P Higgs1, M Costa, A Freke, K Papasouliotis. 1. Small Animal Hospital, School of Veterinary Sciences, University of Bristol, Langford, Bristol, BS40 5DU.
Abstract
OBJECTIVES: The AIA-360 (Tosoh Corporation) is an automated immunoassay analyser. The aims of this study were to estimate the precision of thyroxine and cortisol AIA-360 immunoassays in canine and feline samples and to compare the results produced with those obtained by a chemiluminescence analyser (Immulite® 1000, Siemens). METHODS: Blood samples from 240 clinical cases (60 dogs and 60 cats for both thyroxine and cortisol) were analysed using both instruments. RESULTS: Deming regression calculations showed excellent correlation (thyroxine, canine rs = 0 · 94, feline rs = 0 · 97; cortisol, canine rs = 0 · 97, feline rs = 0 · 97). Agreement between the two instruments was examined by Bland-Altman difference plots, which identified wide confidence intervals and outliers for thyroxine (canine n = 6, feline n = 4) and cortisol (canine n = 3, feline n = 4) results. Inter/intra-run precision of the AIA-360 was excellent for both cortisol and thyroxine (coefficients of variation <7%). CLINICAL SIGNIFICANCE: The instrument showed excellent correlation for cortisol and thyroxine in canine and feline samples demonstrating that the AIA-360 can be used in clinical practice. The agreement studies suggest that the results from the AIA-360 cannot be used interchangeably with those generated by the Immulite 1000 and should be interpreted using reference intervals that have been established specific to the AIA-360.
OBJECTIVES: The AIA-360 (Tosoh Corporation) is an automated immunoassay analyser. The aims of this study were to estimate the precision of thyroxine and cortisol AIA-360 immunoassays in canine and feline samples and to compare the results produced with those obtained by a chemiluminescence analyser (Immulite® 1000, Siemens). METHODS: Blood samples from 240 clinical cases (60 dogs and 60 cats for both thyroxine and cortisol) were analysed using both instruments. RESULTS: Deming regression calculations showed excellent correlation (thyroxine, canine rs = 0 · 94, feline rs = 0 · 97; cortisol, canine rs = 0 · 97, feline rs = 0 · 97). Agreement between the two instruments was examined by Bland-Altman difference plots, which identified wide confidence intervals and outliers for thyroxine (canine n = 6, feline n = 4) and cortisol (canine n = 3, feline n = 4) results. Inter/intra-run precision of the AIA-360 was excellent for both cortisol and thyroxine (coefficients of variation <7%). CLINICAL SIGNIFICANCE: The instrument showed excellent correlation for cortisol and thyroxine in canine and feline samples demonstrating that the AIA-360 can be used in clinical practice. The agreement studies suggest that the results from the AIA-360 cannot be used interchangeably with those generated by the Immulite 1000 and should be interpreted using reference intervals that have been established specific to the AIA-360.
Authors: Monique Kerstens; Gaëlle Boulet; Marian Van Kerckhoven; Sofie Clais; Ellen Lanckacker; Peter Delputte; Louis Maes; Paul Cos Journal: Folia Microbiol (Praha) Date: 2015-05-07 Impact factor: 2.099
Authors: Ewan D S Wolff; Graham Bilbrough; George Moore; Lynn Guptill; J Catharine Scott-Moncrieff Journal: J Vet Intern Med Date: 2020-02-03 Impact factor: 3.333