Lifeng Zhao1, Xiaohong Shi2, Dequan Meng1, Jiane Guo1, Yiping Li1, Lirong Liang1, Xiaofang Guo1, Ran Tao3, Xiaohua Zhang1, Ruihong Gao1, Li Gao1, Jitao Wang4. 1. Department of Microbiology Test, Taiyuan Center for Disease Control and Prevention, NO. 89 Xinjian South Road, Taiyuan, 030012, Shanxi Province, China. 2. Department of Disease Prevention and Public Health, Children's Hospital of Shanxi, Taiyuan, 030001, Shanxi Province, China. 3. Department of Tuberculosis Control and Prevention, Taiyuan Center for Disease Control and Prevention, Taiyuan, 030012, Shanxi Province, China. 4. Department of Microbiology Test, Taiyuan Center for Disease Control and Prevention, NO. 89 Xinjian South Road, Taiyuan, 030012, Shanxi Province, China. wangjitao99@126.com.
Abstract
BACKGROUND: Group A rotavirus (RVA), despite being a leading cause of gastroenteritis in infants and young children, is less studied in Shanxi Province, China. The current study was conducted to determine the prevalence and genetic characterization of RVA in hospitalized children younger than 10 years of age with the diagnosis of acute gastroenteritis in Shanxi Province, China. METHODS: A hospital-based active surveillance of rotavirus gastroenteritis was conducted at Children's Hospital of Shanxi from Jan 1, 2015, through Dec 31, 2019. Rotavirus was detected in stool samples by real-time quantitative reverse transcription PCR (qRT-PCR). G- and P-genotypes were determined by reverse transcription PCR (RT-PCR) and nucleotide sequencing. RESULTS: A total of 961 children younger than 10 years of age was enrolled over the study period, of whom 183 (19.0%) were positive for RVA. The highest RVA-infection frequency (23.7%) was found among children aged 12-23 months, and the seasonal peak was in December. G9P[8] was most prevalent (76.0%), followed by G3P[8] (7.1%), G2P[4] (3.3%), G1P[8] (0.5%) and G9P[4] (0.5%). CONCLUSIONS: These results report for the first time that RVA was one of the main causes of severe infectious gastroenteritis in children, and a high proportion of G9P[8] strains circulating in most areas of Shanxi Province. While the protective efficacy of the rotavirus vaccines has been demonstrated against G9P[8] strains, our results highlight that the dominant strains have not been effectively controlled in China.
BACKGROUND:Group A rotavirus (RVA), despite being a leading cause of gastroenteritis in infants and young children, is less studied in Shanxi Province, China. The current study was conducted to determine the prevalence and genetic characterization of RVA in hospitalized children younger than 10 years of age with the diagnosis of acute gastroenteritis in Shanxi Province, China. METHODS: A hospital-based active surveillance of rotavirus gastroenteritis was conducted at Children's Hospital of Shanxi from Jan 1, 2015, through Dec 31, 2019. Rotavirus was detected in stool samples by real-time quantitative reverse transcription PCR (qRT-PCR). G- and P-genotypes were determined by reverse transcription PCR (RT-PCR) and nucleotide sequencing. RESULTS: A total of 961 children younger than 10 years of age was enrolled over the study period, of whom 183 (19.0%) were positive for RVA. The highest RVA-infection frequency (23.7%) was found among children aged 12-23 months, and the seasonal peak was in December. G9P[8] was most prevalent (76.0%), followed by G3P[8] (7.1%), G2P[4] (3.3%), G1P[8] (0.5%) and G9P[4] (0.5%). CONCLUSIONS: These results report for the first time that RVA was one of the main causes of severe infectious gastroenteritis in children, and a high proportion of G9P[8] strains circulating in most areas of Shanxi Province. While the protective efficacy of the rotavirus vaccines has been demonstrated against G9P[8] strains, our results highlight that the dominant strains have not been effectively controlled in China.
Entities:
Keywords:
Active surveillance; Acute gastroenteritis; Genotypes; Group a rotavirus
Authors: Sue E Crawford; Sasirekha Ramani; Jacqueline E Tate; Umesh D Parashar; Lennart Svensson; Marie Hagbom; Manuel A Franco; Harry B Greenberg; Miguel O'Ryan; Gagandeep Kang; Ulrich Desselberger; Mary K Estes Journal: Nat Rev Dis Primers Date: 2017-11-09 Impact factor: 52.329
Authors: Sidhartha Giri; Nayana P Nair; Ann Mathew; B Manohar; Anna Simon; Tejinder Singh; S Suresh Kumar; M A Mathew; Sudhir Babji; Rashmi Arora; C P Girish Kumar; S Venkatasubramanian; Sanjay Mehendale; Mohan D Gupte; Gagandeep Kang Journal: BMC Public Health Date: 2019-01-15 Impact factor: 3.295