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Literature DB >> 2441081

Characterization of a cDNA clone corresponding to a transcript from the Epstein-Barr virus BamHI M fragment: evidence for overlapping mRNAs.

A J Pfitzner, J L Strominger, S H Speck.

Abstract

A 1.95-kilobase cDNA clone was isolated by screening a size-selected lambda gt10 cDNA library prepared from an Epstein-Barr virus-transformed B-cell line, IB4, with the Epstein-Barr virus BamHI M fragment. Sequence analysis revealed that this clone contains about 75% of the BMRF1 and the complete BMRF2 open reading frames. The transcript is not spliced, and the polyadenylation signal at base pair 2641 of the BamHI M fragment is used. Northern blots (RNA blots) indicate that this polyadenylation signal is used for three overlapping mRNAs. The sizes of these transcripts are 3.5, 2.6, and 1.5 kilobases.

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Year: 1987 PMID： 2441081 PMCID： PMC255831

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

23 in total

1. Persisting oncogenic herpesvirus induced by the tumour promotor TPA.

Authors: H zur Hausen; F J O'Neill; U K Freese; E Hecker
Journal: Nature Date: 1978-03-23 Impact factor: 49.962

2. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.

Authors: H Lehrach; D Diamond; J M Wozney; H Boedtker
Journal: Biochemistry Date: 1977-10-18 Impact factor: 3.162

3. An Epstein-Barr virus transcript from a latently infected, growth-transformed B-cell line encodes a highly repetitive polypeptide.

Authors: S H Speck; A Pfitzner; J L Strominger
Journal: Proc Natl Acad Sci U S A Date: 1986-12 Impact factor: 11.205

10. Mta has properties of an RNA export protein and increases cytoplasmic accumulation of Epstein-Barr virus replication gene mRNA.

Authors: O J Semmes; L Chen; R T Sarisky; Z Gao; L Zhong; S D Hayward
Journal: J Virol Date: 1998-12 Impact factor: 5.103

Characterization of a cDNA clone corresponding to a transcript from the Epstein-Barr virus BamHI M fragment: evidence for overlapping mRNAs.

1. Persisting oncogenic herpesvirus induced by the tumour promotor TPA.

2. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.

3. An Epstein-Barr virus transcript from a latently infected, growth-transformed B-cell line encodes a highly repetitive polypeptide.

4. Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping.

5. DNA sequence and expression of the B95-8 Epstein-Barr virus genome.

6. A simple and very efficient method for generating cDNA libraries.

7. Two Epstein-Barr viral nuclear neoantigens distinguished by gene transfer, serology, and chromosome binding.

8. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

9. Epstein-Barr virus RNA. V. Viral RNA in a restringently infected, growth-transformed cell line.

10. DNA sequencing with chain-terminating inhibitors.

1. Identification of a protein encoded in the EB-viral open reading frame BMRF2.

2. Domains of the Epstein-Barr virus (EBV) transcription factor R required for dimerization, DNA binding and activation.

3. Gene mapping and expression of two immunodominant Epstein-Barr virus capsid proteins.

4. The enhancer factor R of Epstein-Barr virus (EBV) is a sequence-specific DNA binding protein.

5. Identification of proteins encoded by Epstein-Barr virus trans-activator genes.

6. Regulation of Epstein-Barr virus promoters in oral epithelial cells and lymphocytes.

7. Transcriptional patterns of the pCD41 (U27) locus of human herpesvirus 6.

8. Identification, characterization, and sequence analysis of a cDNA encoding a phosphoprotein of human herpesvirus 6.

9. A new Epstein-Barr virus transactivator, R, induces expression of a cytoplasmic early antigen.

10. Mta has properties of an RNA export protein and increases cytoplasmic accumulation of Epstein-Barr virus replication gene mRNA.