Literature DB >> 24447137

A technique to increase protein yield in a rabbit reticulocyte lysate translation system.

Maria Anastasina1, Ilya Terenin2, Sarah J Butcher3, Denis E Kainov1.   

Abstract

Rabbit reticulocyte lysate (RRL) is a mammalian cell-free system for protein production. However, one of the limitations of this system is its low protein yield. Inclusion of recombinant virus proteins and specific viral structures on target mRNA could enhance protein production in RRL. Here we show that simultaneous addition of influenza A virus NS1 protein and inclusion of the encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES) in the target mRNA facilitate translation initiation and increase protein yield over 10-fold, improving the translation capacity of RRL.

Entities:  

Keywords:  EMCV IRES; cell-free translation; influenza A virus; non-structural protein; rabbit reticulocyte lysate

Mesh：

Substances：

Year:  2014        PMID： 24447137     DOI： 10.2144/000114125

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  11 in total

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2.  Dissecting limiting factors of the Protein synthesis Using Recombinant Elements (PURE) system.

Authors:  Jun Li; Chi Zhang; Poyi Huang; Erkin Kuru; Eliot T C Forster-Benson; Taibo Li; George M Church
Journal:  Translation (Austin)       Date:  2017-05-09

3.  RNA Modulates the Interaction between Influenza A Virus NS1 and Human PABP1.

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Journal:  Biochemistry       Date:  2018-05-25       Impact factor: 3.162

4.  Influenza A Virus NS1 Protein Binds as a Dimer to RNA-Free PABP1 but Not to the PABP1·Poly(A) RNA Complex.

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Journal:  Cell Mol Life Sci       Date:  2016-11-16       Impact factor: 9.207

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Journal:  Front Mol Biosci       Date:  2021-03-25

Review 8.  Cell-Free Protein Synthesis: Pros and Cons of Prokaryotic and Eukaryotic Systems.

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Journal:  Chembiochem       Date:  2015-10-19       Impact factor: 3.461

Review 9.  Cell-Free Protein Synthesis: A Promising Option for Future Drug Development.

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Journal:  BioDrugs       Date:  2020-06       Impact factor: 5.807

Review 10.  Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology.

Authors:  Nadanai Laohakunakorn; Laura Grasemann; Barbora Lavickova; Grégoire Michielin; Amir Shahein; Zoe Swank; Sebastian J Maerkl
Journal:  Front Bioeng Biotechnol       Date:  2020-03-24
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