BACKGROUND: Our objective was to examine how the gene expression profile of tumor tissue correlates with lymph node metastasis in patients with advanced colorectal adenocarcinoma (CRAC). METHODS: We studied 36 patients (20 men and 16 women, 22-90 years of age) treated for CRAC (classifications of T2, T3, or T4; histological grade of G1 or G2). Amplified tumor mRNA samples were exposed to 20,000 human sequence probes and digitized images of the hybridized samples were analyzed. RESULTS: On average, 2389 probes were detected above the background, with an average correlation R value of 0.19 between data from different patient groups (with or without lymph node invasion, colon or rectal, with or without angio-lymphatic invasion, with or without recurrence). Lymph node metastasis had a statistically significant signature according to Significance Analysis of Microarrays (SAM) and parametric t-tests, with a false discovery rate (FDR)=0.1% and p=0.001, respectively. Cross-correlation of these two tests identified 102 transcripts as being potentially related to node metastases, with fold changes in the range of 2.182-12.960. CONCLUSION: We identified 102 differentially expressed genes related to the presence of lymph node metastases in patients with advanced colorectal cancer.
BACKGROUND: Our objective was to examine how the gene expression profile of tumor tissue correlates with lymph node metastasis in patients with advanced colorectal adenocarcinoma (CRAC). METHODS: We studied 36 patients (20 men and 16 women, 22-90 years of age) treated for CRAC (classifications of T2, T3, or T4; histological grade of G1 or G2). Amplified tumor mRNA samples were exposed to 20,000 human sequence probes and digitized images of the hybridized samples were analyzed. RESULTS: On average, 2389 probes were detected above the background, with an average correlation R value of 0.19 between data from different patient groups (with or without lymph node invasion, colon or rectal, with or without angio-lymphatic invasion, with or without recurrence). Lymph node metastasis had a statistically significant signature according to Significance Analysis of Microarrays (SAM) and parametric t-tests, with a false discovery rate (FDR)=0.1% and p=0.001, respectively. Cross-correlation of these two tests identified 102 transcripts as being potentially related to node metastases, with fold changes in the range of 2.182-12.960. CONCLUSION: We identified 102 differentially expressed genes related to the presence of lymph node metastases in patients with advanced colorectal cancer.
Authors: Ramesh Ramakrishnan; David Dorris; Anna Lublinsky; Allen Nguyen; Marc Domanus; Anna Prokhorova; Linn Gieser; Edward Touma; Randall Lockner; Murthy Tata; Xiaomei Zhu; Marcus Patterson; Richard Shippy; Timothy J Sendera; Abhijit Mazumder Journal: Nucleic Acids Res Date: 2002-04-01 Impact factor: 16.971
Authors: Dennis A Benson; Ilene Karsch-Mizrachi; David J Lipman; James Ostell; David L Wheeler Journal: Nucleic Acids Res Date: 2004-01-01 Impact factor: 16.971
Authors: Aurélien de Reyniès; Daniela Geromin; Jean-Michel Cayuela; Fabien Petel; Philippe Dessen; François Sigaux; David S Rickman Journal: BMC Genomics Date: 2006-03-15 Impact factor: 3.969