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Literature DB >> 24443077

Dynamic assessment of cell viability, proliferation and migration using real time cell analyzer system (RTCA).

Mani Roshan Moniri¹, Ada Young, Kelsey Reinheimer, Jarrett Rayat, Long-Jun Dai, Garth L Warnock.

Abstract

Cell viability and cell migration capacities are critical parameters for cell culture-related studies. It is essential to monitor the dynamic changes of cell properties under various co-culture conditions to our better understanding of their behaviours and characteristics. The real time cell analyzer (RTCA, xCELLigence, Roche) is an impedance-based technology that can be used for label-free and real-time monitoring of cell properties, such as cell adherence, proliferation, migration and cytotoxicity. The practicality of this system has been proven in our recent cancer studies. In the present method, we intend to use co-cultures of pancreatic cancer cells (HP62) and mesenchymal stem cells to describe in detail, the procedures and benefits of RTCA.

Entities: Disease

Year: 2014 PMID： 24443077 PMCID： PMC4329294 DOI： 10.1007/s10616-014-9692-5

Source DB: PubMed Journal: Cytotechnology ISSN： 0920-9069 Impact factor: 2.058

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1. Endothelial cell migration assay. A quantitative assay for prediction of in vivo biology.

Authors: Mark W Lingen
Journal: Methods Mol Med Date: 2003

2. An automatic and quantitative on-chip cell migration assay using self-assembled monolayers combined with real-time cellular impedance sensing.

Authors: Lei Wang; Jing Zhu; Cheng Deng; Wan-li Xing; Jing Cheng
Journal: Lab Chip Date: 2008-05-07 Impact factor: 6.799

3. Advanced glycosylation end products in adrenal lipofuscin.

Authors: I Shimokawa; Y Higami; S Horiuchi; M Iwasaki; T Ikeda
Journal: J Gerontol A Biol Sci Med Sci Date: 1998-01 Impact factor: 6.053

4. Osteogenesis in transplants of bone marrow cells.

Authors: A J Friedenstein; I I Piatetzky-Shapiro; K V Petrakova
Journal: J Embryol Exp Morphol Date: 1966-12

5. MSC(TRAIL)-mediated HepG2 cell death in direct and indirect co-cultures.

Authors: Xu-Yong Sun; Jiang Nong; Ke Qin; Hong Lu; Mani R Moniri; Long-Jun Dai; Garth L Warnock
Journal: Anticancer Res Date: 2011-11 Impact factor: 2.480

6. Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry.

Authors: I Schmid; W J Krall; C H Uittenbogaart; J Braun; J V Giorgi
Journal: Cytometry Date: 1992

7. Assessment of therapeutic efficacy and fate of engineered human mesenchymal stem cells for cancer therapy.

Authors: Laura S Sasportas; Randa Kasmieh; Hiroaki Wakimoto; Shawn Hingtgen; Jeroen A J M van de Water; Gayatry Mohapatra; Jose Luiz Figueiredo; Robert L Martuza; Ralph Weissleder; Khalid Shah
Journal: Proc Natl Acad Sci U S A Date: 2009-03-05 Impact factor: 11.205

8. Dual-targeted antitumor effects against brainstem glioma by intravenous delivery of tumor necrosis factor-related, apoptosis-inducing, ligand-engineered human mesenchymal stem cells.

Authors: Bojie Yang; Xing Wu; Ying Mao; Weiming Bao; Liang Gao; Ping Zhou; Rong Xie; Liangfu Zhou; Jianhong Zhu
Journal: Neurosurgery Date: 2009-09 Impact factor: 4.654

9. Fluorescence-based quantitative scratch wound healing assay demonstrating the role of MAPKAPK-2/3 in fibroblast migration.

Authors: Manoj B Menon; Natalia Ronkina; Jessica Schwermann; Alexey Kotlyarov; Matthias Gaestel
Journal: Cell Motil Cytoskeleton Date: 2009-12

10. Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining.

Authors: A Krishan
Journal: J Cell Biol Date: 1975-07 Impact factor: 10.539

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2. Attenuation of Yersinia pestis fyuA Mutants Caused by Iron Uptake Inhibition and Decreased Survivability in Macrophages.

Authors: Yulu Chen; Kai Song; Xin Chen; Ye Li; Ruichen Lv; Qingwen Zhang; Yujun Cui; Yujing Bi; Yanping Han; Yafang Tan; Zongmin Du; Ruifu Yang; Zhizhen Qi; Yajun Song
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3. miR-30c and semaphorin 3A determine adult neurogenesis by regulating proliferation and differentiation of stem cells in the subventricular zones of mouse.

Authors: Tingting Sun; Weiyun Li; Shucai Ling
Journal: Cell Prolif Date: 2016-05-15 Impact factor: 6.831

4. Cell-Surface Glyco-Engineering by Exogenous Enzymatic Transfer Using a Bifunctional CMP-Neu5Ac Derivative.

Authors: Chantelle J Capicciotti; Chengli Zong; M Osman Sheikh; Tiantian Sun; Lance Wells; Geert-Jan Boons
Journal: J Am Chem Soc Date: 2017-09-15 Impact factor: 15.419

5. Cytotoxicity evaluation of five different dual-cured resin cements used for fiber posts cementation.

Authors: M Dioguardi; D Perrone; G Troiano; L Laino; F Ardito; F Lauritano; M Cicciù; L Lo Muzio
Journal: Int J Clin Exp Med Date: 2015-03-07

6. Salinomycin efficiency assessment in non-tumor (HB4a) and tumor (MCF-7) human breast cells.

Authors: Andressa Megumi Niwa; Gláucia Fernanda Rocha D Epiro; Lilian Areal Marques; Simone Cristine Semprebon; Daniele Sartori; Lúcia Regina Ribeiro; Mário Sérgio Mantovani
Journal: Naunyn Schmiedebergs Arch Pharmacol Date: 2016-03-02 Impact factor: 3.000

7. Real-time monitoring of hypertrophy in HL-1 cardiomyocytes by impedance measurements reveals different modes of growth.

Authors: Laura Bloch; Bernadin Ndongson-Dongmo; Angelika Kusch; Duska Dragun; Regine Heller; Otmar Huber
Journal: Cytotechnology Date: 2016-07-05 Impact factor: 2.058

8. Rasfonin, a novel 2-pyrone derivative, induces ras-mutated Panc-1 pancreatic tumor cell death in nude mice.

Authors: Z Xiao; L Li; Y Li; W Zhou; J Cheng; F Liu; P Zheng; Y Zhang; Y Che
Journal: Cell Death Dis Date: 2014-05-22 Impact factor: 8.469

9. Mitofusin 2 Deficiency Affects Energy Metabolism and Mitochondrial Biogenesis in MEF Cells.

Authors: Maria Kawalec; Anna Boratyńska-Jasińska; Małgorzata Beręsewicz; Dorota Dymkowska; Krzysztof Zabłocki; Barbara Zabłocka
Journal: PLoS One Date: 2015-07-31 Impact factor: 3.240

10. Using real-time impedance-based assays to monitor the effects of fibroblast-derived media on the adhesion, proliferation, migration and invasion of colon cancer cells.

Authors: Catríona M Dowling; Carmen Herranz Ors; Patrick A Kiely
Journal: Biosci Rep Date: 2014-07-29 Impact factor: 3.840