Factors affecting formation of micronucleus-like structures after colchicine treatment of Trichoderma reesei.

Micronucleus-like structures were produced in Trichoderma reesei only when 0.1% colchicine treatment was used to enhance nuclear division. The average DNA content of these 'small nuclei' was 30% that of the normal nuclei, indicating that they were aneuploid nuclei. Such small nuclei may be useful in transferring small amounts of DNA into protoplasts.