Gang Wang1, Zhen Shen1, Fangxing Qian2, Yang Li3, Zhenghong Yuan4, Jun Zhang5. 1. Department of Laboratory Medicine, Shanghai Public Health Clinical Center, Shanghai 201508, China. 2. Department of Infectious Disease, Shanghai Changning Center Hospital, Shanghai 200336, China. 3. Department of Infectious Disease, Shanghai Dongfang Hospital, Shanghai 200120, China. 4. Key Laboratory of Medical Molecular Virology, School of Basic Medical Science, Shanghai Medical College of Fudan University, Shanghai 200030, China. 5. Department of Laboratory Medicine, Shanghai Public Health Clinical Center, Shanghai 201508, China. Electronic address: zhangjun@shaphc.org.
Abstract
BACKGROUND: Sapovirus has been accepted as a major cause of acute gastroenteritis worldwide. It can affect all age groups, ranging from young adults to the elderly, while little is known about the epidemiological patterns and genetic characteristics of sapovirus infections in China. OBJECTIVES: To investigate the prevalence of sapovirus infections among adult outpatients suffering from acute gastroenteritis in Shanghai, China. STUDY DESIGN: From April 2011 to March 2013, fecal specimens from 1125 adult outpatients (≥16 years of age) with acute gastroenteritis were collected. Reverse transcription polymerase chain reaction (RT-PCR) was employed for detection of sapovirus, and 5' end of capsid gene were sequenced for genotyping and phylogenetic analysis. RESULTS: The overall occurrence of sapovirus infection in adult outpatients was 3.73% (42 in 1125) through the two-year surveillance period, and sapovirus diarrhea is more common in spring and winter. The highest sapovirus positive rate was observed in adults of ≥56 years old, and statistically significant relationship was observed when compared with other age groups (p<0.05). Only three genotypes were detected, whereas GI.2 was proved to be the predominant strain, occupying 78.57% (33 in 42) of all strains, followed by GIV, GI.1 and GII.3. CONCLUSIONS: Sapovirus was commonly found in adults with acute gastroenteritis in Shanghai, China, while no specific seasonal variation of sapovirus diarrhea could be distinguished. GI.2 strains established themselves in a short time span as the predominant genotype in Shanghai, China.
BACKGROUND: Sapovirus has been accepted as a major cause of acute gastroenteritis worldwide. It can affect all age groups, ranging from young adults to the elderly, while little is known about the epidemiological patterns and genetic characteristics of sapovirus infections in China. OBJECTIVES: To investigate the prevalence of sapovirus infections among adult outpatients suffering from acute gastroenteritis in Shanghai, China. STUDY DESIGN: From April 2011 to March 2013, fecal specimens from 1125 adult outpatients (≥16 years of age) with acute gastroenteritis were collected. Reverse transcription polymerase chain reaction (RT-PCR) was employed for detection of sapovirus, and 5' end of capsid gene were sequenced for genotyping and phylogenetic analysis. RESULTS: The overall occurrence of sapovirus infection in adult outpatients was 3.73% (42 in 1125) through the two-year surveillance period, and sapovirus diarrhea is more common in spring and winter. The highest sapovirus positive rate was observed in adults of ≥56 years old, and statistically significant relationship was observed when compared with other age groups (p<0.05). Only three genotypes were detected, whereas GI.2 was proved to be the predominant strain, occupying 78.57% (33 in 42) of all strains, followed by GIV, GI.1 and GII.3. CONCLUSIONS: Sapovirus was commonly found in adults with acute gastroenteritis in Shanghai, China, while no specific seasonal variation of sapovirus diarrhea could be distinguished. GI.2 strains established themselves in a short time span as the predominant genotype in Shanghai, China.
Authors: Tammy Kathlyn Amaral Reymão; Juliana das Merces Hernandez; Samya Thalita Picanço da Costa; Maísa Silva de Sousa; Darleise de Souza Oliveira; Luciana Damascena da Silva; Renato da Silva Bandeira; Ian Carlos Gomes de Lima; Luana da Silva Soares; Joana Darc Pereira Mascarenhas; Yvone Benchimol Gabbay Journal: Rev Inst Med Trop Sao Paulo Date: 2016-11-03 Impact factor: 1.846
Authors: Freddy Jun Xian Neo; Jimmy Jin Phang Loh; Peijun Ting; Wei Xin Yeo; Christine Qiu Han Gao; Vernon Jian Ming Lee; Boon Huan Tan; Ching Ging Ng Journal: BMC Infect Dis Date: 2017-11-14 Impact factor: 3.090