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Literature DB >> 2448310

Nuclear transport in 3T3 fibroblasts: effects of growth factors, transformation, and cell shape.

Abstract

Nucleocytoplasmic transport of fluorescent-labeled macromolecules was investigated in transformed and nontransformed 3T3 fibroblasts. Insulin and epidermal growth factor enhanced transport three-fold after 1-2-h incubation with nontransformed adhering fibroblasts; no enhancement of transport was observed for spherical unattached fibroblasts. The concentration of growth factor for maximal enhancement was 3-10 nM. Nuclear transport for Kirsten murine sarcoma virus-transformed BALB/c 3T3 fibroblasts, however, was maximally enhanced before addition of growth factors; addition of insulin or epidermal growth factor causes no additional transport enhancement. Transformation also minimizes cell shape effects on macromolecular nuclear transport. These results provide evidence that protein growth factors and oncogenic transformation may use a similar mechanism for activation of nuclear transport.

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Year: 1988 PMID： 2448310 PMCID： PMC2114954 DOI： 10.1083/jcb.106.1.13

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

35 in total

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Journal: Proc Natl Acad Sci U S A Date: 1983-03 Impact factor: 11.205

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Journal: Biochemistry Date: 1979-08-21 Impact factor: 3.162

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Authors: I Lang; M Scholz; R Peters
Journal: J Cell Biol Date: 1986-04 Impact factor: 10.539

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Authors: M Schindler; L W Jiang
Journal: J Cell Biol Date: 1987-04 Impact factor: 10.539

14 in total

1. Conformational changes of the in situ nuclear pore complex.

Authors: H Wang; D E Clapham
Journal: Biophys J Date: 1999-07 Impact factor: 4.033

2. Nuclear activities of basic fibroblast growth factor: potentiation of low-serum growth mediated by natural or chimeric nuclear localization signals.

Authors: M Arese; Y Chen; R Z Florkiewicz; A Gualandris; B Shen; D B Rifkin
Journal: Mol Biol Cell Date: 1999-05 Impact factor: 4.138

3. The effects of cyclic stretch on gene transfer in alveolar epithelial cells.

Authors: Winna Taylor; Kerimi E Gokay; Chris Capaccio; Erica Davis; Matthew Glucksberg; David A Dean
Journal: Mol Ther Date: 2003-04 Impact factor: 11.454

4. Neurons and glia arise from a common progenitor in chicken optic tectum: demonstration with two retroviruses and cell type-specific antibodies.

Authors: D S Galileo; G E Gray; G C Owens; J Majors; J R Sanes
Journal: Proc Natl Acad Sci U S A Date: 1990-01 Impact factor: 11.205

5. Inflammation Drives Retraction, Stiffening, and Nodule Formation via Cytoskeletal Machinery in a Three-Dimensional Culture Model of Aortic Stenosis.

Authors: Jina Lim; Arshia Ehsanipour; Jeffrey J Hsu; Jinxiu Lu; Taylor Pedego; Alexander Wu; Chris M Walthers; Linda L Demer; Stephanie K Seidlits; Yin Tintut
Journal: Am J Pathol Date: 2016-07-05 Impact factor: 4.307

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Journal: Proc Natl Acad Sci U S A Date: 1990-05 Impact factor: 11.205

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Journal: J Virol Date: 1992-12 Impact factor: 5.103

10. Nucleocytoplasmic transport is enhanced concomitant with nuclear accumulation of epidermal growth factor (EGF) binding activity in both 3T3-1 and EGF receptor reconstituted NR-6 fibroblasts.

Authors: L W Jiang; M Schindler
Journal: J Cell Biol Date: 1990-03 Impact factor: 10.539