Literature DB >> 24423267

Pulse-chase analysis to measure protein degradation.

Einav Simon1, Daniel Kornitzer2.   

Abstract

In order to measure protein half-life by pulse-chase analysis, cells are incubated with labeled precursors, [(35)S]-methionine and [(35)S]-cysteine, for a short time (pulse), after which they are washed from the labeled compound and incubated with excess cold precursor (chase). The decay of the amount of protein labeled during the pulse period is measured during the chase with cold precursor.
© 2014 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Cellular proteins; Cytoplasmic pools; Derivation of protein half-life; Immunoprecipitation; Methionine pulse-chase; Protein degradation; Pulse-chase analysis

Mesh：

Substances：

Year:  2014        PMID： 24423267     DOI： 10.1016/B978-0-12-420070-8.00006-4

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  10 in total

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