Weixian Chen1, Jun Zhang2, Gang Lu2, Zuowei Yuan3, Qian Wu1, Jingjing Li4, Guiping Xu4, An He3, Jian Zheng3, Juan Zhang5. 1. The Clinical Laboratory Center, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China. 2. Artron BioResearch Inc., Burnaby, British Columbia, Canada. 3. The Institute for Viral Hepatitis, Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China. 4. The Blood Transfusion Department, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China. 5. The Blood Transfusion Department, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China. Electronic address: jian0102@hotmail.com.
Abstract
OBJECTIVES: Cholera is an acute malignant infectious disease caused by the bacteria Vibrio cholerae leading to severe dehydrating diarrhea and vomiting, even high rates of mortality in some cases. However, the prevention of the epidemic disease is achievable if proper sanitation practices are followed, provided the accurate and prompt diagnosis of each prevalent serotype in cholera epidemic. The current gold standard of bacterial culture is inadequate for rapid diagnosis. Our aim is to develop an immunochromatographic test format for O1 serotype Ogawa diagnosis and provide the need for better epidemic prevention and early response. DESIGN AND METHODS: The monoclonal antibodies were raised in conventional method and subsequently screened for a match pair. A variety of related and unrelated bacteria strains recruited were employed to test their sensitivity, specificity etc. by indirect ELISA. The human fecal samples were used to test the final lateral-flow device product to satisfy the measurement requirement. RESULTS: A new monoclonal antibody (McAb) pair, named IXiao₃G₆ and IXiao₁D₉, was generated, which is specifically against V. cholerae O1 serotype Ogawa. Additionally, we developed an immunochromatographic lateral flow device (LFD) using this McAb pair for the highly specific and rapid (5 min) detection of Ogawa. CONCLUSIONS: Our product has advantages of simplicity and precision, and can benefit the scene and elementary medical institutions.
OBJECTIVES: Cholera is an acute malignant infectious disease caused by the bacteria Vibrio cholerae leading to severe dehydrating diarrhea and vomiting, even high rates of mortality in some cases. However, the prevention of the epidemic disease is achievable if proper sanitation practices are followed, provided the accurate and prompt diagnosis of each prevalent serotype in cholera epidemic. The current gold standard of bacterial culture is inadequate for rapid diagnosis. Our aim is to develop an immunochromatographic test format for O1 serotype Ogawa diagnosis and provide the need for better epidemic prevention and early response. DESIGN AND METHODS: The monoclonal antibodies were raised in conventional method and subsequently screened for a match pair. A variety of related and unrelated bacteria strains recruited were employed to test their sensitivity, specificity etc. by indirect ELISA. The human fecal samples were used to test the final lateral-flow device product to satisfy the measurement requirement. RESULTS: A new monoclonal antibody (McAb) pair, named IXiao₃G₆ and IXiao₁D₉, was generated, which is specifically against V. cholerae O1 serotype Ogawa. Additionally, we developed an immunochromatographic lateral flow device (LFD) using this McAb pair for the highly specific and rapid (5 min) detection of Ogawa. CONCLUSIONS: Our product has advantages of simplicity and precision, and can benefit the scene and elementary medical institutions.
Authors: Wilfredo R Matias; Fabrice E Julceus; Cademil Abelard; Leslie M Mayo-Smith; Molly F Franke; Jason B Harris; Louise C Ivers Journal: PLoS One Date: 2017-11-01 Impact factor: 3.240
Authors: Md Abu Sayeed; Kamrul Islam; Motaher Hossain; Noor Jahan Akter; Md Nur Alam; Nishat Sultana; Farhana Khanam; Meagan Kelly; Richelle C Charles; Pavol Kováč; Peng Xu; Jason R Andrews; Stephen B Calderwood; Jakia Amin; Edward T Ryan; Firdausi Qadri Journal: PLoS Negl Trop Dis Date: 2018-03-14