Literature DB >> 2439440

Preparation and characterization of monoclonal antibodies directed against antigenic determinants of recombinant human tumour necrosis factor (rTNF).

A Meager, S Parti, H Leung, E Peil, B Mahon.   

Abstract

A large number of monoclonal antibodies (McAb) binding to antigenic determinants of human tumour necrosis factor (TNF) were prepared from two fusions of mouse myeloma NSO cells with spleen cells from Balb/c mice immunized with highly purified recombinant (r)TNF. Several of these McAbs were highly neutralizing with respect to the biological activity (cytotoxicity) of TNF manifested in L-929 C1.10 cells. Antibody competition experiments suggested the presence of at least two antigenic determinants on the rTNF molecule through which binding of McAb effects neutralization of biological activity. Some of these McAbs were shown to be suitable for the development of immunoassays to quantify rTNF.

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Year:  1987        PMID： 2439440     DOI： 10.1089/hyb.1987.6.305

Source DB:  PubMed          Journal:  Hybridoma        ISSN： 0272-457X


  27 in total

1.  Monoclonal antibody TGN1412 trial failure explained by species differences in CD28 expression on CD4+ effector memory T-cells.

Authors:  D Eastwood; L Findlay; S Poole; C Bird; M Wadhwa; M Moore; C Burns; R Thorpe; R Stebbings
Journal:  Br J Pharmacol       Date:  2010-10       Impact factor: 8.739

2.  Cytotoxic and noncytotoxic mechanisms involved in the in vitro anti-leukaemia effects of T cell clones established from a chronic myelogenous leukaemia patient during treatment in vivo with interferon alpha.

Authors:  G Pawelec; M Reutter; M Owsianowsky; A Rehbein; F W Busch
Journal:  Cancer Immunol Immunother       Date:  1991       Impact factor: 6.968

3.  Viral infection induces cytokine release by beta islet cells.

Authors:  M G Cavallo; M G Baroni; A Toto; A J Gearing; T Forsey; D Andreani; R Thorpe; P Pozzilli
Journal:  Immunology       Date:  1992-04       Impact factor: 7.397

4.  Elevated serum levels of macrophage-derived cytokines precede and accompany the onset of IDDM.

Authors:  M J Hussain; M Peakman; H Gallati; S S Lo; M Hawa; G C Viberti; P J Watkins; R D Leslie; D Vergani
Journal:  Diabetologia       Date:  1996-01       Impact factor: 10.122

Review 5.  Pharmaceutical aspects of anti-inflammatory TNF-blocking drugs.

Authors:  Sandhya Jinesh
Journal:  Inflammopharmacology       Date:  2015-02-17       Impact factor: 4.473

6.  Release of cytokines during generation of lymphokine-activated killer (LAK) cells by IL-2.

Authors:  G A Limb; A Meager; J Woolley; M Wadhwa; J Biggerstaff; K A Brown; R A Wolstencroft
Journal:  Immunology       Date:  1989-12       Impact factor: 7.397

7.  Alveolar macrophages in AIDS patients: increased spontaneous tumour necrosis factor-alpha production in Pneumocystis carinii pneumonia.

Authors:  V L Krishnan; A Meager; D M Mitchell; A J Pinching
Journal:  Clin Exp Immunol       Date:  1990-05       Impact factor: 4.330

8.  Reduction of tumor necrosis factor alpha and interleukin-1 beta levels in human synovial tissue by interleukin-4 and glucocorticoid.

Authors:  A Bendrups; A Hilton; A Meager; J A Hamilton
Journal:  Rheumatol Int       Date:  1993       Impact factor: 2.631

9.  Cytokines in sera from insulin-dependent diabetic patients at diagnosis.

Authors:  M G Cavallo; P Pozzilli; C Bird; M Wadhwa; A Meager; N Visalli; A J Gearing; D Andreani; R Thorpe
Journal:  Clin Exp Immunol       Date:  1991-11       Impact factor: 4.330

10.  IL-4 acts as a homeostatic regulator of IL-2-induced TNF and IFN-gamma.

Authors:  C Bello-Fernandez; P Oblakowski; A Meager; A S Duncombe; D M Rill; A V Hoffbrand; M K Brenner
Journal:  Immunology       Date:  1991-02       Impact factor: 7.397
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