Sources of microorganisms in pozol, a traditional Mexican fermented maize dough.

Freshly prepared pozol, a traditional Mexican fermented maize dough, contained (c.f.u./g wet wt): lactic acid bacteria, 10(4) to 10(6); aerobic mesophiles, 10(4) to 10(5); Enterobacteriaceae, 10(2) to 10(3); yeasts, 10(2) to 10(4); and mould propagules, <10(3). After 30 h at 28°C the numbers were, respectively: 10(9), 7×10(6), 5×10(5), 10(6) and 10(4). Soaking alkali-treated grains overnight allowed lactic acid bacteria, aerobic mesophiles and Enterobacteriaceae to grow and these then constituted the primary microbial flora of the pozol dough. Grinding in a commercial mill inoculated the dough with lactic acid bacteria, aerobic mesophiles, Enterobacteriaceae and yeasts. Other processing stages, including the nature of the surface upon which the balls were made, handling of the dough, and air, contributed only minor numbers of microbes compared with the two major sources, soaking and grinding. The pH of pozol fell from an initial value of 7.3 to 4.6 after 30 h incubation at 28°C. The numbers of Enterobacteriaceae and other aerobic mesophilic bacteria remained constant between 11 and 30 h incubation and there was no evidence of the acidic conditions having any lethal effects on these organisms.