Serbulent Yigit1, Ahmet Inanir2, Akın Tekcan3, Ercan Tural4, Gokhan Tuna Ozturk2, Gorkem Kismali5, Nevin Karakus1. 1. Gaziosmanpasa University, Faculty of Medicine, Department of Medical Biology, Tokat, Turkey. 2. Gaziosmanpasa University, Faculty of Medicine, Department of Physical Therapy and Rehabilitation, Tokat, Turkey. 3. Ondokuz Mayis University, Faculty of Medicine, Samsun, Turkey. Electronic address: akintekcan@hotmail.com. 4. Ondokuz Mayis University, Faculty of Medicine, Department of Physical Therapy and Rehabilitation, Samsun, Turkey. 5. Ankara University, Faculty of Veterinary Medicine, Department of Biochemistry, Ankara, Turkey.
Abstract
OBJECTIVE: Interleukin-4 (IL-4) is a strong chondroprotective cytokine and polymorphisms within this gene may be a risk factor for osteoarthritis (OA). We aimed to investigate genotype and allele frequencies of IL-4 gene intron 3 variable number of tandem repeats (VNTR) polymorphism in patients with knee OA in a Turkish population. METHODS: The study included 202 patients with knee OA and 180 healthy controls. Genomic DNA was isolated and IL-4 gene 70 bp VNTR polymorphism determined by using polymerase chain reaction (PCR) with specific primers followed by restriction fragment length polymorphism (RFLP) analysis. RESULTS: Our result show that there was statistically significant difference between knee OA patients and control group with respect to IL-4 genotype distribution and allele frequencies (p=0.000, OR: 0.20, 95% CI: 0.10-0.41, OR: 0.22, 95% CI: 0.12-0.42, respectively). CONCLUSIONS: Our findings suggest that there is an association of IL-4 gene intron 3 VNTR polymorphism with susceptibility of a person for development of knee OA. As a result, IL-4 gene intron 3 VNTR polymorphism could be a genetic marker in OA in a Turkish study population. This is the first association study that evaluates the associations between IL-4 gene VNTR polymorphism and knee OA. Crown
OBJECTIVE:Interleukin-4 (IL-4) is a strong chondroprotective cytokine and polymorphisms within this gene may be a risk factor for osteoarthritis (OA). We aimed to investigate genotype and allele frequencies of IL-4 gene intron 3 variable number of tandem repeats (VNTR) polymorphism in patients with knee OA in a Turkish population. METHODS: The study included 202 patients with knee OA and 180 healthy controls. Genomic DNA was isolated and IL-4 gene 70 bp VNTR polymorphism determined by using polymerase chain reaction (PCR) with specific primers followed by restriction fragment length polymorphism (RFLP) analysis. RESULTS: Our result show that there was statistically significant difference between knee OA patients and control group with respect to IL-4 genotype distribution and allele frequencies (p=0.000, OR: 0.20, 95% CI: 0.10-0.41, OR: 0.22, 95% CI: 0.12-0.42, respectively). CONCLUSIONS: Our findings suggest that there is an association of IL-4 gene intron 3 VNTR polymorphism with susceptibility of a person for development of knee OA. As a result, IL-4 gene intron 3 VNTR polymorphism could be a genetic marker in OA in a Turkish study population. This is the first association study that evaluates the associations between IL-4 gene VNTR polymorphism and knee OA. Crown
Authors: Mamdooh Abdullah Gari; Mohammed AlKaff; Haneen S Alsehli; Ashraf Dallol; Abdullah Gari; Muhammad Abu-Elmagd; Roaa Kadam; Mohammed F Abuzinadah; Mazin Gari; Adel M Abuzenadah; Kalamegam Gauthaman; Heba Alkhatabi; Mohammed M Abbas Journal: BMC Med Genet Date: 2016-10-10 Impact factor: 2.103