Effect of gravity and centrifugal acceleration on auxin transport in corn coleoptiles.

Inversion of corn coleoptile sections resulted in a 10-20% inhibition of basipetal transport of 3-indoleacetic acid (IAA) and a more pronounced inhibition (20-50%) of the transport of 1-naphthaleneacetic acid (NAA).The effect of inversion on basipetal NAA transport was compared in wild-type corn and in the amylomaize mutant which contains smaller and slower sedimenting amyloplasts: the gravity induced inhibition was higher in the wild type coleoptiles (27% versus 9%).In wild type the inhibitory effect on basipetal NAA transport appeared within less than 30 min after inversion; then the effect remained relatively constant over at least 2 hr of transport. When the sections were returned to the upright position the transport rate increased, reaching the level of upright controls within 30 min.An effect of gravity on lateral transport of NAA was also demonstrated and shown to be expressed within 10 min after placing the tissue horizontally.When basipetal transport was tested in the direction of gravity and/or centrifugal acceleration, auxin movement incrased with increasing acceleration. Transport against centrifugal acceleration (10 x g) was less than transport of control sections (inverted at 1 x g).The results agree with the hypothesis that starch statoliths act by a pressure mechanism on the membrane transport system of auxin.