Literature DB >> 24414902

Purification of Neurospora crassa alkaline phosphatase without DNAse activity for use in molecular biology.

M Pereira1, H Pereira, G Thedei, A Rossi, N M Martinez-Rossi.   

Abstract

Alkaline phosphatase, excreted by Neurospora crassa preg (c) and purified to apparent homogeneity by 7.5% PAGE, did not show DNAase activity and removed the terminal 5'-phosphate group from plasmid Bluescript M13(+) linearized with EcoRI. The preg (c) strain may therefore replace other sources of alkaline phosphatase for use in dephosphorylating linearized plasmidial DNA in cloning experiments.

Entities:  

Year:  1995        PMID： 24414902     DOI： 10.1007/BF00286362

Source DB:  PubMed          Journal:  World J Microbiol Biotechnol        ISSN： 0959-3993            Impact factor:   3.312


  6 in total

1.  DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors:  B J DAVIS
Journal:  Ann N Y Acad Sci       Date:  1964-12-28       Impact factor: 5.691

2.  Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone.

Authors:  P H Seeburg; J Shine; J A Martial; J D Baxter; H M Goodman
Journal:  Nature       Date:  1977-12-08       Impact factor: 49.962

3.  Determination of protein: a modification of the Lowry method that gives a linear photometric response.

Authors:  E F Hartree
Journal:  Anal Biochem       Date:  1972-08       Impact factor: 3.365

4.  Genetic control of alkaline phosphatase synthesis in Neurospora: the use of partial diploids in dominance studies.

Authors:  R L Metzenberg; M K Gleason; B S Littlewood
Journal:  Genetics       Date:  1974-05       Impact factor: 4.562

5.  Rat insulin genes: construction of plasmids containing the coding sequences.

Authors:  A Ullrich; J Shine; J Chirgwin; R Pictet; E Tischer; W J Rutter; H M Goodman
Journal:  Science       Date:  1977-06-17       Impact factor: 47.728

6.  Insertional mutagenesis in Neurospora crassa: cloning and molecular analysis of the preg+ gene controlling the activity of the transcriptional activator NUC-1.

Authors:  S Kang; R L Metzenberg
Journal:  Genetics       Date:  1993-02       Impact factor: 4.562
  6 in total
  3 in total

1.  Extracellular alkaline phosphatase from the filamentous fungus Aspergillus caespitosus: purification and biochemical characterization.

Authors:  L H Guimarães; H F Terenzi; J A Jorge; F A Leone; M L Polizeli
Journal:  Folia Microbiol (Praha)       Date:  2003       Impact factor: 2.099

2.  Purification and biochemical characterization of thermostable alkaline phosphatases produced by Rhizopus microsporus var. rhizopodiformis.

Authors:  A Barbosa Junior; L H S GuimarAes; H F Terenzi; J A Jorge; F A Leone; M L T M Polizeli
Journal:  Folia Microbiol (Praha)       Date:  2009-04-18       Impact factor: 2.099

3.  Purification and biochemical characterization of a mycelial alkaline phosphatase without DNAase activity produced by Aspergillus caespitosus.

Authors:  L H S Guimarães; A B Júnior; J A Jorge; H F Terenzi; M L T M Polizeli
Journal:  Folia Microbiol (Praha)       Date:  2007       Impact factor: 2.629
  3 in total

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