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Literature DB >> 24442259

A procedure for the in vivo determination of enzyme activity in higher plant tissue.

Abstract

Rapid freezing of higher plant tissue in liquid nitrogen renders the cells permeable to a wide range of substrate molecules. Tissue permeabilized by repeated freeze-thaw treatment can be used for the measurement of several enzymes. With most of the tissues examined maximum in vivo activities were obtained using a combination of freeze-thaw treatment followed by vacuum infiltration. The activities and properties of enzymes determined with this procedure are very comparable with those obtained for enzymes prepared by conventional extraction procedures.

Entities: Chemical

Year: 1974 PMID： 24442259 DOI： 10.1007/BF00388389

Source DB: PubMed Journal: Planta ISSN： 0032-0935 Impact factor: 4.116

12 in total

4. Inactivation in vivo of glutamine synthetase and NAD-specific glutamate dehydrogenase: its role in the regulation of glutamine synthesis in yeasts.

Authors: A R Ferguson; A P Sims
Journal: J Gen Microbiol Date: 1971-12

3. The effect of aspartate-derived amino acids (lysine, threonine, methionine) on the growth of excised embryos of wheat and barley.

Authors: S W Bright; E A Wood; B J Miflin
Journal: Planta Date: 1978-01 Impact factor: 4.116

4. Sources of reducing power for nitrate reduction in spinach leaves.

Authors: A F Mann; D P Hucklesby; E J Hewitt
Journal: Planta Date: 1978-01 Impact factor: 4.116

5. Kinetics for glutamine-synthetase inhibition by phosphinothricin and measurement of other enzyme activities in situ in isolated asparagus cells using a freeze-thaw technique.

Authors: A R Fraser; S M Ridley
Journal: Planta Date: 1984-07 Impact factor: 4.116

6. The control of glutamine synthetase level in Lemna minor L.

Authors: D Rhodes; G A Rendon; G R Stewart
Journal: Planta Date: 1975-01 Impact factor: 4.116

7. The estimation of phenylalanine ammonia-lyase (PAL)-activity in intact cells of higher plant tissue : I. Parameters of the assay.

Authors: N Amrhein; K H Gödeke; J Gerhardt
Journal: Planta Date: 1976-01 Impact factor: 4.116

7 in total

A procedure for the in vivo determination of enzyme activity in higher plant tissue.

1. A colorimetric determination of blood acetoin.

2. A technique for the assay of enzymes in intact plant cells in the presence of dimethylsulfoxide.

3. Comparison of in vitro and in vivo assays for nitrate reductase in soybean leaves.

4. Inactivation in vivo of glutamine synthetase and NAD-specific glutamate dehydrogenase: its role in the regulation of glutamine synthesis in yeasts.

5. Nitrate reductase assay in intact plant tissues.

6. Modified methods for the determination of carbamyl aspartate.

Review 7. Allosteric regulation of enzyme activity.

8. Purification and properties of alpha-galactosidases from Vicia faba seeds.

9. Generation of reduced nicotinamide adenine dinucleotide for nitrate reduction in green leaves.

10. Changes in phosphatase activity in phosphorus-deficient Spirodela.

1. Regulation of assimilatory nitrate reduction at the level of nitrite in Chlorella fusca.

2. The permeability of electroporated cells and protoplasts of sugar beet.

3. The effect of aspartate-derived amino acids (lysine, threonine, methionine) on the growth of excised embryos of wheat and barley.

4. Sources of reducing power for nitrate reduction in spinach leaves.

5. Kinetics for glutamine-synthetase inhibition by phosphinothricin and measurement of other enzyme activities in situ in isolated asparagus cells using a freeze-thaw technique.

6. The control of glutamine synthetase level in Lemna minor L.

7. The estimation of phenylalanine ammonia-lyase (PAL)-activity in intact cells of higher plant tissue : I. Parameters of the assay.