Ahmet Ekmekçi1, Mahmut Uluganyan2, Barış Güngör3, Neslihan Abacı4, Kazım Serhan Ozcan3, Gökhan Ertaş3, Aycan Zencirci3, Ahmet Yavuz Balcı5, Sema Sırma Ekmekci4, Nurten Sayar3, Duran Ustek4, Mehmet Eren3. 1. Department of Cardiology, Siyami Ersek Cardiovascular and Thoracic Surgery Center, İstanbul, Turkey. ahmetekmekci@yahoo.com. 2. Department of Cardiology, Kadirli State Hospital, Osmaniye, Turkey. 3. Department of Cardiology, Siyami Ersek Cardiovascular and Thoracic Surgery Center, İstanbul, Turkey. 4. Department of Genetics, İstanbul University, Institute for Experimental Medical Research, İstanbul, Turkey. 5. Department of Cardiovascular Surgery, Siyami Ersek Cardiovascular and Thoracic Surgery Center, İstanbul, Turkey.
Abstract
OBJECTIVES: The genetic risk factors that contribute to the risk of developing aortic dissection (AD) have been studied. We assessed the association of endothelial nitric oxide synthase (eNOS) gene polymorphism with AD. STUDY DESIGN: Patients who underwent surgery with the diagnosis of AD and survived after the operation in our center between May 2007 and June 2011 were recruited retrospectively. The eNOS intron 4a/b polymorphism was determined by polymerase chain reaction (PCR) using oligonucleotide primers (sense: 5'-AGGCCCTATGGTAGTGCCTTT-3'; antisense: 5'-TCTCTTAGTGCTGTGGTCAC-3') that flank the region of the 27 bp VNTR in intron 4. RESULTS: Thirty-nine patients (88%) had type A AD, while the remainder (12%) had type B AD. The distribution of eNOS4 a/b gene polymorphism differed significantly from the control group, with higher frequencies of eNOS 4a/a and 4a/b genotypes in the AD group (x(2)=7.16, p=0.03). CONCLUSION: In this study, the distribution of eNOS genotypes differed between the AD and control groups; however, this polymorphism was not found to be an independent factor for the development of AD.
OBJECTIVES: The genetic risk factors that contribute to the risk of developing aortic dissection (AD) have been studied. We assessed the association of endothelial nitric oxide synthase (eNOS) gene polymorphism with AD. STUDY DESIGN:Patients who underwent surgery with the diagnosis of AD and survived after the operation in our center between May 2007 and June 2011 were recruited retrospectively. The eNOS intron 4a/b polymorphism was determined by polymerase chain reaction (PCR) using oligonucleotide primers (sense: 5'-AGGCCCTATGGTAGTGCCTTT-3'; antisense: 5'-TCTCTTAGTGCTGTGGTCAC-3') that flank the region of the 27 bp VNTR in intron 4. RESULTS: Thirty-nine patients (88%) had type A AD, while the remainder (12%) had type B AD. The distribution of eNOS4 a/b gene polymorphism differed significantly from the control group, with higher frequencies of eNOS 4a/a and 4a/b genotypes in the AD group (x(2)=7.16, p=0.03). CONCLUSION: In this study, the distribution of eNOS genotypes differed between the AD and control groups; however, this polymorphism was not found to be an independent factor for the development of AD.
Authors: Ataç Çelik; Mustafa Özçetin; Ömer Ateş; Fatih Altunkaş; Kayıhan Karaman; İlker Akar; İlker İnce; Murat Yalçın; Metin Karayakalı; Köksal Ceyhan; Fatih Koç Journal: Balkan Med J Date: 2015-10-01 Impact factor: 2.021
Authors: Joshua C Peterson; Lambertus J Wisse; Valerie Wirokromo; Tessa van Herwaarden; Anke M Smits; Adriana C Gittenberger-de Groot; Marie-José T H Goumans; J Conny VanMunsteren; Monique R M Jongbloed; Marco C DeRuiter Journal: Dis Model Mech Date: 2020-09-28 Impact factor: 5.758