Mahmoud Rafieian-Kopaei1, Hamid Nasri2, Mehdi Nematbakhsh3, Azar Baradaran4, Alaleh Gheissari5, Hamid Rouhi1, Seyed Mohammad Ahmadi Soleimani6, Milad Baradaran-Ghahfarokhi7, Fatemeh Ghaed-Amini1, Mohammadreza Ardalan8. 1. Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran. 2. Department of Nephrology, Division of Nephropathology, Isfahan University of Medical Sciences, Isfahan, Iran. 3. Water and Electrolytes Research Center, Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran. 4. Department of Clinical Pathology, Isfahan University of Medical Sciences, Isfahan , Iran. 5. Isfahan Kidney Disease Research Center, Isfahan University of Medical Sciences, Isfahan , Iran. 6. Department of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. 7. Medical Physics and Medical Engineering Department, Isfahan University of Medical Sciences, Isfahan, Iran. 8. Department of Nephrology, Tabriz University of Medical Sciences, Tabriz, Iran.
Abstract
BACKGROUND: Investigations have attempted to modify the outcome of tubular injury by either ameliorating renal tubular damage or promoting tubular regeneration in the case of acute tubular necrosis. OBJECTIVES: We investigated the protective effect of Eprex an erythropoietin analogue on tubular injury induced by gentamicin (GM). MATERIALS AND METHODS: Forty male Wistar rats were randomly divided into four groups. In group 1,rats were served as a sham group. In group 2, rats were injected intraperitoneally with 100 mg/kg of GM for 10 consecutive days (positive control group) and then were sacrificed. In group 3, rats received GM for 10 days then Eprex 100U/kg was injected intraperitoneally for the next 10 days and then they were sacrificed at the day 20th. In group 4 rats were injected a combination of GM (80 mg/kg) and Eprex 100U/kg intraperitoneally for 10 days and then were sacrificed. RESULTS: The results indicated that, Eprex prevented the increase in serum creatinine (Cr) and blood urea nitrogen (BUN). The effect of Eprex on damage score, showed that co-administration of GM and Eprex (group 3 and 4) reduced the kidney tissue damage compared to positive control group (P<0.05). This result indicat that Eprex potentially can reduce or prevent the kidney tissue damage. CONCLUSIONS: Ameliorative effect of Eprex when the drug was given in combination with GM and also when the drug was applied after GM-induced tubular damage, revealed the renoprotective potency of Eprex. Eprex is a promising drug to prevent or attenuate tubular damage induced by GM or other nephrotoxic agents which act through the same mechanisms as gentamicin.
BACKGROUND: Investigations have attempted to modify the outcome of tubular injury by either ameliorating renal tubular damage or promoting tubular regeneration in the case of acute tubular necrosis. OBJECTIVES: We investigated the protective effect of Eprex an erythropoietin analogue on tubular injury induced by gentamicin (GM). MATERIALS AND METHODS: Forty male Wistar rats were randomly divided into four groups. In group 1,rats were served as a sham group. In group 2, rats were injected intraperitoneally with 100 mg/kg of GM for 10 consecutive days (positive control group) and then were sacrificed. In group 3, rats received GM for 10 days then Eprex 100U/kg was injected intraperitoneally for the next 10 days and then they were sacrificed at the day 20th. In group 4 rats were injected a combination of GM (80 mg/kg) and Eprex 100U/kg intraperitoneally for 10 days and then were sacrificed. RESULTS: The results indicated that, Eprex prevented the increase in serum creatinine (Cr) and blood ureanitrogen (BUN). The effect of Eprex on damage score, showed that co-administration of GM and Eprex (group 3 and 4) reduced the kidney tissue damage compared to positive control group (P<0.05). This result indicat that Eprex potentially can reduce or prevent the kidney tissue damage. CONCLUSIONS: Ameliorative effect of Eprex when the drug was given in combination with GM and also when the drug was applied after GM-induced tubular damage, revealed the renoprotective potency of Eprex. Eprex is a promising drug to prevent or attenuate tubular damage induced by GM or other nephrotoxic agents which act through the same mechanisms as gentamicin.
Authors: Young Rim Song; Taewoo Lee; Sun Jin You; Ho Joon Chin; Dong-Wan Chae; Cheong Lim; Kay-Hyun Park; Sunghee Han; Jin-Hee Kim; Ki Young Na Journal: Am J Nephrol Date: 2009-06-02 Impact factor: 3.754
Authors: Chul Woo Yang; Can Li; Ju Young Jung; Seok Joon Shin; Bum Soon Choi; Sun Woo Lim; Bo Kyung Sun; Yong Soo Kim; Jin Kim; Yoon Sik Chang; Byung Kee Bang Journal: FASEB J Date: 2003-07-18 Impact factor: 5.191
Authors: Zoltán H Endre; Robert J Walker; John W Pickering; Geoffrey M Shaw; Christopher M Frampton; Seton J Henderson; Robyn Hutchison; Jan E Mehrtens; Jillian M Robinson; John B W Schollum; Justin Westhuyzen; Leo A Celi; Robert J McGinley; Isaac J Campbell; Peter M George Journal: Kidney Int Date: 2010-02-17 Impact factor: 10.612