Literature DB >> 24426156

Cloning and Expression of Genes Encoding F107-C and K88-1NT Fimbrial Proteins of Enterotoxigenic Escherichia coli from Piglets.

Nguyen Hoang Loc1, Le My Tieu Ngoc1, Tran Thuy Lan1, Le Quoc Viet1, Le Duc Thao1, Hoang Tan Quang1, Dinh Thi Bich Lan1, Phung Thang Long2.   

Abstract

We cloned two genes coding F107-C and K88-1NT fimbrial subunits from strains E. coli C and 1NT isolated from Thua Thien Hue province, Vietnam. The mature peptide of faeG gene from strain E. coli 1NT (called faeG-1NT) is 100 % similarity with faeG gene, while the CDS of fedA gene from strain C (called fedA-C) has a similarity of 97 % with the fedA gene. Expression of the faeG-1NT and fedA-C genes in E. coli BL21 Star™ (DE3) produced proteins of ~31 and 22 kDa, respectively. The effect of IPTG concentration on the K88-1NT and F107-C fimbriae production was investigated. The results showed that 0.5 mM IPTG is suitable for higher expression of K88-1NT subunit, while 0.75 mM IPTG strongly stimulated expression of F107-C subunit. The optimal induction time for expression was also examined. Generally, highest expression of K88-1NT subunit occurred after 6 h of induction, while that of F107-C subunit is after 14 h.

Entities:  

Keywords:  E. coli; F107-C; Fimbriae; K88-1NT; faeG; fedA

Year:  2013        PMID： 24426156      PMCID： PMC3779301          DOI： 10.1007/s12088-013-0386-z

Source DB:  PubMed          Journal:  Indian J Microbiol        ISSN： 0046-8991            Impact factor:   2.461


  12 in total

1.  Production of FaeG, the major subunit of K88 fimbriae, in transgenic tobacco plants and its immunogenicity in mice.

Authors:  Yahong Huang; Wanqi Liang; Aihu Pan; Zhiai Zhou; Cheng Huang; Jianxiu Chen; Dabing Zhang
Journal:  Infect Immun       Date:  2003-09       Impact factor: 3.441

2.  Characterization of the antigenic and adhesive properties of FaeG, the major subunit of K88 fimbriae.

Authors:  D Bakker; P T Willemsen; L H Simons; F G van Zijderveld; F K de Graaf
Journal:  Mol Microbiol       Date:  1992-01       Impact factor: 3.501

3.  Pathogenicity of porcine enterotoxigenic Escherichia coli that do not express K88, K99, F41, or 987P adhesins.

Authors:  T A Casey; B Nagy; H W Moon
Journal:  Am J Vet Res       Date:  1992-09       Impact factor: 1.156

4.  Cloning of DNA sequences encoding foreign peptides and their expression in the K88 pili.

Authors:  G Thiry; A Clippe; T Scarcez; J Petre
Journal:  Appl Environ Microbiol       Date:  1989-04       Impact factor: 4.792

5.  Characterization of F18 fimbrial genes fedE and fedF involved in adhesion and length of enterotoxemic Escherichia coli strain 107/86.

Authors:  H Imberechts; P Wild; G Charlier; H De Greve; P Lintermans; P Pohl
Journal:  Microb Pathog       Date:  1996-09       Impact factor: 3.738

6.  Fimbriae and enterotoxins associated with Escherichia coli serogroups isolated from pigs with colibacillosis.

Authors:  R A Wilson; D H Francis
Journal:  Am J Vet Res       Date:  1986-02       Impact factor: 1.156

7.  Characterization of the adhesin of Escherichia coli F18 fimbriae.

Authors:  A Smeds; K Hemmann; M Jakava-Viljanen; S Pelkonen; H Imberechts; A Palva
Journal:  Infect Immun       Date:  2001-12       Impact factor: 3.441

8.  Protective immune response of bacterially-derived recombinant FaeG in piglets.

Authors:  Huang Yahong; Wanqi Liang; Aihu Pan; Zhiai Zhou; Qiang Wang; Cheng Huang; Jianxiu Chen; Dabing Zhang
Journal:  J Microbiol       Date:  2006-10       Impact factor: 3.422

9.  Secretory expression of K88 (F4) fimbrial adhesin FaeG by recombinant Lactococcus lactis for oral vaccination and its protective immune response in mice.

Authors:  Chun Xia Hu; Zi Rong Xu; Wei Fen Li; Dong Niu; Niu Dong; Ping Lu; Ling Lin Fu
Journal:  Biotechnol Lett       Date:  2009-03-10       Impact factor: 2.461

10.  Oral immunization of piglets with recombinant F4 fimbrial adhesin FaeG monomers induces a mucosal and systemic F4-specific immune response.

Authors:  F Verdonck; E Cox; Y Van der Stede; B M Goddeeris
Journal:  Vaccine       Date:  2004-10-22       Impact factor: 3.641
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  1 in total

1.  Functional expression of the entire adhesiome of Salmonella enterica serotype Typhimurium.

Authors:  Nicole Hansmeier; Katarzyna Miskiewicz; Laura Elpers; Viktoria Liss; Michael Hensel; Torsten Sterzenbach
Journal:  Sci Rep       Date:  2017-09-04       Impact factor: 4.379
  1 in total

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