Ning Zhang1, Mary Anne S Melo2, Yuxing Bai3, Hockin H K Xu4. 1. Department of Orthodontics, School of Stomatology, Capital Medical University, Beijing, China; Biomaterials & Tissue Engineering Division, Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, MD 21201, USA. 2. Biomaterials & Tissue Engineering Division, Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, MD 21201, USA. 3. Department of Orthodontics, School of Stomatology, Capital Medical University, Beijing, China. Electronic address: byuxing@263.net. 4. Biomaterials & Tissue Engineering Division, Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, MD 21201, USA; Center for Stem Cell Biology & Regenerative Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA; Marlene and Stewart Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimore, MD 21201, USA; Department of Mechanical Engineering, University of Maryland, Baltimore County, MD 21250, USA. Electronic address: hxu@umaryland.edu.
Abstract
OBJECTIVES: Biofilms at tooth-restoration margins can produce acids and cause secondary caries. A protein-repellent adhesive resin can potentially inhibit bacteria attachment and biofilm growth. However, there has been no report on protein-repellent dental resins. The objectives of this study were to develop a protein-repellent bonding agent incorporating 2-methacryloyloxyethyl phosphorylcholine (MPC), and to investigate its resistance to protein adsorption and biofilm growth for the first time. METHODS: MPC was incorporated into Scotchbond Multi-Purpose (SBMP) at 0%, 3.75%, 7.5%, 11.25%, and 15% by mass. Extracted human teeth were used to measure dentine shear bond strengths. Protein adsorption onto resins was determined by a micro bicinchoninic acid (BCA) method. A dental plaque microcosm biofilm model with human saliva as inoculum was used to measure biofilm metabolic activity and colony-forming unit (CFU) counts. RESULTS: Adding 7.5% MPC into primer and adhesive did not decrease the dentine bond strength, compared to control (p>0.1). Incorporation of 7.5% of MPC achieved the lowest protein adsorption, which was 20-fold less than that of control. Incorporation of 7.5% of MPC greatly reduced bacterial adhesion, yielding biofilm total microorganism, total streptococci, and mutans streptococci CFU that were an order of magnitude less than control. CONCLUSIONS: A protein-repellent dental adhesive resin was developed for the first time. Incorporation of MPC into primer and adhesive at 7.5% by mass greatly reduced the protein adsorption and bacterial adhesion, without compromising the dentine bond strength. CLINICAL SIGNIFICANCE: The novel protein-repellent primer and adhesive are promising to inhibit biofilm formation and acid production, to protect the tooth-restoration margins and prevent secondary caries.
OBJECTIVES: Biofilms at tooth-restoration margins can produce acids and cause secondary caries. A protein-repellent adhesive resin can potentially inhibit bacteria attachment and biofilm growth. However, there has been no report on protein-repellent dental resins. The objectives of this study were to develop a protein-repellent bonding agent incorporating 2-methacryloyloxyethyl phosphorylcholine (MPC), and to investigate its resistance to protein adsorption and biofilm growth for the first time. METHODS:MPC was incorporated into Scotchbond Multi-Purpose (SBMP) at 0%, 3.75%, 7.5%, 11.25%, and 15% by mass. Extracted human teeth were used to measure dentine shear bond strengths. Protein adsorption onto resins was determined by a micro bicinchoninic acid (BCA) method. A dental plaque microcosm biofilm model with human saliva as inoculum was used to measure biofilm metabolic activity and colony-forming unit (CFU) counts. RESULTS: Adding 7.5% MPC into primer and adhesive did not decrease the dentine bond strength, compared to control (p>0.1). Incorporation of 7.5% of MPC achieved the lowest protein adsorption, which was 20-fold less than that of control. Incorporation of 7.5% of MPC greatly reduced bacterial adhesion, yielding biofilm total microorganism, total streptococci, and mutans streptococci CFU that were an order of magnitude less than control. CONCLUSIONS: A protein-repellent dental adhesive resin was developed for the first time. Incorporation of MPC into primer and adhesive at 7.5% by mass greatly reduced the protein adsorption and bacterial adhesion, without compromising the dentine bond strength. CLINICAL SIGNIFICANCE: The novel protein-repellent primer and adhesive are promising to inhibit biofilm formation and acid production, to protect the tooth-restoration margins and prevent secondary caries.
Authors: Ning Zhang; Mary A S Melo; Chen Chen; Jason Liu; Michael D Weir; Yuxing Bai; Hockin H K Xu Journal: Dent Mater Date: 2015-07-14 Impact factor: 5.304
Authors: Yuncong Li; Xiaoyi Hu; Yang Xia; Yadong Ji; Jianping Ruan; Michael D Weir; Xiaoying Lin; Zhihong Nie; Ning Gu; Radi Masri; Xiaofeng Chang; Hockin H K Xu Journal: Dent Mater Date: 2018-06-21 Impact factor: 5.304
Authors: Ning Zhang; Mary A S Melo; Michael D Weir; Mark A Reynolds; Yuxing Bai; Hockin H K Xu Journal: Materials (Basel) Date: 2016-11-01 Impact factor: 3.623
Authors: Ning Zhang; Ke Zhang; Xianju Xie; Zixiang Dai; Zeqing Zhao; Satoshi Imazato; Yousif A Al-Dulaijan; Faisal D Al-Qarni; Michael D Weir; Mark A Reynolds; Yuxing Bai; Lin Wang; Hockin H K Xu Journal: Nanomaterials (Basel) Date: 2018-06-01 Impact factor: 5.076