Literature DB >> 24414513

Variation of RAPD-fingerprint patterns using different DNA-extraction methods with Gram-positive bacteria.

Y Abed1, A Davin, R N Charrel, C Bollet, P De Micco.   

Abstract

The effect of the DNA-extraction method used on fingerprint patterns of RAPD was studied usingStaphylococcus epidermidis andStreptococcus faecalis. The three methods tested (Chelex, microwave and phenol/chloroform) led to significantly different RAPD patterns. The microwave technique generated reproducible patterns and seems the most suitable for RAPD analysis of Gram-positive bacteria.

Entities:  

Year:  1995        PMID： 24414513     DOI： 10.1007/BF00704659

Source DB:  PubMed          Journal:  World J Microbiol Biotechnol        ISSN： 0959-3993            Impact factor:   3.312


  8 in total

1.  Typing of Listeria strains by random amplification of polymorphic DNA.

Authors:  S I Mazurier; K Wernars
Journal:  Res Microbiol       Date:  1992-06       Impact factor: 3.992

2.  Genotypic identification and characterization of species and strains within the genus Candida by using random amplified polymorphic DNA.

Authors:  P F Lehmann; D Lin; B A Lasker
Journal:  J Clin Microbiol       Date:  1992-12       Impact factor: 5.948

3.  A simple method for the isolation of chromosomal DNA from gram positive or acid-fast bacteria.

Authors:  C Bollet; M J Gevaudan; X de Lamballerie; C Zandotti; P de Micco
Journal:  Nucleic Acids Res       Date:  1991-04-25       Impact factor: 16.971

4.  A one-step microbial DNA extraction method using "Chelex 100" suitable for gene amplification.

Authors:  X de Lamballerie; C Zandotti; C Vignoli; C Bollet; P de Micco
Journal:  Res Microbiol       Date:  1992-10       Impact factor: 3.992

5.  Different thermostable DNA polymerases may amplify different RAPD products.

Authors:  B Schierwater; A Ender
Journal:  Nucleic Acids Res       Date:  1993-09-25       Impact factor: 16.971

6.  Randomly amplified polymorphic DNA fingerprinting using combinations of oligonucleotide primers.

Authors:  M R Micheli; R Bova; P Calissano; E D'Ambrosio
Journal:  Biotechniques       Date:  1993-09       Impact factor: 1.993

7.  Fingerprinting genomes using PCR with arbitrary primers.

Authors:  J Welsh; M McClelland
Journal:  Nucleic Acids Res       Date:  1990-12-25       Impact factor: 16.971

8.  Genomic fingerprinting of "Haemophilus somnus" isolates by using a random-amplified polymorphic DNA assay.

Authors:  L E Myers; S V Silva; J D Procunier; P B Little
Journal:  J Clin Microbiol       Date:  1993-03       Impact factor: 5.948
  8 in total

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