Literature DB >> 2450084

Selective staining of fungal hyphae in parasitic and symbiotic plant-fungus associations.

M Meyberg1.   

Abstract

A cytochemical method for light microscopical studies is described which allows the specific detection of fungal hyphae in plant-fungus associations: e.g. lichens, mycorrhiza, or fungal infections of plant tissue. The specimens were fixed and embedded in epoxy resin by a standard protocol for electron microscopy. Semithin sections were successively incubated with fluorescein isothiocyanate labelled wheat germ agglutinin (FITC-WGA) and calcofluor white (CW). FITC-WGA stained exclusively the fungal cell walls while CW stained both the fungal and the plant cell walls. Therefore, FITC-WGA is an excellent marker for the fungal hyphae.

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Year:  1988        PMID： 2450084     DOI： 10.1007/bf00493305

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  16 in total

1.  Interaction of wheat germ agglutinin with sialic acid.

Authors:  B P Peters; S Ebisu; I J Goldstein; M Flashner
Journal:  Biochemistry       Date:  1979-11-27       Impact factor: 3.162

2.  Technical parameters of immunostaining of osmicated tissue in epoxy sections.

Authors:  S L Erlandsen; J A Parsons; C B Rodning
Journal:  J Histochem Cytochem       Date:  1979-09       Impact factor: 2.479

3.  The purification, composition and specificity of wheat-germ agglutinin.

Authors:  A K Allen; A Neuberger; N Sharon
Journal:  Biochem J       Date:  1973-01       Impact factor: 3.857

4.  A low-viscosity epoxy resin embedding medium for electron microscopy.

Authors:  A R Spurr
Journal:  J Ultrastruct Res       Date:  1969-01

5.  Specificity of binding of hexopyranosyl polysaccharides with fluorescent brightener.

Authors:  H Maeda; N Ishida
Journal:  J Biochem       Date:  1967-08       Impact factor: 3.387

6.  Ultrastructural localization of glycoconjugates in the fungus Ascocalyx abietina, the Scleroderris canker agent of conifers, using lectin-gold complexes.

Authors:  N Benhamou; G B Ouellette
Journal:  J Histochem Cytochem       Date:  1986-07       Impact factor: 2.479

7.  Hyphal walls of isolated lichen fungi: autoradiographic localization of precursor incorporation and binding of fluorescein-conjugated lectins.

Authors:  M Galun; A Braun; A Frensdorff; E Galun
Journal:  Arch Microbiol       Date:  1976-05-03       Impact factor: 2.552

8.  Immunocytochemical demonstration of LH-RH, somatostatin, and ACTH-like peptide in osmium-postfixed, resin-embedded median eminence.

Authors:  J C Beauvillain; G Tramu
Journal:  J Histochem Cytochem       Date:  1980-09       Impact factor: 2.479

9.  Localization of chitin in the cell wall of Candida albicans by means of wheat germ agglutinin. Fluorescence and ultrastructural studies.

Authors:  G Tronchin; D Poulain; J Herbaut; J Biguet
Journal:  Eur J Cell Biol       Date:  1981-12       Impact factor: 4.492

10.  Studies on growth inhibition by lectins of Penicillia and Aspergilli.

Authors:  R Barkai-Golan; D Mirelman; N Sharon
Journal:  Arch Microbiol       Date:  1978-02       Impact factor: 2.552
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  7 in total

1.  Secreted fungal effector lipase releases free fatty acids to inhibit innate immunity-related callose formation during wheat head infection.

Authors:  Antje Blümke; Christian Falter; Cornelia Herrfurth; Björn Sode; Rainer Bode; Wilhelm Schäfer; Ivo Feussner; Christian A Voigt
Journal:  Plant Physiol       Date:  2014-03-31       Impact factor: 8.340

2.  Intercomparison of Two Fluorescent Dyes to Visualize Parasitic Fungi (Chytridiomycota) on Phytoplankton.

Authors:  Isabell Klawonn; Susanne Dunker; Maiko Kagami; Hans-Peter Grossart; Silke Van den Wyngaert
Journal:  Microb Ecol       Date:  2021-12-02       Impact factor: 4.192

3.  Semiautomated confocal imaging of fungal pathogenesis on plants: Microscopic analysis of macroscopic specimens.

Authors:  Katharine R Minker; Meredith L Biedrzycki; Abhishek Kolagunda; Stephen Rhein; Fabiano J Perina; Samuel S Jacobs; Michael Moore; Tiffany M Jamann; Qin Yang; Rebecca Nelson; Peter Balint-Kurti; Chandra Kambhamettu; Randall J Wisser; Jeffrey L Caplan
Journal:  Microsc Res Tech       Date:  2016-06-25       Impact factor: 2.769

4.  Multiplex real-time PCR for monitoring Heterobasidion annosum colonization in Norway spruce clones that differ in disease resistance.

Authors:  Ari M Hietala; Morten Eikenes; Harald Kvaalen; Halvor Solheim; Carl G Fossdal
Journal:  Appl Environ Microbiol       Date:  2003-08       Impact factor: 4.792

5.  Three-dimensional ultrastructural study of the anther of Silene latifolia infected with Microbotryum lychnidis-dioicae.

Authors:  Hiroki Kawamoto; Aiko Hirata; Shigeyuki Kawano
Journal:  PLoS One       Date:  2017-08-09       Impact factor: 3.240

6.  Characterizing the "fungal shunt": Parasitic fungi on diatoms affect carbon flow and bacterial communities in aquatic microbial food webs.

Authors:  Isabell Klawonn; Silke Van den Wyngaert; Alma E Parada; Nestor Arandia-Gorostidi; Martin J Whitehouse; Hans-Peter Grossart; Anne E Dekas
Journal:  Proc Natl Acad Sci U S A       Date:  2021-06-08       Impact factor: 11.205

7.  Micro-scale Experimental System Coupled with Fluorescence-based Estimation of Fungal Biomass to Study Utilisation of Plant Substrates.

Authors:  Julianna B Németh; Dániel G Knapp; Annamária Kósa; Panna Á Hegedűs; Gábor Herczeg; Pál Vági; Gábor M Kovács
Journal:  Microb Ecol       Date:  2021-07-03       Impact factor: 4.552
  7 in total

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