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Literature DB >> 24481519

Detection and quantitative determination of abscisic acid by immunological assay.

Abstract

Antibodies with specificity towards abscisic acid (ABA) were produced in rabbits. These antibodies were used for assaying ABA by the inhibition of inactivation of modified bacteriophage. For this assay conjugates of ABA with bacteriophage T4 were prepared and characterized. Such chemically modified bacteriophages were completely inactivated by the specific anti-ABA serum and this inactivation was inhibited by free ABA. The identification and quantitative determination of ABA in plant extracts by this method are demonstrated and the method is compared with a common bioassay.

Entities: Chemical Species

Year: 1972 PMID： 24481519 DOI： 10.1007/BF00387362

Source DB: PubMed Journal: Planta ISSN： 0032-0935 Impact factor: 4.116

14 in total

1. Studies on the chemical basis of the antigenicity of proteins. 6. Antigenic specificity of some synthetic polypeptides containing tyrosine.

Authors: S FUCHS; M SELA
Journal: Biochem J Date: 1963-04 Impact factor: 3.857

2. The reversible reduction of disulfide bonds in polyalanyl ribonuclease.

Authors: C B ANFINSEN; M SELA; J P COOKE
Journal: J Biol Chem Date: 1962-06 Impact factor: 5.157

3. Immunoassay of endogenous plasma insulin in man.

Authors: R S YALOW; S A BERSON
Journal: J Clin Invest Date: 1960-07 Impact factor: 14.808

4. Studies on the Growth of Coleoptile and First Internode Sections. A New, Sensitive, Straight-Growth Test for Auxins.

Authors: J P Nitsch; C Nitsch
Journal: Plant Physiol Date: 1956-03 Impact factor: 8.340

5. Antigenicity of 5-hydroxyindole-3-acetic acid, a derivative of serotonin. I. Preparation of protein conjugates of 5-hydroxyindole-3-acetic acid.

Authors: N S Ranadive; A H Sehon
Journal: Can J Biochem Date: 1967-11

6. Immunological assay for plant hormones using specific antibodies to indoleacetic acid and gibberellic acid.

Authors: S Fuchs; Y Fuchs
Journal: Biochim Biophys Acta Date: 1969-12-30

7. Preparation of protein-bacteriophage conjugates and their use in detection of anti-protein antibodies.

Authors: J Haimovich; E Hurwitz; N Novik; M Sela
Journal: Biochim Biophys Acta Date: 1970-04-28

8. Immunological studies of plant hormones. Detection and estimation by immunological assays.

Authors: S Fuchs; J Haimovich; Y Fuchs
Journal: Eur J Biochem Date: 1971-02-01

9. Combining sites of antibodies with L-alanine and D-alanine peptide specificity and the effect of serum proteolytic activity on their estimation.

Authors: I Schechter; B Schechter; M Sela
Journal: Biochim Biophys Acta Date: 1966-10-31

3. A monoclonal antibody to (S)-abscisic acid: its characterisation and use in a radioimmunoassay for measuring abscisic acid in crude extracts of cereal and lupin leaves.

Authors: S A Quarrie; P N Whitford; N E Appleford; T L Wang; S K Cook; I E Henson; B R Loveys
Journal: Planta Date: 1988-03 Impact factor: 4.116

4. Validation of a radioimmunoassay for (+)-abscisic acid in extracts of apple and sweet-pepper tissue using high-pressure liquid chromatography and combined gas chromatography-mass spectrometry.

Authors: P H Rosher; H G Jones; P Hedden
Journal: Planta Date: 1985-07 Impact factor: 4.116

4 in total