| Literature DB >> 24444464 |
Danijela Jelovac1, Julia A Beaver1, Sasidharan Balukrishna2, Hong Yuen Wong1, Patricia Valda Toro1, Ashley Cimino-Mathews1, Pedram Argani1, Vered Stearns1, Lisa Jacobs1, Dustin VanDenBerg1, Jill Kessler1, Stacie Jeter1, Ben H Park3, Antonio C Wolff1.
Abstract
Digital polymerase chain reaction is a new technology that enables detection and quantification of cancer DNA molecules from peripheral blood. Using this technique, we identified mutant PIK3CA DNA in circulating ptDNA (plasma tumor DNA) from a patient with concurrent early stage breast cancer and non-small cell lung cancer. The patient underwent successful resection of both her breast and lung cancers, and using standard Sanger sequencing the breast cancer was shown to harbor the identical PIK3CA mutation identified in peripheral blood. This case report highlights potential applications and concerns that can arise with the use of ptDNA in clinical oncology practice.Entities:
Keywords: Breast cancer; Digital PCR; Lung cancer; PIK3CA; Plasma tumor DNA
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Year: 2013 PMID： 24444464 PMCID： PMC3965626 DOI： 10.1016/j.humpath.2013.10.016
Source DB: PubMed Journal: Hum Pathol ISSN： 0046-8177 Impact factor: 3.466