Nana Takahashi1, Fuminori Iwasa2, Yuuki Inoue3, Hirobumi Morisaki4, Kazuhiko Ishihara5, Kazuyoshi Baba6. 1. Assistant Professor, Department of Prosthodontics, School of Dentistry, Showa University, Tokyo, Japan. 2. Lecturer, Department of Prosthodontics, School of Dentistry, Showa University, Tokyo, Japan. 3. Assistant Professor, Department of Materials Engineering, School of Engineering, University of Tokyo, Tokyo, Japan. 4. Lecturer, Department of Oral Microbiology, School of Dentistry, Showa University, Tokyo, Japan. 5. Professor, Department of Materials Engineering, School of Engineering, University of Tokyo, Tokyo, Japan. 6. Professor and Chair, Department of Prosthodontics, School of Dentistry, Showa University, Tokyo, Japan.
Abstract
STATEMENT OF PROBLEM: The polymer 2-methacryloyloxyethyl phosphorylcholine is currently used on medical devices to prevent infection. Denture plaque-associated infection is regarded as a source of serious dental and medical complications in the elderly population, and denture hygiene, therefore, is an issue of considerable importance for denture wearers. Furthermore, because denture bases are exposed to mechanical stresses, for example, denture brushing, the durability of the coating is important for retaining the antiadhesive function of 2-methacryloyloxyethyl phosphorylcholine. PURPOSE: The purpose of this study is to investigate the durability and antiadhesive activity of two 2-methacryloyloxyethyl phosphorylcholine polymer coating techniques: poly-2-methacryloyloxyethyl phosphorylcholine grafting and poly-2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate coating. It was revealed that 2-methacryloyloxyethyl phosphorylcholine polymer coating of the denture base resin polymethyl methacrylate decreases bacterial biofilm formation. MATERIAL AND METHODS: Durability was examined by rhodamine staining and elemental surface analysis and by determining the wetting properties of the 2-methacryloyloxyethyl phosphorylcholine polymer-modified polymethyl methacrylate after a friction test that comprised 500 brushing cycles. Antiadhesive activity was examined by using a Streptococcus mutans biofilm formation assay. RESULTS: Poly-2-methacryloyloxyethyl phosphorylcholine-grafted polymethyl methacrylate retained 2-methacryloyloxyethyl phosphorylcholine units and antiadhesive activity even after repetitive mechanical stress, whereas co-n-butyl methacrylate-coated polymethyl methacrylate did not. CONCLUSION: These results demonstrated that graft polymerization of 2-methacryloyloxyethyl phosphorylcholine on denture surfaces may contribute to the durability of the coating and prevent microbial retention.
STATEMENT OF PROBLEM: The polymer 2-methacryloyloxyethyl phosphorylcholine is currently used on medical devices to prevent infection. Denture plaque-associated infection is regarded as a source of serious dental and medical complications in the elderly population, and denture hygiene, therefore, is an issue of considerable importance for denture wearers. Furthermore, because denture bases are exposed to mechanical stresses, for example, denture brushing, the durability of the coating is important for retaining the antiadhesive function of 2-methacryloyloxyethyl phosphorylcholine. PURPOSE: The purpose of this study is to investigate the durability and antiadhesive activity of two 2-methacryloyloxyethyl phosphorylcholinepolymer coating techniques: poly-2-methacryloyloxyethyl phosphorylcholine grafting and poly-2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate coating. It was revealed that 2-methacryloyloxyethyl phosphorylcholinepolymer coating of the denture base resin polymethyl methacrylate decreases bacterial biofilm formation. MATERIAL AND METHODS: Durability was examined by rhodamine staining and elemental surface analysis and by determining the wetting properties of the 2-methacryloyloxyethyl phosphorylcholinepolymer-modified polymethyl methacrylate after a friction test that comprised 500 brushing cycles. Antiadhesive activity was examined by using a Streptococcus mutans biofilm formation assay. RESULTS:Poly-2-methacryloyloxyethyl phosphorylcholine-grafted polymethyl methacrylate retained 2-methacryloyloxyethyl phosphorylcholine units and antiadhesive activity even after repetitive mechanical stress, whereas co-n-butyl methacrylate-coated polymethyl methacrylate did not. CONCLUSION: These results demonstrated that graft polymerization of 2-methacryloyloxyethyl phosphorylcholine on denture surfaces may contribute to the durability of the coating and prevent microbial retention.
Authors: Ning Zhang; Ke Zhang; Mary A S Melo; Michael D Weir; David J Xu; Yuxing Bai; Hockin H K Xu Journal: Int J Mol Sci Date: 2017-01-18 Impact factor: 5.923
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