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Literature DB >> 24390247

FRET-FLIM applications in plant systems.

Christoph A Bücherl¹, Arjen Bader, Adrie H Westphal, Sergey P Laptenok, Jan Willem Borst.

Abstract

A hallmark of cellular processes is the spatio-temporally regulated interplay of biochemical components. Assessing spatial information of molecular interactions within living cells is difficult using traditional biochemical methods. Developments in green fluorescent protein technology in combination with advances in fluorescence microscopy have revolutionised this field of research by providing the genetic tools to investigate the spatio-temporal dynamics of biomolecules in live cells. In particular, fluorescence lifetime imaging microscopy (FLIM) has become an inevitable technique for spatially resolving cellular processes and physical interactions of cellular components in real time based on the detection of Förster resonance energy transfer (FRET). In this review, we provide a theoretical background of FLIM as well as FRET-FLIM analysis. Furthermore, we show two cases in which advanced microscopy applications revealed many new insights of cellular processes in living plant cells as well as in whole plants.

Mesh：

Substances：

Year: 2014 PMID： 24390247 DOI： 10.1007/s00709-013-0595-7

Source DB: PubMed Journal: Protoplasma ISSN： 0033-183X Impact factor: 3.356

59 in total

1. Fluorescence lifetime imaging by time-correlated single-photon counting.

Authors: W Becker; A Bergmann; M A Hink; K König; K Benndorf; C Biskup
Journal: Microsc Res Tech Date: 2004-01-01 Impact factor: 2.769

Review 2. Evanescent-wave field imaging: an introduction to total internal reflection fluorescence microscopy.

Authors: Bryan A Millis
Journal: Methods Mol Biol Date: 2012

3. Combining ocFLIM and FIDSAM reveals fast and dynamic physiological responses at subcellular resolution in living plant cells.

Authors: K Elgass; K Caesar; K Harter; A J Meixner; F Schleifenbaum
Journal: J Microsc Date: 2010-11-18 Impact factor: 1.758

4. Global analysis of time correlated single photon counting FRET-FLIM data.

Authors: Hernan E Grecco; Pedro Roda-Navarro; Peter J Verveer
Journal: Opt Express Date: 2009-04-13 Impact factor: 3.894

5. Fluorescence intensity decay shape analysis microscopy (FIDSAM) for quantitative and sensitive live-cell imaging: a novel technique for fluorescence microscopy of endogenously expressed fusion-proteins.

Authors: Frank Schleifenbaum; Kirstin Elgass; Marcus Sackrow; Katharina Caesar; Kenneth Berendzen; Alfred J Meixner; Klaus Harter
Journal: Mol Plant Date: 2009-12-28 Impact factor: 13.164

Review 6. Green fluorescent protein: applications in cell biology.

Authors: H H Gerdes; C Kaether
Journal: FEBS Lett Date: 1996-06-24 Impact factor: 4.124

7. Measuring FRET using time-resolved FLIM.

Authors: Penny E Morton; Maddy Parsons
Journal: Methods Mol Biol Date: 2011

8. A modified phasor approach for analyzing time-gated fluorescence lifetime images.

Authors: F Fereidouni; A Esposito; G A Blab; H C Gerritsen
Journal: J Microsc Date: 2011-09-20 Impact factor: 1.758

9. Homo-FRET imaging enables quantification of protein cluster sizes with subcellular resolution.

Authors: Arjen N Bader; Erik G Hofman; Jarno Voortman; Paul M P van Bergen en Henegouwen; Hans C Gerritsen
Journal: Biophys J Date: 2009-11-04 Impact factor: 4.033

10. Fluorescence fluctuation analysis of Arabidopsis thaliana somatic embryogenesis receptor-like kinase and brassinosteroid insensitive 1 receptor oligomerization.

Authors: Mark A Hink; Khalid Shah; Eugenia Russinova; Sacco C de Vries; Antonie J W G Visser
Journal: Biophys J Date: 2007-09-28 Impact factor: 4.033

11 in total

10. Combined two-photon excitation and d→f energy transfer in a water-soluble Ir(III)/Eu(III) dyad: two luminescence components from one molecule for cellular imaging.

Authors: Elizabeth Baggaley; Deng-Ke Cao; Daniel Sykes; Stanley W Botchway; Julia A Weinstein; Michael D Ward
Journal: Chemistry Date: 2014-06-16 Impact factor: 5.236

FRET-FLIM applications in plant systems.

1. Fluorescence lifetime imaging by time-correlated single-photon counting.

Review 2. Evanescent-wave field imaging: an introduction to total internal reflection fluorescence microscopy.

3. Combining ocFLIM and FIDSAM reveals fast and dynamic physiological responses at subcellular resolution in living plant cells.

4. Global analysis of time correlated single photon counting FRET-FLIM data.

5. Fluorescence intensity decay shape analysis microscopy (FIDSAM) for quantitative and sensitive live-cell imaging: a novel technique for fluorescence microscopy of endogenously expressed fusion-proteins.

Review 6. Green fluorescent protein: applications in cell biology.

7. Measuring FRET using time-resolved FLIM.

8. A modified phasor approach for analyzing time-gated fluorescence lifetime images.

9. Homo-FRET imaging enables quantification of protein cluster sizes with subcellular resolution.

10. Fluorescence fluctuation analysis of Arabidopsis thaliana somatic embryogenesis receptor-like kinase and brassinosteroid insensitive 1 receptor oligomerization.

1. A novel trimeric complex in plant cells that contributes to the lamina inclination of rice.

Review 2. Techniques for the Analysis of Protein-Protein Interactions in Vivo.

3. Visualizing Protein Associations in Living Arabidopsis Embryo.

4. Coordinated Activation of ARF1 GTPases by ARF-GEF GNOM Dimers Is Essential for Vesicle Trafficking in Arabidopsis.

5. Enrichment of rare events using a multi-parameter high throughput microfluidic droplet sorter.

6. Binary 2in1 Vectors Improve in Planta (Co)localization and Dynamic Protein Interaction Studies.

7. Tracking of fluorescent antibiotic conjugate in planta utilizing fluorescence lifetime imaging.

Review 8. Visualization of in vivo protein-protein interactions in plants.

9. Multi-component quantitative magnetic resonance imaging by phasor representation.

10. Combined two-photon excitation and d→f energy transfer in a water-soluble Ir(III)/Eu(III) dyad: two luminescence components from one molecule for cellular imaging.