Draft Genome Sequence of Enterococcus faecalis Strain PF3, Isolated from Adelie Penguin Feces from Antarctica.

Enterococcus faecalis is one of the leading causes of nosocomial infections and is a common commensal organism in humans and other animals. In this study, we report a draft genome sequence for the E. faecalis strain PF3, isolated from Adélie penguin feces collected from Warriner Island, Antarctica.

GENOME ANNOUNCEMENT

Enterococcus spp. are Gram-positive bacteria commonly found under a wide range of different environmental conditions (1). Within this genus, Enterococcus faecalis is a common commensal organism found in the gastrointestinal tract of warm-blooded animals (2). This species has been isolated from a range of different organisms, including mammals, birds, food industry animals, insects, and plants (3). Here, we completed a draft genome sequence for E. faecalis strain PF3, isolated from Adélie penguin (Pygoscelis adeliae) feces collected on Warriner Island, Antarctica.

The genome of E. faecalis PF3 was sequenced on an Ion Proton PGM using a P1 chip with 200-bp chemistry. The raw data comprise 921 megabases with 8,723,410 reads averaging 105.6 bp. De novo assembly was performed using CLC Genomics Workbench 6.02, producing 401 contigs, with an N50 of 59,627 bp and with the largest contig being 201,859 bp. The draft genome that resulted from this assembly is 3,215,729 bp long with a G+C content of 37.5%. xBASE annotation (4–9) detected a total of 3,387 genes, of which 3,338 are coding sequences (CDSs), 45 are tRNA genes, and 4 are rRNA genes.

Progressive Mauve (10) was used to align E. faecalis PF3 to other E. faecalis reference genomes. Pairwise alignments determined that the PF3 strain shows the greatest homology to the widespread commensal E. faecalis OG1RF strain. The draft genome contains a pathogenicity island, multiple plasmid sequences, and three clusters of regularly interspaced short palindromic repeat (CRISPR)-associated genes.

Nucleotide sequence accession numbers.

This whole-genome shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession no. AZIA00000000. The version described in this paper is version AZIA01000000.