Dichloromethane removal and microbial variations in a combination of UV pretreatment and biotrickling filtration.

Biofiltration of hydrophobic and/or recalcitrant volatile organic compounds in industry is currently limited. A laboratory-scale system integrating ultraviolet (UV) photodegradation and a biotrickling filter (BTF) was developed to treat dichloromethane (DCM), and this was compared to BTF alone. A combined UV-BTF approach permitted faster biofilm formation and greater removal than BTF. DCM distribution and its photodegradation intermediates revealed that the lower filter of the UV-BTF contributed more to CO2 production; the upper filter assisted more with DCM removal. The UV-BTF kept secretion of extracellular polymeric substances at a normal level with an evenly distributed biomass. Pyrosequencing analysis showed that the dominant population in the combined biofilter was more diverse than that in BTF alone. Our data provide a foundation for understanding the effect of UV pretreatment on BTF performance and the microbial community.