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Literature DB >> 24318051

A radioimmunoassay for abscisic acid.

Abstract

We have developed a radioimmunoassay (RIA) for abscisic acid (ABA) in the 0.1 ng to 2.5 ng range. Antibodies were obtained from rabbits immunized with ABA bound via its carboxyl group to bovine serum albumin. Cross-reactivity studies indicate that ABA esters are completely cross-reactive with ABA, while trans, trans abscisic acid (t-ABA) phaseic acid (PA) and dihydrophaseic acid (DPA) have much lower but significant cross-reactivities. Purification methods which reduce the levels of cross-reacting substances are described.

Entities: Chemical Gene Species

Year: 1979 PMID： 24318051 DOI： 10.1007/BF00388224

Source DB: PubMed Journal: Planta ISSN： 0032-0935 Impact factor: 4.116

17 in total

1. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.

Authors: S W Kessler
Journal: J Immunol Date: 1975-12 Impact factor: 5.422

2. Use of specific antibodies for quantification of steroid hormones.

Authors: G D Niswender; A M Akbar; T M Nett
Journal: Methods Enzymol Date: 1975 Impact factor: 1.600

3. Glycosylation of free trans-trans abscisic acid as a contributing factor in bud dormancy break.

Authors: Y Leshem; S Philosoph; J Wurzburger
Journal: Biochem Biophys Res Commun Date: 1974-03-25 Impact factor: 3.575

4. Electron capture-gas chromatography for sensitive assay of abscisic acid.

Authors: S D Seeley; L E Powell
Journal: Anal Biochem Date: 1970-06 Impact factor: 3.365

5. Detection and quantitative determination of abscisic acid by immunological assay.

Authors: Y Fuchs; S Mayak; S Fuchs
Journal: Planta Date: 1972-06 Impact factor: 4.116

6. Endogenous abscisic Acid in relation to bud growth in alternate bearing ;valencia' orange.

Authors: W W Jones; C W Coggins; T W Embleton
Journal: Plant Physiol Date: 1976-11 Impact factor: 8.340

7. Sites of Abscisic Acid Synthesis and Metabolism in Ricinus communis L.

Authors: J A Zeevaart
Journal: Plant Physiol Date: 1977-05 Impact factor: 8.340

8. The isolation of an abscisic-acid metabolite, 4'-dihydrophaseic acid, from non-imbibed Phaseolus vulgaris seed.

Authors: D C Walton; B Dorn; J Fey
Journal: Planta Date: 1973-03 Impact factor: 4.116

9. Radioimmunoassay for the measurement of adenosine 3',5'-cyclic phosphate.

Authors: A L Steiner; D M Kipnis; R Utiger; C Parker
Journal: Proc Natl Acad Sci U S A Date: 1969-09 Impact factor: 11.205

10. Structure-activity correlations with compounds related to abscisic acid.

Authors: E Sondheimer; D C Walton
Journal: Plant Physiol Date: 1970-03 Impact factor: 8.340

8 in total

Review 1. Quo vadis plant hormone analysis?

Authors: Danuše Tarkowská; Ondřej Novák; Kristýna Floková; Petr Tarkowski; Veronika Turečková; Jiří Grúz; Jakub Rolčík; Miroslav Strnad
Journal: Planta Date: 2014-03-28 Impact factor: 4.116

5. A monoclonal antibody to (S)-abscisic acid: its characterisation and use in a radioimmunoassay for measuring abscisic acid in crude extracts of cereal and lupin leaves.

Authors: S A Quarrie; P N Whitford; N E Appleford; T L Wang; S K Cook; I E Henson; B R Loveys
Journal: Planta Date: 1988-03 Impact factor: 4.116

6. Validation of a radioimmunoassay for (+)-abscisic acid in extracts of apple and sweet-pepper tissue using high-pressure liquid chromatography and combined gas chromatography-mass spectrometry.

Authors: P H Rosher; H G Jones; P Hedden
Journal: Planta Date: 1985-07 Impact factor: 4.116

7. Measurement of endogenous ABA levels in chilled somatic embryos of carrot by immunoassay.

Authors: T M Spencer; S L Kitto
Journal: Plant Cell Rep Date: 1988-08 Impact factor: 4.570

8. Levels of indole-3-acetic acid in intact and decapitated coleoptiles as determined by a specific and highly sensitive solid-phase enzyme immunoassay.

Authors: E W Weiler; P S Jourdan; W Conrad
Journal: Planta Date: 1981-12 Impact factor: 4.116