| Literature DB >> 24378219 |
Shekaraiah Devari1, Sundeep Jaglan2, Manjeet Kumar3, Ramesh Deshidi1, Santosh Guru4, Shashi Bhushan4, Manoj Kushwaha2, Ajai P Gupta2, Sumit G Gandhi4, Jai P Sharma5, Subhash C Taneja3, Ram A Vishwakarma3, Bhahwal Ali Shah6.
Abstract
Alternaria alternata, an endophytic fungus capable of producing capsaicin (1) was isolated from Capsicum annum. The endophyte was found to produce capsaicin upto three generations. Upscaling of the fermentation broth led to the isolation of one known and one compound characterized as 2,4-di-tert-butyl phenol (2) and alternariol-10-methyl ether (3) respectively. Compound 1 and 3 were identified and quantified using liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) system through multiple reaction monitoring (MRM). Furthermore, compound 3 displayed a range of cytotoxicity against a panel of human cancer cell lines and was found to induce apoptosis evidenced by Hoechst staining and loss of mitochondrial-membrane potential in HL-60 cells.Entities:
Keywords: (MRM)-LC–ESI–MS/MS; Alternaria alternata; Alternariol-10-methyl ether; Anti-cancer; Capsaicin
Mesh：
Substances：
Year: 2013 PMID： 24378219 DOI： 10.1016/j.phytochem.2013.12.001
Source DB: PubMed Journal: Phytochemistry ISSN： 0031-9422 Impact factor: 4.072