| Literature DB >> 2449164 |
R Smith1, P E Braun, M A Ferguson, M G Low, W R Sherman.
Abstract
Myelin basic protein has been isolated from bovine central-nervous-system myelin by four methods, none of which exposes the protein to acid. After purification the inositol content of both hydrolysed and unhydrolysed protein was quantified by g.c.-m.s. Basic protein prepared by all methods contained less than 4 mol % of inositol. It is concluded, contrary to a previous proposal, that covalent binding to phosphoinositides does not represent a general mechanism for attachment of this cytoplasmically-oriented protein to its membrane.Entities:
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Year: 1987 PMID: 2449164 PMCID: PMC1148533 DOI: 10.1042/bj2480285
Source DB: PubMed Journal: Biochem J ISSN: 0264-6021 Impact factor: 3.857