was more frequently associated with chronic active gastritis (P=0.017, OR=2.26, 95% CI=1.144-4.462) and grade polymorphonucler (PMN) infiltration. CONCLUSION:TLR-4 Asp299Gly G allele substitution may be modified pattern of immune response in the gastric mucosa of H. pylori infectedpatients and may be H. pylori infectedpatients with gastritis have increased risk for the development of chronic gastritis. CagA positivity may be a risk factor for development of gastritis.
Authors: Vesa-Matti Pohjanen; Olli-Pekka Koivurova; Heikki Huhta; Olli Helminen; Johanna M Mäkinen; Jari M Karhukorpi; Tapio Joensuu; Pentti O Koistinen; Jarno M Valtonen; Seppo E Niemelä; Riitta A Karttunen; Tuomo J Karttunen Journal: PLoS One Date: 2015-07-10 Impact factor: 3.240