Literature DB >> 2449164

Direct measurement of inositol in bovine myelin basic protein.

R Smith1, P E Braun, M A Ferguson, M G Low, W R Sherman.   

Abstract

Myelin basic protein has been isolated from bovine central-nervous-system myelin by four methods, none of which exposes the protein to acid. After purification the inositol content of both hydrolysed and unhydrolysed protein was quantified by g.c.-m.s. Basic protein prepared by all methods contained less than 4 mol % of inositol. It is concluded, contrary to a previous proposal, that covalent binding to phosphoinositides does not represent a general mechanism for attachment of this cytoplasmically-oriented protein to its membrane.

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Year:  1987        PMID: 2449164      PMCID: PMC1148533          DOI: 10.1042/bj2480285

Source DB:  PubMed          Journal:  Biochem J        ISSN: 0264-6021            Impact factor:   3.857


  13 in total

1.  Purification and comparison of 2',3'-cyclic nucleotide 3'-phosphohydrolases from bovine brain and spinal cord.

Authors:  R J Drummond; E B Hamill; A Guha
Journal:  J Neurochem       Date:  1978-10       Impact factor: 5.372

2.  Covalent linkage of phospholipid to myelin basic protein: identification of serine-54 as the site of attachment.

Authors:  P C Chang; J C Yang; J M Fujitaki; K C Chiu; R A Smith
Journal:  Biochemistry       Date:  1986-05-06       Impact factor: 3.162

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Removal of covalently bound inositol from Torpedo acetylcholinesterase and mammalian alkaline phosphatases by deamination with nitrous acid. Evidence for a common membrane-anchoring structure.

Authors:  M G Low; A H Futerman; K E Ackermann; W R Sherman; I Silman
Journal:  Biochem J       Date:  1987-01-15       Impact factor: 3.857

5.  Measurement of myo-inositol in single cells and defined areas of the nervous system by selected ion monitoring.

Authors:  W R Sherman; P M Packman; M H Laird; R L Boshans
Journal:  Anal Biochem       Date:  1977-03       Impact factor: 3.365

6.  Identification of covalently bound inositol in the hydrophobic membrane-anchoring domain of Torpedo acetylcholinesterase.

Authors:  A H Futerman; M G Low; K E Ackermann; W R Sherman; I Silman
Journal:  Biochem Biophys Res Commun       Date:  1985-05-31       Impact factor: 3.575

7.  Non-protein constituents in myelin basic protein preparations.

Authors:  E P Lapin; H S Maker; H I Schipper; C Weiss; G M Lehrere
Journal:  J Neurochem       Date:  1976-06       Impact factor: 5.372

8.  Glycosyl-sn-1,2-dimyristylphosphatidylinositol is covalently linked to Trypanosoma brucei variant surface glycoprotein.

Authors:  M A Ferguson; M G Low; G A Cross
Journal:  J Biol Chem       Date:  1985-11-25       Impact factor: 5.157

9.  Fatty acid acylation of rat brain myelin proteolipid protein in vitro: identification of the lipid donor.

Authors:  O A Bizzozero; M B Lees
Journal:  J Neurochem       Date:  1986-02       Impact factor: 5.372

10.  In vivo acylation of proteolipid protein and DM-20 in myelin and myelin subfractions of developing rat brain: immunoblot identification of acylated PLP and DM-20.

Authors:  M M Garwood; W R Gilbert; H C Agrawal
Journal:  Neurochem Res       Date:  1983-05       Impact factor: 3.996

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  8 in total

1.  Isolation and characterization of glycosylphosphatidylinositol-anchored, mucin-like surface glycoproteins from bloodstream forms of the freshwater-fish parasite Trypanosoma carassii.

Authors:  A Lischke; C Klein; Y D Stierhof; M Hempel; A Mehlert; I C Almeida; M A Ferguson; P Overath
Journal:  Biochem J       Date:  2000-02-01       Impact factor: 3.857

2.  Myelin basic protein does not contain a phosphatidylinositol anchor.

Authors:  J McLaurin; C Hew; M A Moscarello
Journal:  Biochem J       Date:  1990-07-01       Impact factor: 3.857

3.  In vivo labeling of myelin lipids and proteolipid protein with [3H]myristate, [14C]linoleate, and [14C]linolenate.

Authors:  P Bürgisser; J M Matthieu
Journal:  Neurochem Res       Date:  1989-01       Impact factor: 3.996

4.  Ectoenzymes of the kidney microvillar membrane. Affinity purification, characterization and localization of the phospholipase C-solubilized form of renal dipeptidase.

Authors:  G M Littlewood; N M Hooper; A J Turner
Journal:  Biochem J       Date:  1989-01-15       Impact factor: 3.857

5.  Structure of the CAMPATH-1 antigen, a glycosylphosphatidylinositol-anchored glycoprotein which is an exceptionally good target for complement lysis.

Authors:  M Q Xia; G Hale; M R Lifely; M A Ferguson; D Campbell; L Packman; H Waldmann
Journal:  Biochem J       Date:  1993-08-01       Impact factor: 3.857

6.  Lytic anti-alpha-galactosyl antibodies from patients with chronic Chagas' disease recognize novel O-linked oligosaccharides on mucin-like glycosyl-phosphatidylinositol-anchored glycoproteins of Trypanosoma cruzi.

Authors:  I C Almeida; M A Ferguson; S Schenkman; L R Travassos
Journal:  Biochem J       Date:  1994-12-15       Impact factor: 3.857

7.  Characterization of glycoinositol phospholipids in the amastigote stage of the protozoan parasite Leishmania major.

Authors:  P Schneider; J P Rosat; A Ransijn; M A Ferguson; M J McConville
Journal:  Biochem J       Date:  1993-10-15       Impact factor: 3.857

8.  The initial events in myelin synthesis: orientation of proteolipid protein in the plasma membrane of cultured oligodendrocytes.

Authors:  L D Hudson; V L Friedrich; T Behar; M Dubois-Dalcq; R A Lazzarini
Journal:  J Cell Biol       Date:  1989-08       Impact factor: 10.539

  8 in total

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