[Structural changes of the plasmalemma during cell wall regeneration of isolated protoplasts from stem callus of Skimmia japonica thunb].

The structure of the plasmalemma of isolated protoplasts from stem callus cultures of Skimmia japonica was investigated during cell wall regeneration by means of freeze-etching and deep-etching. Three of the four theoretically possible views of the membrane-the two corresponding inner fracture faces and the outer surface-were made visible. In all cases structural alterations were found in the arrangement of the particles, which are probably responsible for the synthesis and deposit of the cellulose microfibrils.