Rong Ma1, Baihan Gu, Yang Gu, Lynn J Groome, Yuping Wang. 1. Departments of Obstetrics and Gynecology, LSU Health Sciences Center, Shreveport, LA, USA; Department of Gynecology, The Third Hospital, Harbin Medical University, Harbin, China.
Abstract
PROBLEM: To determine whether down-regulation of TIMP3 expression promotes TACE expression and increases in TNFα production by placental trophoblast cells. METHOD OF STUDY: Placental expression of TIMP3 and TACE was examined by immunostaining and Western blot. Effects of TIMP3 on TACE expression and TNFα production were assessed by transfection of TIMP3 siRNA into trophoblasts isolated from normal placentas. Effects of oxidative stress on trophoblast TIMP3 expression and TNFα production were also determined. Trophoblast production of TIMP3, TACE and TNFα were measured by ELISA. RESULTS: TIMP3 expression was markedly reduced in preeclamptic placentas compared with normal placentas; oxidative stress down-regulated trophoblast TIMP3 expression and production, P < 0.01. Down-regulation of TIMP3 expression by TIMP3 siRNA resulted in significant increases in TACE expression and TNFα production, P < 0.01. CONCLUSION: As TIMP3 is an endogenous TACE inhibitor, down-regulation of trophoblast TIMP3 expression/activity could result in increased TACE expression and subsequently lead to increased TNFα production in preeclamptic placentas.
PROBLEM: To determine whether down-regulation of TIMP3 expression promotes TACE expression and increases in TNFα production by placental trophoblast cells. METHOD OF STUDY: Placental expression of TIMP3 and TACE was examined by immunostaining and Western blot. Effects of TIMP3 on TACE expression and TNFα production were assessed by transfection of TIMP3 siRNA into trophoblasts isolated from normal placentas. Effects of oxidative stress on trophoblast TIMP3 expression and TNFα production were also determined. Trophoblast production of TIMP3, TACE and TNFα were measured by ELISA. RESULTS:TIMP3 expression was markedly reduced in preeclamptic placentas compared with normal placentas; oxidative stress down-regulated trophoblast TIMP3 expression and production, P < 0.01. Down-regulation of TIMP3 expression by TIMP3 siRNA resulted in significant increases in TACE expression and TNFα production, P < 0.01. CONCLUSION: As TIMP3 is an endogenous TACE inhibitor, down-regulation of trophoblast TIMP3 expression/activity could result in increased TACE expression and subsequently lead to increased TNFα production in preeclamptic placentas.
Authors: A Amour; C G Knight; A Webster; P M Slocombe; P E Stephens; V Knäuper; A J Docherty; G Murphy Journal: FEBS Lett Date: 2000-05-19 Impact factor: 4.124
Authors: Ratnadeep Basu; Jiwon Lee; Zuocheng Wang; Vaibhav B Patel; Dong Fan; Subhash K Das; George C Liu; Rohan John; James W Scholey; Gavin Y Oudit; Zamaneh Kassiri Journal: Am J Physiol Renal Physiol Date: 2012-08-15
Authors: Fazilat F Mohammed; David S Smookler; Suzanne E M Taylor; Barbara Fingleton; Zamaneh Kassiri; Otto H Sanchez; Jane L English; Lynn M Matrisian; Billie Au; Wen-Chen Yeh; Rama Khokha Journal: Nat Genet Date: 2004-08-22 Impact factor: 38.330
Authors: Sandra Bauer; Jürgen Pollheimer; Johannes Hartmann; Peter Husslein; John D Aplin; Martin Knöfler Journal: J Clin Endocrinol Metab Date: 2004-02 Impact factor: 5.958
Authors: R Pijnenborg; P J McLaughlin; L Vercruysse; M Hanssens; P M Johnson; J C Keith; F A Van Assche Journal: Placenta Date: 1998-05 Impact factor: 3.481
Authors: Griselda A Cabral-Pacheco; Idalia Garza-Veloz; Claudia Castruita-De la Rosa; Jesús M Ramirez-Acuña; Braulio A Perez-Romero; Jesús F Guerrero-Rodriguez; Nadia Martinez-Avila; Margarita L Martinez-Fierro Journal: Int J Mol Sci Date: 2020-12-20 Impact factor: 5.923
Authors: Don L Armstrong; Michael R McGowen; Amy Weckle; Priyadarshini Pantham; Jason Caravas; Dalen Agnew; Kurt Benirschke; Sue Savage-Rumbaugh; Eviatar Nevo; Chong J Kim; Günter P Wagner; Roberto Romero; Derek E Wildman Journal: Placenta Date: 2017-05-12 Impact factor: 3.481